Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge
The Sea of Okhotsk is known as one of the most biologically productive regions among the world's oceans, and its productivity is supported in part by the discharge of iron (Fe)-rich water from the Amur River. However, little is known about the effect of riverine-derived Fe input on the physiolo...
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2014
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ftdoajarticles:oai:doaj.org/article:35c9938711a4420ab9ffea264a1474b3 2023-05-15T18:09:08+02:00 Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge K. Suzuki A. Hattori-Saito Y. Sekiguchi J. Nishioka M. Shigemitsu T. Isada H. Liu R. M. L. McKay 2014-05-01T00:00:00Z https://doi.org/10.5194/bg-11-2503-2014 https://doaj.org/article/35c9938711a4420ab9ffea264a1474b3 EN eng Copernicus Publications http://www.biogeosciences.net/11/2503/2014/bg-11-2503-2014.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-11-2503-2014 https://doaj.org/article/35c9938711a4420ab9ffea264a1474b3 Biogeosciences, Vol 11, Iss 9, Pp 2503-2517 (2014) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2014 ftdoajarticles https://doi.org/10.5194/bg-11-2503-2014 2022-12-31T14:57:45Z The Sea of Okhotsk is known as one of the most biologically productive regions among the world's oceans, and its productivity is supported in part by the discharge of iron (Fe)-rich water from the Amur River. However, little is known about the effect of riverine-derived Fe input on the physiology of the large diatoms which often flourish in surface waters of the productive continental shelf region. We conducted diatom-specific immunochemical ferredoxin (Fd) and flavodoxin (Fld) assays in order to investigate the spatial variability of Fe nutritional status in the microplankton-sized (20–200 μm; hereafter micro-sized) diatoms. The Fd index, defined as the proportion of Fd to the sum of Fd plus Fld accumulations in the cells, was used to assess their Fe nutritional status. Additionally, active chlorophyll fluorescence measurements using pulse–amplitude-modulated (PAM) fluorometry were carried out to obtain the maximum photochemical quantum efficiency ( F v / F m ) of photosystem II for the total micro-sized phytoplankton assemblages including diatoms. During our observations in the summer of 2006, the micro-sized diatoms were relatively abundant (> 10 μg C L −1 ) in the neritic region, and formed a massive bloom in Sakhalin Bay near the mouth of the Amur River. Values of the Fd index and F v / F m were high (>0.9 and >0.65, respectively) near the river mouth, indicating that Fe was sufficient for growth of the diatoms. However, in oceanic waters of the Sea of Okhotsk, the diatom Fd index declined as cellular Fld accumulation increased. These results suggest that there was a distinct gradient in Fe nutritional status in the micro-sized diatoms from near the Amur River mouth to open waters in the Sea of Okhotsk. A significant correlation between dissolved Fe (D-Fe) concentration and the Fd index was found in waters off Sakhalin Island, indicating that D-Fe was a key factor for the photophysiology of this diatom size class. In the vicinity of the Kuril Islands between the Sea of Okhotsk and the Pacific ... Article in Journal/Newspaper Sakhalin Directory of Open Access Journals: DOAJ Articles Okhotsk Pacific Biogeosciences 11 9 2503 2517 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 K. Suzuki A. Hattori-Saito Y. Sekiguchi J. Nishioka M. Shigemitsu T. Isada H. Liu R. M. L. McKay Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
The Sea of Okhotsk is known as one of the most biologically productive regions among the world's oceans, and its productivity is supported in part by the discharge of iron (Fe)-rich water from the Amur River. However, little is known about the effect of riverine-derived Fe input on the physiology of the large diatoms which often flourish in surface waters of the productive continental shelf region. We conducted diatom-specific immunochemical ferredoxin (Fd) and flavodoxin (Fld) assays in order to investigate the spatial variability of Fe nutritional status in the microplankton-sized (20–200 μm; hereafter micro-sized) diatoms. The Fd index, defined as the proportion of Fd to the sum of Fd plus Fld accumulations in the cells, was used to assess their Fe nutritional status. Additionally, active chlorophyll fluorescence measurements using pulse–amplitude-modulated (PAM) fluorometry were carried out to obtain the maximum photochemical quantum efficiency ( F v / F m ) of photosystem II for the total micro-sized phytoplankton assemblages including diatoms. During our observations in the summer of 2006, the micro-sized diatoms were relatively abundant (> 10 μg C L −1 ) in the neritic region, and formed a massive bloom in Sakhalin Bay near the mouth of the Amur River. Values of the Fd index and F v / F m were high (>0.9 and >0.65, respectively) near the river mouth, indicating that Fe was sufficient for growth of the diatoms. However, in oceanic waters of the Sea of Okhotsk, the diatom Fd index declined as cellular Fld accumulation increased. These results suggest that there was a distinct gradient in Fe nutritional status in the micro-sized diatoms from near the Amur River mouth to open waters in the Sea of Okhotsk. A significant correlation between dissolved Fe (D-Fe) concentration and the Fd index was found in waters off Sakhalin Island, indicating that D-Fe was a key factor for the photophysiology of this diatom size class. In the vicinity of the Kuril Islands between the Sea of Okhotsk and the Pacific ... |
format |
Article in Journal/Newspaper |
author |
K. Suzuki A. Hattori-Saito Y. Sekiguchi J. Nishioka M. Shigemitsu T. Isada H. Liu R. M. L. McKay |
author_facet |
K. Suzuki A. Hattori-Saito Y. Sekiguchi J. Nishioka M. Shigemitsu T. Isada H. Liu R. M. L. McKay |
author_sort |
K. Suzuki |
title |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_short |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_full |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_fullStr |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_full_unstemmed |
Spatial variability in iron nutritional status of large diatoms in the Sea of Okhotsk with special reference to the Amur River discharge |
title_sort |
spatial variability in iron nutritional status of large diatoms in the sea of okhotsk with special reference to the amur river discharge |
publisher |
Copernicus Publications |
publishDate |
2014 |
url |
https://doi.org/10.5194/bg-11-2503-2014 https://doaj.org/article/35c9938711a4420ab9ffea264a1474b3 |
geographic |
Okhotsk Pacific |
geographic_facet |
Okhotsk Pacific |
genre |
Sakhalin |
genre_facet |
Sakhalin |
op_source |
Biogeosciences, Vol 11, Iss 9, Pp 2503-2517 (2014) |
op_relation |
http://www.biogeosciences.net/11/2503/2014/bg-11-2503-2014.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-11-2503-2014 https://doaj.org/article/35c9938711a4420ab9ffea264a1474b3 |
op_doi |
https://doi.org/10.5194/bg-11-2503-2014 |
container_title |
Biogeosciences |
container_volume |
11 |
container_issue |
9 |
container_start_page |
2503 |
op_container_end_page |
2517 |
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1766181556201717760 |