TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum
B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percent...
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ftdoajarticles:oai:doaj.org/article:35319f5ef25a41a3b12f606f5b40940e 2023-05-15T15:18:13+02:00 TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum Haixia Wei Hongyan Xie Jiale Qu Anqi Xie Shihao Xie He Huang Jiajie Li Chao Fang Feihu Shi Huaina Qiu Yanwei Qi Xu Tian Quan Yang Jun Huang 2021-11-01T00:00:00Z https://doaj.org/article/35319f5ef25a41a3b12f606f5b40940e EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598019/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/35319f5ef25a41a3b12f606f5b40940e PLoS Neglected Tropical Diseases, Vol 15, Iss 11 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles 2022-12-31T08:01:47Z B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice. Author summary Schistosomiasis seriously jeopardizes public health and social development in tropical and subtropical regions. B cells play an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, we found the percentage and numbers of B cells increased in the infected mice (p < 0.05), and the expression of many activation and function associated molecules were also changed on B cells. B cells were served as the main cell population expressed TLR7. When TLR7 gene was knockout, the soluble egg antigen (SEA) specific antibody and activation associated molecules were decreased in S. japonicum infected mice. Additionally, SEA could induce the activation of B cells by TLR7. Finally, we found the effects of TLR7 on B cells ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Haixia Wei Hongyan Xie Jiale Qu Anqi Xie Shihao Xie He Huang Jiajie Li Chao Fang Feihu Shi Huaina Qiu Yanwei Qi Xu Tian Quan Yang Jun Huang TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, C57BL/6 mice were percutaneously infected by S. japonicum for 5–6 weeks. The percentages and numbers of B cells increased in the infected mice (p < 0.05), and many activation and function associated molecules were also changed on B cells. More splenic cells of the infected mice expressed TLR7, and B cells were served as the main cell population. Moreover, a lower level of soluble egg antigen (SEA) specific antibody and less activation associated molecules were found on the surface of splenic B cells from S. japonicum infected TLR7 gene knockout (TLR7 KO) mice compared to infected wild type (WT) mice (p < 0.05). Additionally, SEA showed a little higher ability in inducing the activation of B cells from naive WT mice than TLR7 KO mice (p < 0.05). Finally, the effects of TLR7 on B cells are dependent on the activation of NF-κB p65. Altogether, TLR7 was found modulating the splenic B cell responses in S. japonicum infected C57BL/6 mice. Author summary Schistosomiasis seriously jeopardizes public health and social development in tropical and subtropical regions. B cells play an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear. Here, we found the percentage and numbers of B cells increased in the infected mice (p < 0.05), and the expression of many activation and function associated molecules were also changed on B cells. B cells were served as the main cell population expressed TLR7. When TLR7 gene was knockout, the soluble egg antigen (SEA) specific antibody and activation associated molecules were decreased in S. japonicum infected mice. Additionally, SEA could induce the activation of B cells by TLR7. Finally, we found the effects of TLR7 on B cells ... |
format |
Article in Journal/Newspaper |
author |
Haixia Wei Hongyan Xie Jiale Qu Anqi Xie Shihao Xie He Huang Jiajie Li Chao Fang Feihu Shi Huaina Qiu Yanwei Qi Xu Tian Quan Yang Jun Huang |
author_facet |
Haixia Wei Hongyan Xie Jiale Qu Anqi Xie Shihao Xie He Huang Jiajie Li Chao Fang Feihu Shi Huaina Qiu Yanwei Qi Xu Tian Quan Yang Jun Huang |
author_sort |
Haixia Wei |
title |
TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum |
title_short |
TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum |
title_full |
TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum |
title_fullStr |
TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum |
title_full_unstemmed |
TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum |
title_sort |
tlr7 modulating b-cell immune responses in the spleen of c57bl/6 mice infected with schistosoma japonicum |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doaj.org/article/35319f5ef25a41a3b12f606f5b40940e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 11 (2021) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8598019/?tool=EBI https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 https://doaj.org/article/35319f5ef25a41a3b12f606f5b40940e |
_version_ |
1766348439830921216 |