In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi
Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and inc...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:37578694187f4b9998d4ea5af22036af 2024-09-09T19:28:38+00:00 In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi Fátima Regina Vilani-Moreno Érika Mozer Ana Márcia Guedes de Sene Margarete de Oliveira Ferasçoli Tânia Cristina Pereira Márcia Garcia Miras Gláucia Heloísa de Paula Souza Andréa de Faria Fernandes Belone 2007-04-01T00:00:00Z https://doi.org/10.1590/S0036-46652007000200006 https://doaj.org/article/37578694187f4b9998d4ea5af22036af EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000200006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000200006 0036-4665 1678-9946 https://doaj.org/article/37578694187f4b9998d4ea5af22036af Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 2, Pp 97-101 (2007) Complement system Lacazia loboi Jorge Lobo's disease Immunofluorescence Skin lesions Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2007 ftdoajarticles https://doi.org/10.1590/S0036-46652007000200006 2024-08-05T17:49:30Z Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi. Considerando que não existe nenhum estudo avaliando a participação do sistema complemento (SC) na doença de Jorge Lobo e sua atividade sobre o fungo Lacazia loboi, realizamos o presente trabalho. Os fungos foram obtidos dos coxins plantares de camundongos BALB/c com índice de viabilidade de 48% e, em seguida, foram incubados com pool de soro inativado de pacientes ou com solução salina estéril (SSE) por 30 min, a 37 ºC. Os tubos foram incubados, por 2 h, com pool de soro AB+ sem inativar, inativado, diluído em EGTA-MgCl2 e EDTA. A viabilidade do L. loboi foi avaliada e a suspensão fúngica foi citocentrifugada. As lâminas foram submetidas à técnica de imunofluorescência empregando o anticorpo anti-C3 humano. Os resultados revelaram que 98% dos fungos ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 49 2 97 101 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Complement system Lacazia loboi Jorge Lobo's disease Immunofluorescence Skin lesions Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Complement system Lacazia loboi Jorge Lobo's disease Immunofluorescence Skin lesions Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Fátima Regina Vilani-Moreno Érika Mozer Ana Márcia Guedes de Sene Margarete de Oliveira Ferasçoli Tânia Cristina Pereira Márcia Garcia Miras Gláucia Heloísa de Paula Souza Andréa de Faria Fernandes Belone In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
topic_facet |
Complement system Lacazia loboi Jorge Lobo's disease Immunofluorescence Skin lesions Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Since there are no studies evaluating the participation of the complement system (CS) in Jorge Lobo's disease and its activity on the fungus Lacazia loboi, we carried out the present investigation. Fungal cells with a viability index of 48% were obtained from the footpads of BALB/c mice and incubated with a pool of inactivated serum from patients with the mycosis or with sterile saline for 30 min at 37 ºC. Next, the tubes were incubated for 2 h with a pool of noninactivated AB+ serum, inactivated serum, serum diluted in EGTA-MgCl2, and serum diluted in EDTA. The viability of L. loboi was evaluated and the fungal suspension was cytocentrifuged. The slides were submitted to immunofluorescence staining using human anti-C3 antibody. The results revealed that 98% of the fungi activated the CS by the alternative pathway and no significant difference in L. loboi viability was observed after CS activation. In parallel, frozen histological sections from 11 patients were analyzed regarding the presence of C3 and IgG by immunofluorescence staining. C3 and IgG deposits were observed in the fungal wall of 100% and 91% of the lesions evaluated, respectively. The results suggest that the CS and immunoglobulins may contribute to the defense mechanisms of the host against L. loboi. Considerando que não existe nenhum estudo avaliando a participação do sistema complemento (SC) na doença de Jorge Lobo e sua atividade sobre o fungo Lacazia loboi, realizamos o presente trabalho. Os fungos foram obtidos dos coxins plantares de camundongos BALB/c com índice de viabilidade de 48% e, em seguida, foram incubados com pool de soro inativado de pacientes ou com solução salina estéril (SSE) por 30 min, a 37 ºC. Os tubos foram incubados, por 2 h, com pool de soro AB+ sem inativar, inativado, diluído em EGTA-MgCl2 e EDTA. A viabilidade do L. loboi foi avaliada e a suspensão fúngica foi citocentrifugada. As lâminas foram submetidas à técnica de imunofluorescência empregando o anticorpo anti-C3 humano. Os resultados revelaram que 98% dos fungos ... |
format |
Article in Journal/Newspaper |
author |
Fátima Regina Vilani-Moreno Érika Mozer Ana Márcia Guedes de Sene Margarete de Oliveira Ferasçoli Tânia Cristina Pereira Márcia Garcia Miras Gláucia Heloísa de Paula Souza Andréa de Faria Fernandes Belone |
author_facet |
Fátima Regina Vilani-Moreno Érika Mozer Ana Márcia Guedes de Sene Margarete de Oliveira Ferasçoli Tânia Cristina Pereira Márcia Garcia Miras Gláucia Heloísa de Paula Souza Andréa de Faria Fernandes Belone |
author_sort |
Fátima Regina Vilani-Moreno |
title |
In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_short |
In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_full |
In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_fullStr |
In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_full_unstemmed |
In vitro and in situ activation of the complement system by the fungus Lacazia loboi Ativação in vitro e in situ do sistema complemento pelo fungo Lacazia loboi |
title_sort |
in vitro and in situ activation of the complement system by the fungus lacazia loboi ativação in vitro e in situ do sistema complemento pelo fungo lacazia loboi |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2007 |
url |
https://doi.org/10.1590/S0036-46652007000200006 https://doaj.org/article/37578694187f4b9998d4ea5af22036af |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 49, Iss 2, Pp 97-101 (2007) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652007000200006 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652007000200006 0036-4665 1678-9946 https://doaj.org/article/37578694187f4b9998d4ea5af22036af |
op_doi |
https://doi.org/10.1590/S0036-46652007000200006 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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49 |
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