The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia
Introduction. Pneumocystis jirovecii is an opportunistic fungus that affects mainly people living with HIV (CD4 cell count lower than 200 cells/ml) and other immunosuppressed patients. Since P. jirovecii does not grow on routine mycological media, diagnosis of P. jirovecii pneumonia relies on indire...
Published in: | Biomédica |
---|---|
Main Authors: | , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English Spanish |
Published: |
Instituto Nacional de Salud
2023
|
Subjects: | |
Online Access: | https://doi.org/10.7705/biomedica.7020 https://doaj.org/article/35517f35513d459e9af03adb6731d045 |
id |
ftdoajarticles:oai:doaj.org/article:35517f35513d459e9af03adb6731d045 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:35517f35513d459e9af03adb6731d045 2023-10-09T21:49:12+02:00 The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia Fernando Almeida-Siva Rodrigo Almeida-Paes Lisandra Serra-Damasceno Edwiges Motta-Santos Luiz Claudio Ferreira Leonardo Pereira-Quintella Marcela de Faria Ferreira Mauro de Medeiros-Muniz Rosely M. Zancopé-Oliveira 2023-08-01T00:00:00Z https://doi.org/10.7705/biomedica.7020 https://doaj.org/article/35517f35513d459e9af03adb6731d045 EN ES eng spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/7020 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.7020 https://doaj.org/article/35517f35513d459e9af03adb6731d045 Biomédica: revista del Instituto Nacional de Salud, Vol 43, Iss Sp. 1, Pp 255-266 (2023) pneumonia pneumocystis fluorescent antibody technique direct real-time polymerase chain reaction Medicine R Arctic medicine. Tropical medicine RC955-962 article 2023 ftdoajarticles https://doi.org/10.7705/biomedica.7020 2023-09-24T00:42:21Z Introduction. Pneumocystis jirovecii is an opportunistic fungus that affects mainly people living with HIV (CD4 cell count lower than 200 cells/ml) and other immunosuppressed patients. Since P. jirovecii does not grow on routine mycological media, diagnosis of P. jirovecii pneumonia relies on indirect evidence of its presence in respiratory samples. Objectives. To associate the results of direct immunofluorescence and two molecular methods with a score to predict P. jirovecii pneumonia in patients with AIDS. Materials and methods. A prospective study was conducted with 40 patients. A respiratory sample collected before treatment was subjected to direct immunofluorescence using the Merifluor kit, to nested PCR targeting the mitochondrial large subunit ribosomal RNA, and to the VIASURE real-time PCR kit. Results. These three techniques revealed P. jirovecii in 6, 12, and 15 samples, respectively. All positive samples by direct immunofluorescence were positive by nested PCR, and all positive samples by nested PCR amplified by real-time PCR. There was a statistically significant association between the P. jirovecii pneumonia score and the molecular methods. Two patients were early diagnosed and responded well to treatment. Conclusion. Molecular methods, especially real-time PCR, are recommended for early diagnosis of P. jirovecii pneumonia in AIDS patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 43 Sp. 1 255 266 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Spanish |
topic |
pneumonia pneumocystis fluorescent antibody technique direct real-time polymerase chain reaction Medicine R Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
pneumonia pneumocystis fluorescent antibody technique direct real-time polymerase chain reaction Medicine R Arctic medicine. Tropical medicine RC955-962 Fernando Almeida-Siva Rodrigo Almeida-Paes Lisandra Serra-Damasceno Edwiges Motta-Santos Luiz Claudio Ferreira Leonardo Pereira-Quintella Marcela de Faria Ferreira Mauro de Medeiros-Muniz Rosely M. Zancopé-Oliveira The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia |
topic_facet |
pneumonia pneumocystis fluorescent antibody technique direct real-time polymerase chain reaction Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction. Pneumocystis jirovecii is an opportunistic fungus that affects mainly people living with HIV (CD4 cell count lower than 200 cells/ml) and other immunosuppressed patients. Since P. jirovecii does not grow on routine mycological media, diagnosis of P. jirovecii pneumonia relies on indirect evidence of its presence in respiratory samples. Objectives. To associate the results of direct immunofluorescence and two molecular methods with a score to predict P. jirovecii pneumonia in patients with AIDS. Materials and methods. A prospective study was conducted with 40 patients. A respiratory sample collected before treatment was subjected to direct immunofluorescence using the Merifluor kit, to nested PCR targeting the mitochondrial large subunit ribosomal RNA, and to the VIASURE real-time PCR kit. Results. These three techniques revealed P. jirovecii in 6, 12, and 15 samples, respectively. All positive samples by direct immunofluorescence were positive by nested PCR, and all positive samples by nested PCR amplified by real-time PCR. There was a statistically significant association between the P. jirovecii pneumonia score and the molecular methods. Two patients were early diagnosed and responded well to treatment. Conclusion. Molecular methods, especially real-time PCR, are recommended for early diagnosis of P. jirovecii pneumonia in AIDS patients. |
format |
Article in Journal/Newspaper |
author |
Fernando Almeida-Siva Rodrigo Almeida-Paes Lisandra Serra-Damasceno Edwiges Motta-Santos Luiz Claudio Ferreira Leonardo Pereira-Quintella Marcela de Faria Ferreira Mauro de Medeiros-Muniz Rosely M. Zancopé-Oliveira |
author_facet |
Fernando Almeida-Siva Rodrigo Almeida-Paes Lisandra Serra-Damasceno Edwiges Motta-Santos Luiz Claudio Ferreira Leonardo Pereira-Quintella Marcela de Faria Ferreira Mauro de Medeiros-Muniz Rosely M. Zancopé-Oliveira |
author_sort |
Fernando Almeida-Siva |
title |
The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia |
title_short |
The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia |
title_full |
The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia |
title_fullStr |
The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia |
title_full_unstemmed |
The conventional diagnosis challenge: Real-time PCR and nested PCR correlation with the scoring system for individuals at high-risk of Pneumocystis jirovecii pneumonia |
title_sort |
conventional diagnosis challenge: real-time pcr and nested pcr correlation with the scoring system for individuals at high-risk of pneumocystis jirovecii pneumonia |
publisher |
Instituto Nacional de Salud |
publishDate |
2023 |
url |
https://doi.org/10.7705/biomedica.7020 https://doaj.org/article/35517f35513d459e9af03adb6731d045 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 43, Iss Sp. 1, Pp 255-266 (2023) |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/7020 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.7020 https://doaj.org/article/35517f35513d459e9af03adb6731d045 |
op_doi |
https://doi.org/10.7705/biomedica.7020 |
container_title |
Biomédica |
container_volume |
43 |
container_issue |
Sp. 1 |
container_start_page |
255 |
op_container_end_page |
266 |
_version_ |
1779312224588791808 |