Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy
Background. White sturgeon iridovirus (WSIV) is one of the most serious threats for sturgeon aquaculture as the mortality in juvenile individuals can reach 95%. At the turn of the century WSIV was reported among hatchery-raised white sturgeon, Acipenser transmontanus Richardson, 1836, in North Ameri...
Published in: | Acta Ichthyologica et Piscatoria |
---|---|
Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Pensoft Publishers
2019
|
Subjects: | |
Online Access: | https://doi.org/10.3750/AIEP/02568 https://doaj.org/article/3539b47a43974238a7ce6bd35fdb0448 |
id |
ftdoajarticles:oai:doaj.org/article:3539b47a43974238a7ce6bd35fdb0448 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3539b47a43974238a7ce6bd35fdb0448 2023-05-15T13:01:57+02:00 Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy P. Hofsoe-Oppermann J. Kiełpińska R. Panicz S.M. Bergmann 2019-09-01T00:00:00Z https://doi.org/10.3750/AIEP/02568 https://doaj.org/article/3539b47a43974238a7ce6bd35fdb0448 EN eng Pensoft Publishers https://aiep.pensoft.net/article/26906/download/pdf/ https://aiep.pensoft.net/article/26906/download/xml/ https://aiep.pensoft.net/article/26906/ https://doaj.org/toc/1734-1515 doi:10.3750/AIEP/02568 1734-1515 https://doaj.org/article/3539b47a43974238a7ce6bd35fdb0448 Acta Ichthyologica et Piscatoria, Vol 49, Iss 3, Pp 257-264 (2019) white sturgeon iridovirus WSIV aquaculture viru Aquaculture. Fisheries. Angling SH1-691 article 2019 ftdoajarticles https://doi.org/10.3750/AIEP/02568 2022-12-30T21:39:18Z Background. White sturgeon iridovirus (WSIV) is one of the most serious threats for sturgeon aquaculture as the mortality in juvenile individuals can reach 95%. At the turn of the century WSIV was reported among hatchery-raised white sturgeon, Acipenser transmontanus Richardson, 1836, in North America, Russian sturgeon , Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833, in northern Europe but also pallid sturgeon, Scaphirhynchus albus (Forbes et Richardson, 1905) and shovelnose sturgeon, Scaphirhynchus platorynchus (Rafinesque, 1820) in North and South Dakota. The clinical signs of infection include cessation of feeding and edematous and pale gills. Those pathological changes lead to respiratory problems of the juveniles and deterioration of their overall condition. Rapid detection of pathogen particles under aquaculture conditions is crucial, therefore the principal aim of this study was to establish a molecular method for rapid and effective detection of the WSIV. This is the first study thoroughly comparing WSIV detection methods in sturgeons cultured in Poland, Germany, and Italy. Materials and methods. A total of 244 samples were collected from 82 sturgeons representing six species: beluga, Huso huso (Linnaeus, 1758); sterlet, Acipenser ruthenus Linnaeus, 1758; Siberian sturgeon, Acipenser baerii Brandt, 1869; Russian sturgeon, A. gueldenstaedtii; Atlantic sturgeon, Acipenser oxyrinchus Mitchill, 1815; and European sea sturgeon, Acipenser sturio Linnaeus, 1758, as well as five sturgeon hybrids: Siberian × Russian, Adriatic (Acipenser naccarii Bonaparte, 1836) × Siberian, Adriatic × beluga, Siberian × sterlet, and beluga × sterlet. The samples were screened for the WSIV using conventional PCR, real-time PCR, and in situ hybridization (ISH) methods. Results. In total, positive results were obtained in 231 (94.67%) out of 244 analysed samples, except for H. huso where DNA of WSIV was not identified. DNA of WSIV was most frequently detected in the kidney (97.92%), intestine (97.50%), skin (94.97%), and gills ... Article in Journal/Newspaper Acipenser baerii Beluga Beluga* Siberian sturgeon Directory of Open Access Journals: DOAJ Articles Forbes ENVELOPE(-66.550,-66.550,-67.783,-67.783) Bonaparte ENVELOPE(160.833,160.833,-83.083,-83.083) Acta Ichthyologica et Piscatoria 49 3 257 264 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
white sturgeon iridovirus WSIV aquaculture viru Aquaculture. Fisheries. Angling SH1-691 |
spellingShingle |
white sturgeon iridovirus WSIV aquaculture viru Aquaculture. Fisheries. Angling SH1-691 P. Hofsoe-Oppermann J. Kiełpińska R. Panicz S.M. Bergmann Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy |
