Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847)
Objective: To investigate the parasitic infection of Anentome helena (A. helena) and determine the validity of species boundaries for A. helena by combining molecular phylogeny and morphological approaches. Methods: A total of 325 individuals of A. helena were collected throughout northern Thailand....
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:36028a1c6ba04281ad13c7716807c314 2023-05-15T15:10:29+02:00 Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847) Nithinan Chomchoei Chalobol Wongsawad Nattawadee Nantarat 2018-01-01T00:00:00Z https://doi.org/10.4103/1995-7645.244524 https://doaj.org/article/36028a1c6ba04281ad13c7716807c314 EN eng Wolters Kluwer Medknow Publications http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=10;spage=590;epage=596;aulast=Chomchoei https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.244524 https://doaj.org/article/36028a1c6ba04281ad13c7716807c314 Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 10, Pp 590-596 (2018) phylogeny freshwater snail echinostomes biodiversity anentome Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.4103/1995-7645.244524 2022-12-31T01:46:03Z Objective: To investigate the parasitic infection of Anentome helena (A. helena) and determine the validity of species boundaries for A. helena by combining molecular phylogeny and morphological approaches. Methods: A total of 325 individuals of A. helena were collected throughout northern Thailand. Shells were measured and compared by t-test. Radulae were investigated by using light and scanning electron microscope. Two partial mitochondrial DNA sequences of COI and 16S rRNA from 36 specimens of A. helena and related species were used to test the validity of the morphospecies. Phylogenetic trees were constructed using neighbour joining, maximum likelihood and Bayesian inference. Infection of A. helena with trematode larva was examined and observed. Results: Morphological examination of A. helena revealed 2 distinct morphospecies. Genetic divergences supported the separation of the two morphotypes into two distinct groups. Both individual and combined analyses of the two nucleotide fragments revealed two phylogroups that corresponded with shell and radula characteristics. In addition, A. helena was found infected with 37-collar spined echinostome metacercariae. The prevalence and intensity of metacercariae was highest in the San Kamphaeng district, Chiang Mai province, with 7.5% and 1.670±0.577, respectively. Conclusions: These findings suggest that comprehensive taxonomic revision of this unrecognised species complex is needed. This study represents the preliminary step to reveal new data on the recent distribution of trematode infection in A. helena. This information may be useful for developing conservation management of the snail and the practice of targeted regimes to reduce anthelmintic resistance in the future. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Chiang ENVELOPE(162.650,162.650,-77.967,-77.967) Asian Pacific Journal of Tropical Medicine 11 10 590 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
phylogeny freshwater snail echinostomes biodiversity anentome Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
phylogeny freshwater snail echinostomes biodiversity anentome Arctic medicine. Tropical medicine RC955-962 Nithinan Chomchoei Chalobol Wongsawad Nattawadee Nantarat Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847) |
topic_facet |
phylogeny freshwater snail echinostomes biodiversity anentome Arctic medicine. Tropical medicine RC955-962 |
description |
Objective: To investigate the parasitic infection of Anentome helena (A. helena) and determine the validity of species boundaries for A. helena by combining molecular phylogeny and morphological approaches. Methods: A total of 325 individuals of A. helena were collected throughout northern Thailand. Shells were measured and compared by t-test. Radulae were investigated by using light and scanning electron microscope. Two partial mitochondrial DNA sequences of COI and 16S rRNA from 36 specimens of A. helena and related species were used to test the validity of the morphospecies. Phylogenetic trees were constructed using neighbour joining, maximum likelihood and Bayesian inference. Infection of A. helena with trematode larva was examined and observed. Results: Morphological examination of A. helena revealed 2 distinct morphospecies. Genetic divergences supported the separation of the two morphotypes into two distinct groups. Both individual and combined analyses of the two nucleotide fragments revealed two phylogroups that corresponded with shell and radula characteristics. In addition, A. helena was found infected with 37-collar spined echinostome metacercariae. The prevalence and intensity of metacercariae was highest in the San Kamphaeng district, Chiang Mai province, with 7.5% and 1.670±0.577, respectively. Conclusions: These findings suggest that comprehensive taxonomic revision of this unrecognised species complex is needed. This study represents the preliminary step to reveal new data on the recent distribution of trematode infection in A. helena. This information may be useful for developing conservation management of the snail and the practice of targeted regimes to reduce anthelmintic resistance in the future. |
format |
Article in Journal/Newspaper |
author |
Nithinan Chomchoei Chalobol Wongsawad Nattawadee Nantarat |
author_facet |
Nithinan Chomchoei Chalobol Wongsawad Nattawadee Nantarat |
author_sort |
Nithinan Chomchoei |
title |
Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847) |
title_short |
Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847) |
title_full |
Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847) |
title_fullStr |
Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847) |
title_full_unstemmed |
Investigation of cryptic diversity and occurrence of echinostome metacercariae infection in Anentome helena (von dem Busch, 1847) |
title_sort |
investigation of cryptic diversity and occurrence of echinostome metacercariae infection in anentome helena (von dem busch, 1847) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2018 |
url |
https://doi.org/10.4103/1995-7645.244524 https://doaj.org/article/36028a1c6ba04281ad13c7716807c314 |
long_lat |
ENVELOPE(162.650,162.650,-77.967,-77.967) |
geographic |
Arctic Chiang |
geographic_facet |
Arctic Chiang |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Medicine, Vol 11, Iss 10, Pp 590-596 (2018) |
op_relation |
http://www.apjtm.org/article.asp?issn=1995-7645;year=2018;volume=11;issue=10;spage=590;epage=596;aulast=Chomchoei https://doaj.org/toc/2352-4146 2352-4146 doi:10.4103/1995-7645.244524 https://doaj.org/article/36028a1c6ba04281ad13c7716807c314 |
op_doi |
https://doi.org/10.4103/1995-7645.244524 |
container_title |
Asian Pacific Journal of Tropical Medicine |
container_volume |
11 |
container_issue |
10 |
container_start_page |
590 |
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1766341509035065344 |