Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures
The percentage of cells dividing in a specific tissue of individual larvae can be estimated by analyzing DNA per cell by flow cytometry. An experimental test was carried out with cod (Gadus morhua) larvae, with brain as the target tissue, to validate this technique as an appropriate growth index for...
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Consejo Superior de Investigaciones Científicas
2007
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ftdoajarticles:oai:doaj.org/article:36d998cbf0ec4d93a46fbb0a86c55284 2023-05-15T16:19:16+02:00 Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures Rafael González-Quirós Iyziar Munuera Arild Folkvord 2007-09-01T00:00:00Z https://doi.org/10.3989/scimar.2007.71n3485 https://doaj.org/article/36d998cbf0ec4d93a46fbb0a86c55284 EN eng Consejo Superior de Investigaciones Científicas http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/53 https://doaj.org/toc/0214-8358 https://doaj.org/toc/1886-8134 0214-8358 1886-8134 doi:10.3989/scimar.2007.71n3485 https://doaj.org/article/36d998cbf0ec4d93a46fbb0a86c55284 Scientia Marina, Vol 71, Iss 3, Pp 485-497 (2007) brain cell cycle cod larvae flow cytometry food growth index temperature Aquaculture. Fisheries. Angling SH1-691 article 2007 ftdoajarticles https://doi.org/10.3989/scimar.2007.71n3485 2022-12-31T07:02:59Z The percentage of cells dividing in a specific tissue of individual larvae can be estimated by analyzing DNA per cell by flow cytometry. An experimental test was carried out with cod (Gadus morhua) larvae, with brain as the target tissue, to validate this technique as an appropriate growth index for larval fish. Standard length (SL), myotome height, and %S-phase (% of cells in the S-phase of the cell-division cycle) variability were analyzed, with temperature (6 and 10°C), food level (high- and no-food) and larval developmental stage (first feeding, pre-metamorphosis and post-metamorphosis) as independent factors. Cod larvae grew faster (in SL) and presented a higher %S-phase under high-food conditions. Larval SL increased with temperature in rearing and experimental tanks. However, there was a significant interaction between temperature and food in the %S-phase. There were no significant differences in the %S-phase between 6 and 10°C at high-food levels. We suggest that this result is a consequence of temperature-dependency of the duration of the cell cycle. In the absence of food, larvae at 10ºC had a lower %S-phase than larvae at 6°C, which may be related to increased metabolic costs with increasing temperature. Considering the effect of temperature, the mean % S-phase explained 74% of the variability in the estimated standard growth rate. Article in Journal/Newspaper Gadus morhua Directory of Open Access Journals: DOAJ Articles Scientia Marina 71 3 485 497 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
brain cell cycle cod larvae flow cytometry food growth index temperature Aquaculture. Fisheries. Angling SH1-691 |
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brain cell cycle cod larvae flow cytometry food growth index temperature Aquaculture. Fisheries. Angling SH1-691 Rafael González-Quirós Iyziar Munuera Arild Folkvord Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
topic_facet |
brain cell cycle cod larvae flow cytometry food growth index temperature Aquaculture. Fisheries. Angling SH1-691 |
description |
The percentage of cells dividing in a specific tissue of individual larvae can be estimated by analyzing DNA per cell by flow cytometry. An experimental test was carried out with cod (Gadus morhua) larvae, with brain as the target tissue, to validate this technique as an appropriate growth index for larval fish. Standard length (SL), myotome height, and %S-phase (% of cells in the S-phase of the cell-division cycle) variability were analyzed, with temperature (6 and 10°C), food level (high- and no-food) and larval developmental stage (first feeding, pre-metamorphosis and post-metamorphosis) as independent factors. Cod larvae grew faster (in SL) and presented a higher %S-phase under high-food conditions. Larval SL increased with temperature in rearing and experimental tanks. However, there was a significant interaction between temperature and food in the %S-phase. There were no significant differences in the %S-phase between 6 and 10°C at high-food levels. We suggest that this result is a consequence of temperature-dependency of the duration of the cell cycle. In the absence of food, larvae at 10ºC had a lower %S-phase than larvae at 6°C, which may be related to increased metabolic costs with increasing temperature. Considering the effect of temperature, the mean % S-phase explained 74% of the variability in the estimated standard growth rate. |
format |
Article in Journal/Newspaper |
author |
Rafael González-Quirós Iyziar Munuera Arild Folkvord |
author_facet |
Rafael González-Quirós Iyziar Munuera Arild Folkvord |
author_sort |
Rafael González-Quirós |
title |
Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_short |
Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_full |
Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_fullStr |
Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_full_unstemmed |
Cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
title_sort |
cell cycle analysis of brain cells as a growth index in larval cod at different feeding conditions and temperatures |
publisher |
Consejo Superior de Investigaciones Científicas |
publishDate |
2007 |
url |
https://doi.org/10.3989/scimar.2007.71n3485 https://doaj.org/article/36d998cbf0ec4d93a46fbb0a86c55284 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Scientia Marina, Vol 71, Iss 3, Pp 485-497 (2007) |
op_relation |
http://scientiamarina.revistas.csic.es/index.php/scientiamarina/article/view/53 https://doaj.org/toc/0214-8358 https://doaj.org/toc/1886-8134 0214-8358 1886-8134 doi:10.3989/scimar.2007.71n3485 https://doaj.org/article/36d998cbf0ec4d93a46fbb0a86c55284 |
op_doi |
https://doi.org/10.3989/scimar.2007.71n3485 |
container_title |
Scientia Marina |
container_volume |
71 |
container_issue |
3 |
container_start_page |
485 |
op_container_end_page |
497 |
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1766005637019336704 |