topic_facet |
white sturgeon iridovirus WSIV aquaculture viru Aquaculture. Fisheries. Angling SH1-691 |
description |
Background. White sturgeon iridovirus (WSIV) is one of the most serious threats for sturgeon aquaculture as the mortality in juvenile individuals can reach 95%. At the turn of the century WSIV was reported among hatchery-raised white sturgeon, Acipenser transmontanus Richardson, 1836, in North America, Russian sturgeon , Acipenser gueldenstaedtii Brandt et Ratzeburg, 1833, in northern Europe but also pallid sturgeon, Scaphirhynchus albus (Forbes et Richardson, 1905) and shovelnose sturgeon, Scaphirhynchus platorynchus (Rafinesque, 1820) in North and South Dakota. The clinical signs of infection include cessation of feeding and edematous and pale gills. Those pathological changes lead to respiratory problems of the juveniles and deterioration of their overall condition. Rapid detection of pathogen particles under aquaculture conditions is crucial, therefore the principal aim of this study was to establish a molecular method for rapid and effective detection of the WSIV. This is the first study thoroughly comparing WSIV detection methods in sturgeons cultured in Poland, Germany, and Italy. Materials and methods. A total of 244 samples were collected from 82 sturgeons representing six species: beluga, Huso huso (Linnaeus, 1758); sterlet, Acipenser ruthenus Linnaeus, 1758; Siberian sturgeon, Acipenser baerii Brandt, 1869; Russian sturgeon, A. gueldenstaedtii; Atlantic sturgeon, Acipenser oxyrinchus Mitchill, 1815; and European sea sturgeon, Acipenser sturio Linnaeus, 1758, as well as five sturgeon hybrids: Siberian × Russian, Adriatic (Acipenser naccarii Bonaparte, 1836) × Siberian, Adriatic × beluga, Siberian × sterlet, and beluga × sterlet. The samples were screened for the WSIV using conventional PCR, real-time PCR, and in situ hybridization (ISH) methods. Results. In total, positive results were obtained in 231 (94.67%) out of 244 analysed samples, except for H. huso where DNA of WSIV was not identified. DNA of WSIV was most frequently detected in the kidney (97.92%), intestine (97.50%), skin (94.97%), and gills ... |
format |
Article in Journal/Newspaper |
author |
P. Hofsoe-Oppermann J. Kiełpińska R. Panicz S.M. Bergmann |
author_facet |
P. Hofsoe-Oppermann J. Kiełpińska R. Panicz S.M. Bergmann |
author_sort |
P. Hofsoe-Oppermann |
title |
Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy |
title_short |
Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy |
title_full |
Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy |
title_fullStr |
Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy |
title_full_unstemmed |
Detection of white sturgeon iridovirus (WSIV) in sturgeons (Actinopterygii: Acipenseriformes: Acipenseridae) from aquaculture facilities located in Poland, Germany, and Italy |
title_sort |
detection of white sturgeon iridovirus (wsiv) in sturgeons (actinopterygii: acipenseriformes: acipenseridae) from aquaculture facilities located in poland, germany, and italy |
publisher |
Pensoft Publishers |
publishDate |
2019 |
url |
https://doi.org/10.3750/AIEP/02568 https://doaj.org/article/3539b47a43974238a7ce6bd35fdb0448 |
long_lat |
ENVELOPE(-66.550,-66.550,-67.783,-67.783) ENVELOPE(160.833,160.833,-83.083,-83.083) |
geographic |
Forbes Bonaparte |
geographic_facet |
Forbes Bonaparte |
genre |
Acipenser baerii Beluga Beluga* Siberian sturgeon |
genre_facet |
Acipenser baerii Beluga Beluga* Siberian sturgeon |
op_source |
Acta Ichthyologica et Piscatoria, Vol 49, Iss 3, Pp 257-264 (2019) |
op_relation |
https://aiep.pensoft.net/article/26906/download/pdf/ https://aiep.pensoft.net/article/26906/download/xml/ https://aiep.pensoft.net/article/26906/ https://doaj.org/toc/1734-1515 doi:10.3750/AIEP/02568 1734-1515 https://doaj.org/article/3539b47a43974238a7ce6bd35fdb0448 |
op_doi |
https://doi.org/10.3750/AIEP/02568 |
container_title |
Acta Ichthyologica et Piscatoria |
container_volume |
49 |
container_issue |
3 |
container_start_page |
257 |
op_container_end_page |
264 |
_version_ |
1766290739052937216 |