Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia
Introduction: A total of 192 invasive Streptococcus pneumoniae isolates, from serotypes 11A, 15B/C and 23A (not included in the conjugated vaccines), were collected in Colombia between 1994 and 2014 as part of the activities of the Network surveillance system for the causative agents of pneumonia an...
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ftdoajarticles:oai:doaj.org/article:358c22af3d4940a3bdc67cabf4dfd6a7 2023-05-15T15:08:18+02:00 Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia Paola Andrea Palacios Carolina Duarte Olga Sanabria Jaime Moreno 2017-09-01T00:00:00Z https://doi.org/10.7705/biomedica.v37i3.3223 https://doaj.org/article/358c22af3d4940a3bdc67cabf4dfd6a7 EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3223 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i3.3223 https://doaj.org/article/358c22af3d4940a3bdc67cabf4dfd6a7 Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 3, Pp 390-396 (2017) Vaccines conjugate pneumonia meningitis pneumococcal infections multilocus sequence typing electrophoresis gel pulsed-field Medicine R Arctic medicine. Tropical medicine RC955-962 article 2017 ftdoajarticles https://doi.org/10.7705/biomedica.v37i3.3223 2022-12-31T00:46:54Z Introduction: A total of 192 invasive Streptococcus pneumoniae isolates, from serotypes 11A, 15B/C and 23A (not included in the conjugated vaccines), were collected in Colombia between 1994 and 2014 as part of the activities of the Network surveillance system for the causative agents of pneumonia and meningitis (SIREVA II). Objective: To determine the molecular characteristics of invasive S. pneumoniae isolates from serotypes 11A, 15B/C and 23A in Colombia from 1994 to 2014. Materials and methods: The molecular characterization of the isolates was carried out through Pulse-Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST). Results: Serotype 11A showed one clonal group represented by ST62. Serotype 15B/C was composed of three groups associated with Netherlands15B-37 ST199 (28.75%), ST8495 (18.75%), and SLV (Single-Locus Variant) of ST193 (21.25%). Isolates from serotype 23A were gathered in three clonal groups, with 70.21% closely related to ST42, 17.02% to Colombia23F-ST338, and 6.38% to Netherlands15B-37 ST199. Conclusion: Clones Colombia23F-ST338 and Netherlands15B-ST199 covered more serotypes than those previously found by other authors, including serotype 23A. These analyses reveal the importance of capsular switching in the spreading of successful clones among non-vaccine serotypes causing invasive pneumococcal disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 37 3 390 |
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Vaccines conjugate pneumonia meningitis pneumococcal infections multilocus sequence typing electrophoresis gel pulsed-field Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Vaccines conjugate pneumonia meningitis pneumococcal infections multilocus sequence typing electrophoresis gel pulsed-field Medicine R Arctic medicine. Tropical medicine RC955-962 Paola Andrea Palacios Carolina Duarte Olga Sanabria Jaime Moreno Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia |
topic_facet |
Vaccines conjugate pneumonia meningitis pneumococcal infections multilocus sequence typing electrophoresis gel pulsed-field Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: A total of 192 invasive Streptococcus pneumoniae isolates, from serotypes 11A, 15B/C and 23A (not included in the conjugated vaccines), were collected in Colombia between 1994 and 2014 as part of the activities of the Network surveillance system for the causative agents of pneumonia and meningitis (SIREVA II). Objective: To determine the molecular characteristics of invasive S. pneumoniae isolates from serotypes 11A, 15B/C and 23A in Colombia from 1994 to 2014. Materials and methods: The molecular characterization of the isolates was carried out through Pulse-Field Gel Electrophoresis (PFGE) and Multilocus Sequence Typing (MLST). Results: Serotype 11A showed one clonal group represented by ST62. Serotype 15B/C was composed of three groups associated with Netherlands15B-37 ST199 (28.75%), ST8495 (18.75%), and SLV (Single-Locus Variant) of ST193 (21.25%). Isolates from serotype 23A were gathered in three clonal groups, with 70.21% closely related to ST42, 17.02% to Colombia23F-ST338, and 6.38% to Netherlands15B-37 ST199. Conclusion: Clones Colombia23F-ST338 and Netherlands15B-ST199 covered more serotypes than those previously found by other authors, including serotype 23A. These analyses reveal the importance of capsular switching in the spreading of successful clones among non-vaccine serotypes causing invasive pneumococcal disease. |
format |
Article in Journal/Newspaper |
author |
Paola Andrea Palacios Carolina Duarte Olga Sanabria Jaime Moreno |
author_facet |
Paola Andrea Palacios Carolina Duarte Olga Sanabria Jaime Moreno |
author_sort |
Paola Andrea Palacios |
title |
Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia |
title_short |
Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia |
title_full |
Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia |
title_fullStr |
Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia |
title_full_unstemmed |
Molecular characterization of non-vaccine Streptococcus pneumoniae serotypes 11A, 15 B/C and 23A recovered from invasive isolates in Colombia |
title_sort |
molecular characterization of non-vaccine streptococcus pneumoniae serotypes 11a, 15 b/c and 23a recovered from invasive isolates in colombia |
publisher |
Instituto Nacional de Salud |
publishDate |
2017 |
url |
https://doi.org/10.7705/biomedica.v37i3.3223 https://doaj.org/article/358c22af3d4940a3bdc67cabf4dfd6a7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 37, Iss 3, Pp 390-396 (2017) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3223 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v37i3.3223 https://doaj.org/article/358c22af3d4940a3bdc67cabf4dfd6a7 |
op_doi |
https://doi.org/10.7705/biomedica.v37i3.3223 |
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Biomédica |
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37 |
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390 |
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