Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives
Abstract Background The small, single-stranded positive-sense RNA astroviruses are mostly known to be enteric viruses. In recent years, though, different astroviruses were reported in association with neurological disease in various species. In cattle, two distinct neurotropic astrovirus genotype sp...
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ftdoajarticles:oai:doaj.org/article:35ae4be9c1c9452da1c2730f05ed53b0 2023-05-15T17:13:40+02:00 Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives Céline Louise Boujon Michel Christoph Koch Ronja Véronique Kauer Elsbeth Keller-Gautschi Melanie Michaela Hierweger Stefan Hoby Torsten Seuberlich 2019-06-01T00:00:00Z https://doi.org/10.1186/s13028-019-0466-0 https://doaj.org/article/35ae4be9c1c9452da1c2730f05ed53b0 EN eng BMC http://link.springer.com/article/10.1186/s13028-019-0466-0 https://doaj.org/toc/1751-0147 doi:10.1186/s13028-019-0466-0 1751-0147 https://doaj.org/article/35ae4be9c1c9452da1c2730f05ed53b0 Acta Veterinaria Scandinavica, Vol 61, Iss 1, Pp 1-9 (2019) Astrovirus Encephalitis Formalin-fixed and paraffin embedded (FFPE) Muskox (Ovibos moschatus) Next-generation sequencing (NGS) Veterinary medicine SF600-1100 article 2019 ftdoajarticles https://doi.org/10.1186/s13028-019-0466-0 2022-12-31T12:26:50Z Abstract Background The small, single-stranded positive-sense RNA astroviruses are mostly known to be enteric viruses. In recent years, though, different astroviruses were reported in association with neurological disease in various species. In cattle, two distinct neurotropic astrovirus genotype species were described in numerous cases of nonsuppurative encephalomyelitis, with one of these viruses also reported in similar circumstances in several sheep. Here, we retrieved archived formalin-fixed, paraffin-embedded brain tissues of a muskox diagnosed with a comparable disease pattern in 1982 and investigated them for the presence of neurotropic astroviruses with various techniques. Results Initially, tissue samples scored positive for both neurotropic astroviruses by immunohistochemistry; however, unexpected results with further immunohistochemical testing, in situ hybridization and qRT-PCR prompted us to submit an RNA extract from the animal’s brain material to next-generation sequencing. We were thus able to obtain the full genome of a novel astrovirus, muskox astrovirus CH18 (MOxAstV-CH18), whose closest relative is an enteric ovine astrovirus. Subsequently, viral RNA could be detected with a specific RT-PCR in the brain of the affected animal, but not in faecal samples from the current muskoxen herd of the animal park where the animal used to be kept. Conclusions We identified a novel astrovirus in a historical case of a captive muskox with nonsuppurative encephalomyelitis. Unfortunately, our results and the fact that no material from organs other than of the nervous system was available do not allow any assumption about the epidemiology or pathogenesis of the virus. Still, these findings are yet another piece of evidence that the tropism and species specificity of astroviruses could be more deceptive than generally assumed. Article in Journal/Newspaper muskox ovibos moschatus Directory of Open Access Journals: DOAJ Articles Acta Veterinaria Scandinavica 61 1 |
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topic |
Astrovirus Encephalitis Formalin-fixed and paraffin embedded (FFPE) Muskox (Ovibos moschatus) Next-generation sequencing (NGS) Veterinary medicine SF600-1100 |
spellingShingle |
Astrovirus Encephalitis Formalin-fixed and paraffin embedded (FFPE) Muskox (Ovibos moschatus) Next-generation sequencing (NGS) Veterinary medicine SF600-1100 Céline Louise Boujon Michel Christoph Koch Ronja Véronique Kauer Elsbeth Keller-Gautschi Melanie Michaela Hierweger Stefan Hoby Torsten Seuberlich Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives |
topic_facet |
Astrovirus Encephalitis Formalin-fixed and paraffin embedded (FFPE) Muskox (Ovibos moschatus) Next-generation sequencing (NGS) Veterinary medicine SF600-1100 |
description |
Abstract Background The small, single-stranded positive-sense RNA astroviruses are mostly known to be enteric viruses. In recent years, though, different astroviruses were reported in association with neurological disease in various species. In cattle, two distinct neurotropic astrovirus genotype species were described in numerous cases of nonsuppurative encephalomyelitis, with one of these viruses also reported in similar circumstances in several sheep. Here, we retrieved archived formalin-fixed, paraffin-embedded brain tissues of a muskox diagnosed with a comparable disease pattern in 1982 and investigated them for the presence of neurotropic astroviruses with various techniques. Results Initially, tissue samples scored positive for both neurotropic astroviruses by immunohistochemistry; however, unexpected results with further immunohistochemical testing, in situ hybridization and qRT-PCR prompted us to submit an RNA extract from the animal’s brain material to next-generation sequencing. We were thus able to obtain the full genome of a novel astrovirus, muskox astrovirus CH18 (MOxAstV-CH18), whose closest relative is an enteric ovine astrovirus. Subsequently, viral RNA could be detected with a specific RT-PCR in the brain of the affected animal, but not in faecal samples from the current muskoxen herd of the animal park where the animal used to be kept. Conclusions We identified a novel astrovirus in a historical case of a captive muskox with nonsuppurative encephalomyelitis. Unfortunately, our results and the fact that no material from organs other than of the nervous system was available do not allow any assumption about the epidemiology or pathogenesis of the virus. Still, these findings are yet another piece of evidence that the tropism and species specificity of astroviruses could be more deceptive than generally assumed. |
format |
Article in Journal/Newspaper |
author |
Céline Louise Boujon Michel Christoph Koch Ronja Véronique Kauer Elsbeth Keller-Gautschi Melanie Michaela Hierweger Stefan Hoby Torsten Seuberlich |
author_facet |
Céline Louise Boujon Michel Christoph Koch Ronja Véronique Kauer Elsbeth Keller-Gautschi Melanie Michaela Hierweger Stefan Hoby Torsten Seuberlich |
author_sort |
Céline Louise Boujon |
title |
Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives |
title_short |
Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives |
title_full |
Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives |
title_fullStr |
Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives |
title_full_unstemmed |
Novel encephalomyelitis-associated astrovirus in a muskox (Ovibos moschatus): a surprise from the archives |
title_sort |
novel encephalomyelitis-associated astrovirus in a muskox (ovibos moschatus): a surprise from the archives |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s13028-019-0466-0 https://doaj.org/article/35ae4be9c1c9452da1c2730f05ed53b0 |
genre |
muskox ovibos moschatus |
genre_facet |
muskox ovibos moschatus |
op_source |
Acta Veterinaria Scandinavica, Vol 61, Iss 1, Pp 1-9 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s13028-019-0466-0 https://doaj.org/toc/1751-0147 doi:10.1186/s13028-019-0466-0 1751-0147 https://doaj.org/article/35ae4be9c1c9452da1c2730f05ed53b0 |
op_doi |
https://doi.org/10.1186/s13028-019-0466-0 |
container_title |
Acta Veterinaria Scandinavica |
container_volume |
61 |
container_issue |
1 |
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1766070839159029760 |