The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages.
Toxocariasis is a neglected parasitic disease caused predominantly by larvae of Toxocara canis. While this zoonotic disease is of major importance in humans and canids, it can also affect a range of other mammalian hosts. It is known that mucins secreted by larvae play key roles in immune recognitio...
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ftdoajarticles:oai:doaj.org/article:35fd690b1bf64c0483ec1c0d930e1cbb 2023-05-15T15:10:30+02:00 The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages. Rongqiong Zhou Hongguo Jia Zhendong Du Aiyun Jiang Zhenhui Song Tao Wang Aifang Du Robin B Gasser Guangxu Ma 2022-09-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010734 https://doaj.org/article/35fd690b1bf64c0483ec1c0d930e1cbb EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010734 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010734 https://doaj.org/article/35fd690b1bf64c0483ec1c0d930e1cbb PLoS Neglected Tropical Diseases, Vol 16, Iss 9, p e0010734 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010734 2022-12-30T19:57:24Z Toxocariasis is a neglected parasitic disease caused predominantly by larvae of Toxocara canis. While this zoonotic disease is of major importance in humans and canids, it can also affect a range of other mammalian hosts. It is known that mucins secreted by larvae play key roles in immune recognition and evasion, but very little is understood about the molecular interactions between host cells and T. canis. Here, using an integrative approach (affinity pull-down, mass spectrometry, co-immunoprecipitation and bioinformatics), we identified 219 proteins expressed by a murine macrophage cell line (RAW264.7) that interact with prokaryotically-expressed recombinant protein (rTc-MUC-1) representing the mucin Tc-MUC-1 present in the surface coat of infective larvae of T. canis. Protein-protein interactions between rTc-MUC-1 and an actin binding protein CFL1 as well as the fatty acid binding protein FABP5 of RAW264.7 macrophages were also demonstrated in a human embryonic kidney cell line (HEK 293T). By combing predicted structural information on the protein-protein interaction and functional knowledge of the related protein association networks, we inferred roles for Tc-MUC-1 protein in the regulation of actin cytoskeletal remodelling, and the migration and phagosome formation of macrophage cells. These molecular interactions now require verification in vivo. The experimental approach taken here should be readily applicable to comparative studies of other ascaridoid nematodes (e.g. T. cati, Anisakis simplex, Ascaris suum and Baylisascaris procyonis) whose larvae undergo tissue migration in accidental hosts, including humans. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 9 e0010734 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Rongqiong Zhou Hongguo Jia Zhendong Du Aiyun Jiang Zhenhui Song Tao Wang Aifang Du Robin B Gasser Guangxu Ma The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Toxocariasis is a neglected parasitic disease caused predominantly by larvae of Toxocara canis. While this zoonotic disease is of major importance in humans and canids, it can also affect a range of other mammalian hosts. It is known that mucins secreted by larvae play key roles in immune recognition and evasion, but very little is understood about the molecular interactions between host cells and T. canis. Here, using an integrative approach (affinity pull-down, mass spectrometry, co-immunoprecipitation and bioinformatics), we identified 219 proteins expressed by a murine macrophage cell line (RAW264.7) that interact with prokaryotically-expressed recombinant protein (rTc-MUC-1) representing the mucin Tc-MUC-1 present in the surface coat of infective larvae of T. canis. Protein-protein interactions between rTc-MUC-1 and an actin binding protein CFL1 as well as the fatty acid binding protein FABP5 of RAW264.7 macrophages were also demonstrated in a human embryonic kidney cell line (HEK 293T). By combing predicted structural information on the protein-protein interaction and functional knowledge of the related protein association networks, we inferred roles for Tc-MUC-1 protein in the regulation of actin cytoskeletal remodelling, and the migration and phagosome formation of macrophage cells. These molecular interactions now require verification in vivo. The experimental approach taken here should be readily applicable to comparative studies of other ascaridoid nematodes (e.g. T. cati, Anisakis simplex, Ascaris suum and Baylisascaris procyonis) whose larvae undergo tissue migration in accidental hosts, including humans. |
format |
Article in Journal/Newspaper |
author |
Rongqiong Zhou Hongguo Jia Zhendong Du Aiyun Jiang Zhenhui Song Tao Wang Aifang Du Robin B Gasser Guangxu Ma |
author_facet |
Rongqiong Zhou Hongguo Jia Zhendong Du Aiyun Jiang Zhenhui Song Tao Wang Aifang Du Robin B Gasser Guangxu Ma |
author_sort |
Rongqiong Zhou |
title |
The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages. |
title_short |
The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages. |
title_full |
The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages. |
title_fullStr |
The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages. |
title_full_unstemmed |
The non-glycosylated protein of Toxocara canis MUC-1 interacts with proteins of murine macrophages. |
title_sort |
non-glycosylated protein of toxocara canis muc-1 interacts with proteins of murine macrophages. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010734 https://doaj.org/article/35fd690b1bf64c0483ec1c0d930e1cbb |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 9, p e0010734 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010734 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010734 https://doaj.org/article/35fd690b1bf64c0483ec1c0d930e1cbb |
op_doi |
https://doi.org/10.1371/journal.pntd.0010734 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
9 |
container_start_page |
e0010734 |
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1766341519796600832 |