Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions
Abstract Background Black soldier fly (Hermetia illucens) is a promising insect species to use as a novel ingredient in fish feeds. Black soldier fly larvae consists of three major fractions, namely protein, lipid, and exoskeleton. These fractions contain bioactive compounds that can modulate the gu...
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ftdoajarticles:oai:doaj.org/article:34e621d2afbf492091ed9e1814c630b2 2023-05-15T15:30:53+02:00 Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions Pabodha Weththasinghe Sérgio D. C. Rocha Ove Øyås Leidy Lagos Jon Ø. Hansen Liv T. Mydland Margareth Øverland 2022-01-01T00:00:00Z https://doi.org/10.1186/s42523-021-00161-w https://doaj.org/article/34e621d2afbf492091ed9e1814c630b2 EN eng BMC https://doi.org/10.1186/s42523-021-00161-w https://doaj.org/toc/2524-4671 doi:10.1186/s42523-021-00161-w 2524-4671 https://doaj.org/article/34e621d2afbf492091ed9e1814c630b2 Animal Microbiome, Vol 4, Iss 1, Pp 1-21 (2022) Black soldier fly Atlantic salmon Gut microbiota Predicted microbial metabolic capacity Full-fat insect meal Defatted insect meal Veterinary medicine SF600-1100 Microbiology QR1-502 article 2022 ftdoajarticles https://doi.org/10.1186/s42523-021-00161-w 2022-12-31T08:08:36Z Abstract Background Black soldier fly (Hermetia illucens) is a promising insect species to use as a novel ingredient in fish feeds. Black soldier fly larvae consists of three major fractions, namely protein, lipid, and exoskeleton. These fractions contain bioactive compounds that can modulate the gut microbiota in fish such as antimicrobial peptides, lauric acid, and chitin. However, it is not certain how, or which fractions of black solider fly would affect gut microbiota in fish. In the present study, black soldier fly larvae were processed into three different meals (full-fat, defatted and de-chitinized) and two fractions (oil and exoskeleton), and included in diets for Atlantic salmon (Salmo salar). Atlantic salmon pre-smolts were fed with these diets in comparison with a commercial-like control diet for eight weeks to investigate the effects of insect meals and fractions on the composition and predicted metabolic capacity of gut microbiota. The gut microbiota was profiled by 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models. Results The inclusion of insect meals and fractions decreased abundance of Proteobacteria and increased abundance of Firmicutes in salmon gut. The diets that contained insect chitin, i.e., insect meals or exoskeleton diets, increased abundance of chitinolytic bacteria including lactic acid bacteria and Actinomyces in salmon gut, with fish fed full-fat meal diet showing the highest abundances. The diets that contained insect lipids, i.e., insect meals and oil diets enriched Bacillaceae in fish gut. The fish fed diets containing full-fat insect meal had a unique gut microbiota composition dominated by beneficial lactic acid bacteria and Actinomyces, and showed a predicted increase in mucin degradation compared to the other diets. Conclusions The present results showed that the dietary inclusion of insect meals and fractions can differently modulate the composition and predicted metabolic capacity of gut ... Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Animal Microbiome 4 1 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Black soldier fly Atlantic salmon Gut microbiota Predicted microbial metabolic capacity Full-fat insect meal Defatted insect meal Veterinary medicine SF600-1100 Microbiology QR1-502 |
spellingShingle |
Black soldier fly Atlantic salmon Gut microbiota Predicted microbial metabolic capacity Full-fat insect meal Defatted insect meal Veterinary medicine SF600-1100 Microbiology QR1-502 Pabodha Weththasinghe Sérgio D. C. Rocha Ove Øyås Leidy Lagos Jon Ø. Hansen Liv T. Mydland Margareth Øverland Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions |
topic_facet |
Black soldier fly Atlantic salmon Gut microbiota Predicted microbial metabolic capacity Full-fat insect meal Defatted insect meal Veterinary medicine SF600-1100 Microbiology QR1-502 |
description |
Abstract Background Black soldier fly (Hermetia illucens) is a promising insect species to use as a novel ingredient in fish feeds. Black soldier fly larvae consists of three major fractions, namely protein, lipid, and exoskeleton. These fractions contain bioactive compounds that can modulate the gut microbiota in fish such as antimicrobial peptides, lauric acid, and chitin. However, it is not certain how, or which fractions of black solider fly would affect gut microbiota in fish. In the present study, black soldier fly larvae were processed into three different meals (full-fat, defatted and de-chitinized) and two fractions (oil and exoskeleton), and included in diets for Atlantic salmon (Salmo salar). Atlantic salmon pre-smolts were fed with these diets in comparison with a commercial-like control diet for eight weeks to investigate the effects of insect meals and fractions on the composition and predicted metabolic capacity of gut microbiota. The gut microbiota was profiled by 16S rRNA gene sequencing, and the predicted metabolic capacities of gut microbiota were determined using genome-scale metabolic models. Results The inclusion of insect meals and fractions decreased abundance of Proteobacteria and increased abundance of Firmicutes in salmon gut. The diets that contained insect chitin, i.e., insect meals or exoskeleton diets, increased abundance of chitinolytic bacteria including lactic acid bacteria and Actinomyces in salmon gut, with fish fed full-fat meal diet showing the highest abundances. The diets that contained insect lipids, i.e., insect meals and oil diets enriched Bacillaceae in fish gut. The fish fed diets containing full-fat insect meal had a unique gut microbiota composition dominated by beneficial lactic acid bacteria and Actinomyces, and showed a predicted increase in mucin degradation compared to the other diets. Conclusions The present results showed that the dietary inclusion of insect meals and fractions can differently modulate the composition and predicted metabolic capacity of gut ... |
format |
Article in Journal/Newspaper |
author |
Pabodha Weththasinghe Sérgio D. C. Rocha Ove Øyås Leidy Lagos Jon Ø. Hansen Liv T. Mydland Margareth Øverland |
author_facet |
Pabodha Weththasinghe Sérgio D. C. Rocha Ove Øyås Leidy Lagos Jon Ø. Hansen Liv T. Mydland Margareth Øverland |
author_sort |
Pabodha Weththasinghe |
title |
Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions |
title_short |
Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions |
title_full |
Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions |
title_fullStr |
Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions |
title_full_unstemmed |
Modulation of Atlantic salmon (Salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (Hermetia illucens) larvae meals and fractions |
title_sort |
modulation of atlantic salmon (salmo salar) gut microbiota composition and predicted metabolic capacity by feeding diets with processed black soldier fly (hermetia illucens) larvae meals and fractions |
publisher |
BMC |
publishDate |
2022 |
url |
https://doi.org/10.1186/s42523-021-00161-w https://doaj.org/article/34e621d2afbf492091ed9e1814c630b2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Animal Microbiome, Vol 4, Iss 1, Pp 1-21 (2022) |
op_relation |
https://doi.org/10.1186/s42523-021-00161-w https://doaj.org/toc/2524-4671 doi:10.1186/s42523-021-00161-w 2524-4671 https://doaj.org/article/34e621d2afbf492091ed9e1814c630b2 |
op_doi |
https://doi.org/10.1186/s42523-021-00161-w |
container_title |
Animal Microbiome |
container_volume |
4 |
container_issue |
1 |
_version_ |
1766361353746907136 |