Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover
The common cuttlefish (Sepia officinalis), a dominant species in the north-east Atlantic ocean and Mediterranean Sea, is potentially subject to hypoxic conditions due to eutrophication of coastal waters and intensive aquaculture. Here we initiate studies on the biochemical response to an anticipated...
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ftdoajarticles:oai:doaj.org/article:36aa63fa96924acaae2cd406a3081995 2023-05-15T17:38:35+02:00 Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover Juan C. Capaz Louise Tunnah Tyson J. MacCormack Simon G. Lamarre Antonio V. Sykes William R. Driedzic 2017-05-01T00:00:00Z https://doi.org/10.3389/fphys.2017.00344 https://doaj.org/article/36aa63fa96924acaae2cd406a3081995 EN eng Frontiers Media S.A. https://www.frontiersin.org/article/10.3389/fphys.2017.00344/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2017.00344 https://doaj.org/article/36aa63fa96924acaae2cd406a3081995 Frontiers in Physiology, Vol 8 (2017) European cuttlefish Sepia officinalis HSP70 octopine polyubiquitinated protein ventilation frequency Physiology QP1-981 article 2017 ftdoajarticles https://doi.org/10.3389/fphys.2017.00344 2022-12-31T16:00:44Z The common cuttlefish (Sepia officinalis), a dominant species in the north-east Atlantic ocean and Mediterranean Sea, is potentially subject to hypoxic conditions due to eutrophication of coastal waters and intensive aquaculture. Here we initiate studies on the biochemical response to an anticipated level of hypoxia. Cuttlefish challenged for 1 h at an oxygen level of 50% dissolved oxygen saturation showed a decrease in oxygen consumption of 37% associated with an 85% increase in ventilation rate. Octopine levels were increased to a small but significant level in mantle, whereas there was no change in gill or heart. There were no changes in mantle free glucose or glycogen levels. Similarly, the hypoxic period did not result in changes in HSP70 or polyubiquinated protein levels in mantle, gill, or heart. As such, it appears that although there was a decrease in metabolic rate there was only a minor increase in anaerobic metabolism as evidenced by octopine accumulation and no biochemical changes that are hallmarks of alterations in protein trafficking. Experiments with isolated preparations of mantle, gill, and heart revealed that pharmacological inhibition of protein synthesis could decrease oxygen consumption by 32 to 42% or Na+/K+ ATPase activity by 24 to 54% dependent upon tissue type. We propose that the decrease in whole animal oxygen consumption was potentially the result of controlled decreases in the energy demanding processes of both protein synthesis and Na+/K+ ATPase activity. Article in Journal/Newspaper North East Atlantic Directory of Open Access Journals: DOAJ Articles Frontiers in Physiology 8 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
European cuttlefish Sepia officinalis HSP70 octopine polyubiquitinated protein ventilation frequency Physiology QP1-981 |
spellingShingle |
European cuttlefish Sepia officinalis HSP70 octopine polyubiquitinated protein ventilation frequency Physiology QP1-981 Juan C. Capaz Louise Tunnah Tyson J. MacCormack Simon G. Lamarre Antonio V. Sykes William R. Driedzic Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover |
topic_facet |
European cuttlefish Sepia officinalis HSP70 octopine polyubiquitinated protein ventilation frequency Physiology QP1-981 |
description |
The common cuttlefish (Sepia officinalis), a dominant species in the north-east Atlantic ocean and Mediterranean Sea, is potentially subject to hypoxic conditions due to eutrophication of coastal waters and intensive aquaculture. Here we initiate studies on the biochemical response to an anticipated level of hypoxia. Cuttlefish challenged for 1 h at an oxygen level of 50% dissolved oxygen saturation showed a decrease in oxygen consumption of 37% associated with an 85% increase in ventilation rate. Octopine levels were increased to a small but significant level in mantle, whereas there was no change in gill or heart. There were no changes in mantle free glucose or glycogen levels. Similarly, the hypoxic period did not result in changes in HSP70 or polyubiquinated protein levels in mantle, gill, or heart. As such, it appears that although there was a decrease in metabolic rate there was only a minor increase in anaerobic metabolism as evidenced by octopine accumulation and no biochemical changes that are hallmarks of alterations in protein trafficking. Experiments with isolated preparations of mantle, gill, and heart revealed that pharmacological inhibition of protein synthesis could decrease oxygen consumption by 32 to 42% or Na+/K+ ATPase activity by 24 to 54% dependent upon tissue type. We propose that the decrease in whole animal oxygen consumption was potentially the result of controlled decreases in the energy demanding processes of both protein synthesis and Na+/K+ ATPase activity. |
format |
Article in Journal/Newspaper |
author |
Juan C. Capaz Louise Tunnah Tyson J. MacCormack Simon G. Lamarre Antonio V. Sykes William R. Driedzic |
author_facet |
Juan C. Capaz Louise Tunnah Tyson J. MacCormack Simon G. Lamarre Antonio V. Sykes William R. Driedzic |
author_sort |
Juan C. Capaz |
title |
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover |
title_short |
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover |
title_full |
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover |
title_fullStr |
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover |
title_full_unstemmed |
Hypoxic Induced Decrease in Oxygen Consumption in Cuttlefish (Sepia officinalis) Is Associated with Minor Increases in Mantle Octopine but No Changes in Markers of Protein Turnover |
title_sort |
hypoxic induced decrease in oxygen consumption in cuttlefish (sepia officinalis) is associated with minor increases in mantle octopine but no changes in markers of protein turnover |
publisher |
Frontiers Media S.A. |
publishDate |
2017 |
url |
https://doi.org/10.3389/fphys.2017.00344 https://doaj.org/article/36aa63fa96924acaae2cd406a3081995 |
genre |
North East Atlantic |
genre_facet |
North East Atlantic |
op_source |
Frontiers in Physiology, Vol 8 (2017) |
op_relation |
https://www.frontiersin.org/article/10.3389/fphys.2017.00344/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2017.00344 https://doaj.org/article/36aa63fa96924acaae2cd406a3081995 |
op_doi |
https://doi.org/10.3389/fphys.2017.00344 |
container_title |
Frontiers in Physiology |
container_volume |
8 |
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1766139076243619840 |