Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice
Abstract Background In addition to pharmacovigilance and pharmaco-economic concerns, resistance to anti-malarial medicines has been documented in all classes of anti-malarials and this is further worsened by resistance to common insecticides by malaria vector, which is a major threat to malaria cont...
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ftdoajarticles:oai:doaj.org/article:37386cd07ede40c8b86af3d9ac3632e5 2023-05-15T15:11:53+02:00 Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice Eshetie Melese Birru Mestayet Geta Abyot Endale Gurmu 2017-05-01T00:00:00Z https://doi.org/10.1186/s12936-017-1853-5 https://doaj.org/article/37386cd07ede40c8b86af3d9ac3632e5 EN eng BMC http://link.springer.com/article/10.1186/s12936-017-1853-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1853-5 1475-2875 https://doaj.org/article/37386cd07ede40c8b86af3d9ac3632e5 Malaria Journal, Vol 16, Iss 1, Pp 1-7 (2017) Malaria Herbal medicine Indigofera spicata Plasmodium berghei In vivo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2017 ftdoajarticles https://doi.org/10.1186/s12936-017-1853-5 2022-12-31T02:05:30Z Abstract Background In addition to pharmacovigilance and pharmaco-economic concerns, resistance to anti-malarial medicines has been documented in all classes of anti-malarials and this is further worsened by resistance to common insecticides by malaria vector, which is a major threat to malaria control. As a means of facing the challenges of searching for new anti-malarial agents, the current study focused on evaluation of anti-malarial activity of root extract of Indigofera spicata. Methods Chloroquine-sensitive rodent malaria parasite, Plasmodium berghei (ANKA strain) was used to infect the Swiss Albino mice in 4-day suppressive and curative models. The crude hydromethanolic root extract of I. spicata at 200, 400 and 600 mg/kg doses was administered to a group of five mice. Important parameters, such as level of parasitaemia, packed cell volume (PCV), survival time, and body weight were determined and the significance of the differences between mean values of the five groups was analysed by one-way ANOVA followed by post hoc Tukey’s Multiple Comparison test. Results In both the suppressive and curative models, 400 and 600 mg/kg doses of the extract suppressed the level of parasitaemia significantly (p < 0.001) compared to the vehicle-treated groups, 34.93 and 53.42%, respectively. However, only the mice which were treated with the 600 mg/kg dose of the extract had significant difference in their mean survival time. In other parameters, namely PCV and mean body weight, there was no statistically significant difference between the extract-treated groups when compared to the negative control. Conclusions This study revealed that the root extract of I. spicata possesses anti-malarial activity and necessitates further scientific validation. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 16 1 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Herbal medicine Indigofera spicata Plasmodium berghei In vivo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Herbal medicine Indigofera spicata Plasmodium berghei In vivo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Eshetie Melese Birru Mestayet Geta Abyot Endale Gurmu Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice |
topic_facet |
Malaria Herbal medicine Indigofera spicata Plasmodium berghei In vivo Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In addition to pharmacovigilance and pharmaco-economic concerns, resistance to anti-malarial medicines has been documented in all classes of anti-malarials and this is further worsened by resistance to common insecticides by malaria vector, which is a major threat to malaria control. As a means of facing the challenges of searching for new anti-malarial agents, the current study focused on evaluation of anti-malarial activity of root extract of Indigofera spicata. Methods Chloroquine-sensitive rodent malaria parasite, Plasmodium berghei (ANKA strain) was used to infect the Swiss Albino mice in 4-day suppressive and curative models. The crude hydromethanolic root extract of I. spicata at 200, 400 and 600 mg/kg doses was administered to a group of five mice. Important parameters, such as level of parasitaemia, packed cell volume (PCV), survival time, and body weight were determined and the significance of the differences between mean values of the five groups was analysed by one-way ANOVA followed by post hoc Tukey’s Multiple Comparison test. Results In both the suppressive and curative models, 400 and 600 mg/kg doses of the extract suppressed the level of parasitaemia significantly (p < 0.001) compared to the vehicle-treated groups, 34.93 and 53.42%, respectively. However, only the mice which were treated with the 600 mg/kg dose of the extract had significant difference in their mean survival time. In other parameters, namely PCV and mean body weight, there was no statistically significant difference between the extract-treated groups when compared to the negative control. Conclusions This study revealed that the root extract of I. spicata possesses anti-malarial activity and necessitates further scientific validation. |
format |
Article in Journal/Newspaper |
author |
Eshetie Melese Birru Mestayet Geta Abyot Endale Gurmu |
author_facet |
Eshetie Melese Birru Mestayet Geta Abyot Endale Gurmu |
author_sort |
Eshetie Melese Birru |
title |
Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice |
title_short |
Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice |
title_full |
Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice |
title_fullStr |
Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice |
title_full_unstemmed |
Antiplasmodial activity of Indigofera spicata root extract against Plasmodium berghei infection in mice |
title_sort |
antiplasmodial activity of indigofera spicata root extract against plasmodium berghei infection in mice |
publisher |
BMC |
publishDate |
2017 |
url |
https://doi.org/10.1186/s12936-017-1853-5 https://doaj.org/article/37386cd07ede40c8b86af3d9ac3632e5 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 16, Iss 1, Pp 1-7 (2017) |
op_relation |
http://link.springer.com/article/10.1186/s12936-017-1853-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-017-1853-5 1475-2875 https://doaj.org/article/37386cd07ede40c8b86af3d9ac3632e5 |
op_doi |
https://doi.org/10.1186/s12936-017-1853-5 |
container_title |
Malaria Journal |
container_volume |
16 |
container_issue |
1 |
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1766342674239979520 |