Protective immunity by an engineered DNA vaccine for Mayaro virus.
Mayaro virus (MAYV) of the genus alphavirus is a mosquito-transmitted emerging infectious disease that causes an acute febrile illness, rash, headaches, and nausea that may turn into incapacitating, persistent arthralgias in some victims. Since its discovery in Trinidad in 1954, cases of MAYV infect...
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ftdoajarticles:oai:doaj.org/article:365111ea733342ab82ae66dc7897d8a4 2023-05-15T15:07:05+02:00 Protective immunity by an engineered DNA vaccine for Mayaro virus. Hyeree Choi Sagar B Kudchodkar Emma L Reuschel Kanika Asija Piyush Borole Michelle Ho Krzysztof Wojtak Charles Reed Stephanie Ramos Nathen E Bopp Patricia V Aguilar Scott C Weaver J Joseph Kim Laurent Humeau Pablo Tebas David B Weiner Kar Muthumani 2019-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007042 https://doaj.org/article/365111ea733342ab82ae66dc7897d8a4 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007042 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007042 https://doaj.org/article/365111ea733342ab82ae66dc7897d8a4 PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007042 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007042 2023-01-15T01:29:52Z Mayaro virus (MAYV) of the genus alphavirus is a mosquito-transmitted emerging infectious disease that causes an acute febrile illness, rash, headaches, and nausea that may turn into incapacitating, persistent arthralgias in some victims. Since its discovery in Trinidad in 1954, cases of MAYV infection have largely been confined there and to the northern countries of South America, but recently, MAYV cases have been reported in some island nations in the Caribbean Sea. Accompanying these reports is evidence that new vectors, including Aedes spp. mosquitos, recently implicated in the global spread of Zika and chikungunya viruses, are competent for MAYV transmission, which, if true, could facilitate the spread of MAYV beyond its current range. Despite its status as an emerging virus, there are no licensed vaccines to prevent MAYV infection nor therapeutics to treat it. Here, we describe the development and testing of a novel DNA vaccine, scMAYV-E, that encodes a synthetically-designed consensus MAYV envelope sequence. In vivo electroporation-enhanced immunization of mice with this vaccine induced potent humoral responses including neutralizing antibodies as well as robust T-cell responses to multiple epitopes in the MAYV envelope. Importantly, these scMAYV-E-induced immune responses protected susceptible mice from morbidity and mortality following a MAYV challenge. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Trinidad ENVELOPE(-60.734,-60.734,-63.816,-63.816) PLOS Neglected Tropical Diseases 13 2 e0007042 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Hyeree Choi Sagar B Kudchodkar Emma L Reuschel Kanika Asija Piyush Borole Michelle Ho Krzysztof Wojtak Charles Reed Stephanie Ramos Nathen E Bopp Patricia V Aguilar Scott C Weaver J Joseph Kim Laurent Humeau Pablo Tebas David B Weiner Kar Muthumani Protective immunity by an engineered DNA vaccine for Mayaro virus. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mayaro virus (MAYV) of the genus alphavirus is a mosquito-transmitted emerging infectious disease that causes an acute febrile illness, rash, headaches, and nausea that may turn into incapacitating, persistent arthralgias in some victims. Since its discovery in Trinidad in 1954, cases of MAYV infection have largely been confined there and to the northern countries of South America, but recently, MAYV cases have been reported in some island nations in the Caribbean Sea. Accompanying these reports is evidence that new vectors, including Aedes spp. mosquitos, recently implicated in the global spread of Zika and chikungunya viruses, are competent for MAYV transmission, which, if true, could facilitate the spread of MAYV beyond its current range. Despite its status as an emerging virus, there are no licensed vaccines to prevent MAYV infection nor therapeutics to treat it. Here, we describe the development and testing of a novel DNA vaccine, scMAYV-E, that encodes a synthetically-designed consensus MAYV envelope sequence. In vivo electroporation-enhanced immunization of mice with this vaccine induced potent humoral responses including neutralizing antibodies as well as robust T-cell responses to multiple epitopes in the MAYV envelope. Importantly, these scMAYV-E-induced immune responses protected susceptible mice from morbidity and mortality following a MAYV challenge. |
format |
Article in Journal/Newspaper |
author |
Hyeree Choi Sagar B Kudchodkar Emma L Reuschel Kanika Asija Piyush Borole Michelle Ho Krzysztof Wojtak Charles Reed Stephanie Ramos Nathen E Bopp Patricia V Aguilar Scott C Weaver J Joseph Kim Laurent Humeau Pablo Tebas David B Weiner Kar Muthumani |
author_facet |
Hyeree Choi Sagar B Kudchodkar Emma L Reuschel Kanika Asija Piyush Borole Michelle Ho Krzysztof Wojtak Charles Reed Stephanie Ramos Nathen E Bopp Patricia V Aguilar Scott C Weaver J Joseph Kim Laurent Humeau Pablo Tebas David B Weiner Kar Muthumani |
author_sort |
Hyeree Choi |
title |
Protective immunity by an engineered DNA vaccine for Mayaro virus. |
title_short |
Protective immunity by an engineered DNA vaccine for Mayaro virus. |
title_full |
Protective immunity by an engineered DNA vaccine for Mayaro virus. |
title_fullStr |
Protective immunity by an engineered DNA vaccine for Mayaro virus. |
title_full_unstemmed |
Protective immunity by an engineered DNA vaccine for Mayaro virus. |
title_sort |
protective immunity by an engineered dna vaccine for mayaro virus. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007042 https://doaj.org/article/365111ea733342ab82ae66dc7897d8a4 |
long_lat |
ENVELOPE(-60.734,-60.734,-63.816,-63.816) |
geographic |
Arctic Trinidad |
geographic_facet |
Arctic Trinidad |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007042 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007042 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007042 https://doaj.org/article/365111ea733342ab82ae66dc7897d8a4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007042 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
2 |
container_start_page |
e0007042 |
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1766338645169537024 |