Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom
Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its ve...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:3544e7040d094596a233a4eafebe7493 2023-05-15T15:10:38+02:00 Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom SP Shirmardi M Shamsaei M Gandomkar E Saniei M Ghannadi A Zare 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000400015 https://doaj.org/article/3544e7040d094596a233a4eafebe7493 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400015 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000400015 1678-9199 https://doaj.org/article/3544e7040d094596a233a4eafebe7493 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 4, Pp 639-646 (2010) Mesobuthus eupeus venom purification chromatography radiolabeling biodistribution Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000400015 2022-12-30T23:27:56Z Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin. Finally, toxic subfractions F3.1.6 and F3.1.9 were labeled with 99mTc and injected into normal mice to distinguish excretion pathway. The venom toxic fraction was successfully obtained in its purified form. Radiolabeling of toxic fractions was performed at high specific activity with radiochemical purity of more than 97 and 95% respectively for F3.1.6 and F3.1.9. Biodistribution studies in normal mice with two toxic fractions usually show rapid clearance of the compounds from blood and tissue except for kidneys. Since tissue distribution studies are very important for clinical purpose, the present findings suggest that 99mTc labeling of venom is a useful tool for in vivo studies and comprises an excellent approach to monitoring the process of biodistribution and kinetics of toxins. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 4 639 646 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Mesobuthus eupeus venom purification chromatography radiolabeling biodistribution Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Mesobuthus eupeus venom purification chromatography radiolabeling biodistribution Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 SP Shirmardi M Shamsaei M Gandomkar E Saniei M Ghannadi A Zare Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom |
topic_facet |
Mesobuthus eupeus venom purification chromatography radiolabeling biodistribution Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Iranian scorpions belong mainly to the Buthidae and Scorpionidae families, distributed into 16 genera and 25 species. In Iran, similar to other parts of the world, there are a few known species of scorpions responsible for severe envenoming; amongst which Mesobuthus eupeus is the most common. Its venom contains several toxin fractions that may affect the ion channel. In the present study purification, labeling and biological evaluation of M. eupeus venom are described. For separation, soluble venom was loaded on a chromatography column packed with Sephadex G-50 gel. Subsequently, the fractions were collected according to UV absorption at a wavelength of 280 nm. Toxic fraction (F3) was loaded on an anionic ion exchanger resin and then on a cationic resin. Finally, toxic subfractions F3.1.6 and F3.1.9 were labeled with 99mTc and injected into normal mice to distinguish excretion pathway. The venom toxic fraction was successfully obtained in its purified form. Radiolabeling of toxic fractions was performed at high specific activity with radiochemical purity of more than 97 and 95% respectively for F3.1.6 and F3.1.9. Biodistribution studies in normal mice with two toxic fractions usually show rapid clearance of the compounds from blood and tissue except for kidneys. Since tissue distribution studies are very important for clinical purpose, the present findings suggest that 99mTc labeling of venom is a useful tool for in vivo studies and comprises an excellent approach to monitoring the process of biodistribution and kinetics of toxins. |
format |
Article in Journal/Newspaper |
author |
SP Shirmardi M Shamsaei M Gandomkar E Saniei M Ghannadi A Zare |
author_facet |
SP Shirmardi M Shamsaei M Gandomkar E Saniei M Ghannadi A Zare |
author_sort |
SP Shirmardi |
title |
Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom |
title_short |
Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom |
title_full |
Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom |
title_fullStr |
Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom |
title_full_unstemmed |
Comparison of two purified toxic fractions from Mesobuthus eupeus scorpion venom |
title_sort |
comparison of two purified toxic fractions from mesobuthus eupeus scorpion venom |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000400015 https://doaj.org/article/3544e7040d094596a233a4eafebe7493 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 4, Pp 639-646 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400015 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000400015 1678-9199 https://doaj.org/article/3544e7040d094596a233a4eafebe7493 |
op_doi |
https://doi.org/10.1590/S1678-91992010000400015 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
16 |
container_issue |
4 |
container_start_page |
639 |
op_container_end_page |
646 |
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1766341622086238208 |