Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism.
Dengue virus (DENV) is the most important vector-borne virus globally. The safe and effective vaccines are still under development and there are no antiviral drugs for DENV induced diseases. In this study, we obtained five DENV1 isolates (DENV1 A to E) from the outbreak of dengue fever in 2014 of Gu...
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ftdoajarticles:oai:doaj.org/article:36495a146f424b9183138b7145511f73 2023-05-15T15:05:41+02:00 Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. Chunling Zou Chenxiao Huang Jinyu Zhang Qihan Wu Xiaohua Ni Jiufeng Sun Jianfeng Dai 2019-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007202 https://doaj.org/article/36495a146f424b9183138b7145511f73 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6417740?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007202 https://doaj.org/article/36495a146f424b9183138b7145511f73 PLoS Neglected Tropical Diseases, Vol 13, Iss 3, p e0007202 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007202 2022-12-31T01:58:21Z Dengue virus (DENV) is the most important vector-borne virus globally. The safe and effective vaccines are still under development and there are no antiviral drugs for DENV induced diseases. In this study, we obtained five DENV1 isolates (DENV1 A to E) from the outbreak of dengue fever in 2014 of Guangzhou, China, and analyzed their replication efficiency and virulence in vitro and in vivo. The results suggested that among the five DENV1 strains, DENV1 B has the highest replication efficiency in both human and mosquito cells in vitro, also causes the highest mortality to suckling mice. Further study suggested that nonstructural proteins from DENV1B have higher capacity to suppress host interferon signaling. In addition, the NS2B3 protease from DENV1B has higher enzymatic activity compared with that from DENV1 E. Finally, we identified that the 64th amino acid of NS2A and the 55th amino acid of NS2B were two virulence determining sites for DENV1. This study provided new evidences of the molecular mechanisms of DENV virulence. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 3 e0007202 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Chunling Zou Chenxiao Huang Jinyu Zhang Qihan Wu Xiaohua Ni Jiufeng Sun Jianfeng Dai Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Dengue virus (DENV) is the most important vector-borne virus globally. The safe and effective vaccines are still under development and there are no antiviral drugs for DENV induced diseases. In this study, we obtained five DENV1 isolates (DENV1 A to E) from the outbreak of dengue fever in 2014 of Guangzhou, China, and analyzed their replication efficiency and virulence in vitro and in vivo. The results suggested that among the five DENV1 strains, DENV1 B has the highest replication efficiency in both human and mosquito cells in vitro, also causes the highest mortality to suckling mice. Further study suggested that nonstructural proteins from DENV1B have higher capacity to suppress host interferon signaling. In addition, the NS2B3 protease from DENV1B has higher enzymatic activity compared with that from DENV1 E. Finally, we identified that the 64th amino acid of NS2A and the 55th amino acid of NS2B were two virulence determining sites for DENV1. This study provided new evidences of the molecular mechanisms of DENV virulence. |
format |
Article in Journal/Newspaper |
author |
Chunling Zou Chenxiao Huang Jinyu Zhang Qihan Wu Xiaohua Ni Jiufeng Sun Jianfeng Dai |
author_facet |
Chunling Zou Chenxiao Huang Jinyu Zhang Qihan Wu Xiaohua Ni Jiufeng Sun Jianfeng Dai |
author_sort |
Chunling Zou |
title |
Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. |
title_short |
Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. |
title_full |
Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. |
title_fullStr |
Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. |
title_full_unstemmed |
Virulence difference of five type I dengue viruses and the intrinsic molecular mechanism. |
title_sort |
virulence difference of five type i dengue viruses and the intrinsic molecular mechanism. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007202 https://doaj.org/article/36495a146f424b9183138b7145511f73 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 3, p e0007202 (2019) |
op_relation |
http://europepmc.org/articles/PMC6417740?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007202 https://doaj.org/article/36495a146f424b9183138b7145511f73 |
op_doi |
https://doi.org/10.1371/journal.pntd.0007202 |
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PLOS Neglected Tropical Diseases |
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13 |
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3 |
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e0007202 |
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