IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection.
Th9 cells are a subset of CD4+ T cells that express the protoypical cytokine, IL-9. Th9 cells are known to effect protective immunity in animal models of intestinal helminth infections. However, the role of Th9 cells in human intestinal helminth infections has never been examined.To examine the role...
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ftdoajarticles:oai:doaj.org/article:372cc18963744cc68aee9a3d1690b3f1 2023-05-15T15:12:03+02:00 IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection. Rajamanickam Anuradha Saravanan Munisankar Yukthi Bhootra Jeeva Jagannathan Chandrakumar Dolla Paul Kumaran Thomas B Nutman Subash Babu 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004317 https://doaj.org/article/372cc18963744cc68aee9a3d1690b3f1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4701466?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004317 https://doaj.org/article/372cc18963744cc68aee9a3d1690b3f1 PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004317 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004317 2022-12-31T03:54:39Z Th9 cells are a subset of CD4+ T cells that express the protoypical cytokine, IL-9. Th9 cells are known to effect protective immunity in animal models of intestinal helminth infections. However, the role of Th9 cells in human intestinal helminth infections has never been examined.To examine the role of Th9 cells in Strongyloidis stercoralis (Ss), a common intestinal helminth infection, we compared the frequency of Th9 expressing IL-9 either singly (mono-functional) or co-expressing IL-4 or IL-10 (dual-functional) in Ss-infected individuals (INF) to frequencies in uninfected (UN) individuals.INF individuals exhibited a significant increase in the spontaneously expressed and/or antigen specific frequencies of both mono- and dual-functional Th9 cells as well as Th2 cells expressing IL-9 compared to UN. The differences in Th9 induction between INF and UN individuals was predominantly antigen-specific as the differences were no longer seen following control antigen or mitogen stimulation. In addition, the increased frequency of Th9 cells in response to parasite antigens was dependent on IL-10 and TGFx since neutralization of either of these cytokines resulted in diminished Th9 frequencies. Finally, following successful treatment of Ss infection, the frequencies of antigen-specific Th9 cells diminished in INF individuals, suggesting a role for the Th9 response in active Ss infection. Moreover, IL-9 levels in whole blood culture supernatants following Ss antigen stimulation were higher in INF compared to UN individuals.Thus, Ss infection is characterized by an IL-10- and TGFβ dependent expansion of Th9 cells, an expansion found to reversible by anti-helmintic treatment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 1 e0004317 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rajamanickam Anuradha Saravanan Munisankar Yukthi Bhootra Jeeva Jagannathan Chandrakumar Dolla Paul Kumaran Thomas B Nutman Subash Babu IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Th9 cells are a subset of CD4+ T cells that express the protoypical cytokine, IL-9. Th9 cells are known to effect protective immunity in animal models of intestinal helminth infections. However, the role of Th9 cells in human intestinal helminth infections has never been examined.To examine the role of Th9 cells in Strongyloidis stercoralis (Ss), a common intestinal helminth infection, we compared the frequency of Th9 expressing IL-9 either singly (mono-functional) or co-expressing IL-4 or IL-10 (dual-functional) in Ss-infected individuals (INF) to frequencies in uninfected (UN) individuals.INF individuals exhibited a significant increase in the spontaneously expressed and/or antigen specific frequencies of both mono- and dual-functional Th9 cells as well as Th2 cells expressing IL-9 compared to UN. The differences in Th9 induction between INF and UN individuals was predominantly antigen-specific as the differences were no longer seen following control antigen or mitogen stimulation. In addition, the increased frequency of Th9 cells in response to parasite antigens was dependent on IL-10 and TGFx since neutralization of either of these cytokines resulted in diminished Th9 frequencies. Finally, following successful treatment of Ss infection, the frequencies of antigen-specific Th9 cells diminished in INF individuals, suggesting a role for the Th9 response in active Ss infection. Moreover, IL-9 levels in whole blood culture supernatants following Ss antigen stimulation were higher in INF compared to UN individuals.Thus, Ss infection is characterized by an IL-10- and TGFβ dependent expansion of Th9 cells, an expansion found to reversible by anti-helmintic treatment. |
format |
Article in Journal/Newspaper |
author |
Rajamanickam Anuradha Saravanan Munisankar Yukthi Bhootra Jeeva Jagannathan Chandrakumar Dolla Paul Kumaran Thomas B Nutman Subash Babu |
author_facet |
Rajamanickam Anuradha Saravanan Munisankar Yukthi Bhootra Jeeva Jagannathan Chandrakumar Dolla Paul Kumaran Thomas B Nutman Subash Babu |
author_sort |
Rajamanickam Anuradha |
title |
IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection. |
title_short |
IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection. |
title_full |
IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection. |
title_fullStr |
IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection. |
title_full_unstemmed |
IL-10- and TGFβ-mediated Th9 Responses in a Human Helminth Infection. |
title_sort |
il-10- and tgfβ-mediated th9 responses in a human helminth infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004317 https://doaj.org/article/372cc18963744cc68aee9a3d1690b3f1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004317 (2016) |
op_relation |
http://europepmc.org/articles/PMC4701466?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004317 https://doaj.org/article/372cc18963744cc68aee9a3d1690b3f1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004317 |
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PLOS Neglected Tropical Diseases |
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10 |
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1 |
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e0004317 |
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1766342797834584064 |