Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP
Considering the importance of merozoite surface proteins (MSPs) as vaccine candidates, this study was conducted to investigate the polymorphism and genetic diversity of Plasmodium vivax merozoite surface protein 3-alpha (PvMSP-3α) in Thailand. To analyze genetic diversity, 118 blood samples containi...
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ftdoajarticles:oai:doaj.org/article:37304eac88fc406aa62b230cb1bf71ce 2023-09-05T13:17:45+02:00 Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP Kanyanan Kritsiriwuthinan Warunee Ngrenngarmlert Rapatbhorn Patrapuvich Supaksajee Phuagthong Kantima Choosang 2023-01-01T00:00:00Z https://doi.org/10.1155/2023/8855171 https://doaj.org/article/37304eac88fc406aa62b230cb1bf71ce EN eng Hindawi Limited http://dx.doi.org/10.1155/2023/8855171 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/8855171 https://doaj.org/article/37304eac88fc406aa62b230cb1bf71ce Journal of Tropical Medicine, Vol 2023 (2023) Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.1155/2023/8855171 2023-08-20T00:33:55Z Considering the importance of merozoite surface proteins (MSPs) as vaccine candidates, this study was conducted to investigate the polymorphism and genetic diversity of Plasmodium vivax merozoite surface protein 3-alpha (PvMSP-3α) in Thailand. To analyze genetic diversity, 118 blood samples containing P. vivax were collected from four malaria-endemic areas in western and southern Thailand. The DNA was extracted and amplified for the PvMSP-3α gene using nested PCR. The PCR products were genotyped by PCR-RFLP with Hha I and Alu I restriction enzymes. The combination patterns of Hha I and Alu I RFLP were used to identify allelic variants. Genetic evaluation and phylogenic analysis were performed on 13 sequences, including 10 sequences from our study and 3 sequences from GenBank. The results revealed three major types of PvMSP-3α, 91.5% allelic type A (∼1.8 kb), 5.1% allelic type B (∼1.5 kb), and 3.4% allelic type C (∼1.2 kb), were detected based on PCR product size with different frequencies. Among all PvMSP-3α, 19 allelic subtypes with Hha I RFLP patterns were distinguished and 6 allelic subtypes with Alu I RFLP patterns were identified. Of these samples, 73 (61%) and 42 (35.6%) samples were defined as monoallelic subtype infection by Hha I and Alu I PCR-RFLP, respectively, whereas 77 (65.3%) samples were determined to be mixed-allelic subtype infection by the combination patterns of Hha I and Alu I RFLP. These results strongly indicate that PvMSP-3α gene is highly polymorphic, particularly in blood samples collected from the Thai-Myanmar border area (the western part of Thailand). The combination patterns of Hha I and Alu I RFLP of the PvMSP-3α gene could be considered for use as molecular epidemiologic markers for genotyping P. vivax isolates in Thailand. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Tropical Medicine 2023 1 8 |
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Arctic medicine. Tropical medicine RC955-962 |
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Arctic medicine. Tropical medicine RC955-962 Kanyanan Kritsiriwuthinan Warunee Ngrenngarmlert Rapatbhorn Patrapuvich Supaksajee Phuagthong Kantima Choosang Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 |
description |
Considering the importance of merozoite surface proteins (MSPs) as vaccine candidates, this study was conducted to investigate the polymorphism and genetic diversity of Plasmodium vivax merozoite surface protein 3-alpha (PvMSP-3α) in Thailand. To analyze genetic diversity, 118 blood samples containing P. vivax were collected from four malaria-endemic areas in western and southern Thailand. The DNA was extracted and amplified for the PvMSP-3α gene using nested PCR. The PCR products were genotyped by PCR-RFLP with Hha I and Alu I restriction enzymes. The combination patterns of Hha I and Alu I RFLP were used to identify allelic variants. Genetic evaluation and phylogenic analysis were performed on 13 sequences, including 10 sequences from our study and 3 sequences from GenBank. The results revealed three major types of PvMSP-3α, 91.5% allelic type A (∼1.8 kb), 5.1% allelic type B (∼1.5 kb), and 3.4% allelic type C (∼1.2 kb), were detected based on PCR product size with different frequencies. Among all PvMSP-3α, 19 allelic subtypes with Hha I RFLP patterns were distinguished and 6 allelic subtypes with Alu I RFLP patterns were identified. Of these samples, 73 (61%) and 42 (35.6%) samples were defined as monoallelic subtype infection by Hha I and Alu I PCR-RFLP, respectively, whereas 77 (65.3%) samples were determined to be mixed-allelic subtype infection by the combination patterns of Hha I and Alu I RFLP. These results strongly indicate that PvMSP-3α gene is highly polymorphic, particularly in blood samples collected from the Thai-Myanmar border area (the western part of Thailand). The combination patterns of Hha I and Alu I RFLP of the PvMSP-3α gene could be considered for use as molecular epidemiologic markers for genotyping P. vivax isolates in Thailand. |
format |
Article in Journal/Newspaper |
author |
Kanyanan Kritsiriwuthinan Warunee Ngrenngarmlert Rapatbhorn Patrapuvich Supaksajee Phuagthong Kantima Choosang |
author_facet |
Kanyanan Kritsiriwuthinan Warunee Ngrenngarmlert Rapatbhorn Patrapuvich Supaksajee Phuagthong Kantima Choosang |
author_sort |
Kanyanan Kritsiriwuthinan |
title |
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP |
title_short |
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP |
title_full |
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP |
title_fullStr |
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP |
title_full_unstemmed |
Distinct Allelic Diversity of Plasmodium vivax Merozoite Surface Protein 3-Alpha (PvMSP-3α) Gene in Thailand Using PCR-RFLP |
title_sort |
distinct allelic diversity of plasmodium vivax merozoite surface protein 3-alpha (pvmsp-3α) gene in thailand using pcr-rflp |
publisher |
Hindawi Limited |
publishDate |
2023 |
url |
https://doi.org/10.1155/2023/8855171 https://doaj.org/article/37304eac88fc406aa62b230cb1bf71ce |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Tropical Medicine, Vol 2023 (2023) |
op_relation |
http://dx.doi.org/10.1155/2023/8855171 https://doaj.org/toc/1687-9694 1687-9694 doi:10.1155/2023/8855171 https://doaj.org/article/37304eac88fc406aa62b230cb1bf71ce |
op_doi |
https://doi.org/10.1155/2023/8855171 |
container_title |
Journal of Tropical Medicine |
container_volume |
2023 |
container_start_page |
1 |
op_container_end_page |
8 |
_version_ |
1776198806175481856 |