Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species
Species identification of unknown biological samples is of fundamental importance for forensic applications, especially in crime detection, poaching, and illegal trade of endangered animals as well as meat fraud. In this study, a novel panel was developed to simultaneously identify 10 different anim...
Published in: | Frontiers in Genetics |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fgene.2020.01005 https://doaj.org/article/36f8ad9c37c54a5f839fa1e078985a49 |
id |
ftdoajarticles:oai:doaj.org/article:36f8ad9c37c54a5f839fa1e078985a49 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:36f8ad9c37c54a5f839fa1e078985a49 2023-05-15T15:50:31+02:00 Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species Wei Cui Xiaoye Jin Yuxin Guo Chong Chen Wenqing Zhang Yijie Wang Jiangwei Lan Bofeng Zhu 2020-09-01T00:00:00Z https://doi.org/10.3389/fgene.2020.01005 https://doaj.org/article/36f8ad9c37c54a5f839fa1e078985a49 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fgene.2020.01005/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2020.01005 https://doaj.org/article/36f8ad9c37c54a5f839fa1e078985a49 Frontiers in Genetics, Vol 11 (2020) species identification meat fraud developmental validation forensic science short tandem repeat Genetics QH426-470 article 2020 ftdoajarticles https://doi.org/10.3389/fgene.2020.01005 2022-12-31T14:02:11Z Species identification of unknown biological samples is of fundamental importance for forensic applications, especially in crime detection, poaching, and illegal trade of endangered animals as well as meat fraud. In this study, a novel panel was developed to simultaneously identify 10 different animal species (Gallus domesticus, Anas platyrhynchos domesticus, Ovis aries, Sus scrofa domesticus, Bos taurus, Equus caballus, Columba livia domestica, Rattus norvegicus, Mus musculus, and Canis lupus familiaris) and human beings by amplifying 22 short tandem repeat (STR) loci in a multiplex PCR using a set of five fluorescently labeled dyes. This novel 22-STR panel was validated by optimization of PCR conditions as well as species specificity, sensitivity, reproducibility, precision, DNA mixture, and tissue/organ consistency. The results of developmental validation showed that the 22-STR loci achieved high species specificity among 10 animal species and human beings, and the sensitivity of this panel was 0.09 ng. This 22-STR panel identified different meats in mixed samples, and the minimum detected mixture ratio in the current test was 10% (0.1 ng/1 ng). This sensitive, accurate, and specific 22-STR panel can be used for forensic species identification and the detection of meat fraud and adulteration. Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Frontiers in Genetics 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
species identification meat fraud developmental validation forensic science short tandem repeat Genetics QH426-470 |
spellingShingle |
species identification meat fraud developmental validation forensic science short tandem repeat Genetics QH426-470 Wei Cui Xiaoye Jin Yuxin Guo Chong Chen Wenqing Zhang Yijie Wang Jiangwei Lan Bofeng Zhu Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species |
topic_facet |
species identification meat fraud developmental validation forensic science short tandem repeat Genetics QH426-470 |
description |
Species identification of unknown biological samples is of fundamental importance for forensic applications, especially in crime detection, poaching, and illegal trade of endangered animals as well as meat fraud. In this study, a novel panel was developed to simultaneously identify 10 different animal species (Gallus domesticus, Anas platyrhynchos domesticus, Ovis aries, Sus scrofa domesticus, Bos taurus, Equus caballus, Columba livia domestica, Rattus norvegicus, Mus musculus, and Canis lupus familiaris) and human beings by amplifying 22 short tandem repeat (STR) loci in a multiplex PCR using a set of five fluorescently labeled dyes. This novel 22-STR panel was validated by optimization of PCR conditions as well as species specificity, sensitivity, reproducibility, precision, DNA mixture, and tissue/organ consistency. The results of developmental validation showed that the 22-STR loci achieved high species specificity among 10 animal species and human beings, and the sensitivity of this panel was 0.09 ng. This 22-STR panel identified different meats in mixed samples, and the minimum detected mixture ratio in the current test was 10% (0.1 ng/1 ng). This sensitive, accurate, and specific 22-STR panel can be used for forensic species identification and the detection of meat fraud and adulteration. |
format |
Article in Journal/Newspaper |
author |
Wei Cui Xiaoye Jin Yuxin Guo Chong Chen Wenqing Zhang Yijie Wang Jiangwei Lan Bofeng Zhu |
author_facet |
Wei Cui Xiaoye Jin Yuxin Guo Chong Chen Wenqing Zhang Yijie Wang Jiangwei Lan Bofeng Zhu |
author_sort |
Wei Cui |
title |
Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species |
title_short |
Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species |
title_full |
Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species |
title_fullStr |
Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species |
title_full_unstemmed |
Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species |
title_sort |
development and validation of a novel five-dye short tandem repeat panel for forensic identification of 11 species |
publisher |
Frontiers Media S.A. |
publishDate |
2020 |
url |
https://doi.org/10.3389/fgene.2020.01005 https://doaj.org/article/36f8ad9c37c54a5f839fa1e078985a49 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Frontiers in Genetics, Vol 11 (2020) |
op_relation |
https://www.frontiersin.org/article/10.3389/fgene.2020.01005/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2020.01005 https://doaj.org/article/36f8ad9c37c54a5f839fa1e078985a49 |
op_doi |
https://doi.org/10.3389/fgene.2020.01005 |
container_title |
Frontiers in Genetics |
container_volume |
11 |
_version_ |
1766385482518757376 |