Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3
Objective: To examine the effects of Tribulus terrestris L. (T. terrestris) extract on the modulation of calcium channels to evaluate its use in topical agents for treatment of atopic dermatitis. Methods: The 70% methanol extract of T. terrestris was prepared. Human HEK293T cells with over-expressed...
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ftdoajarticles:oai:doaj.org/article:34db00111abd4ab584a123d2679bdd86 2023-05-15T15:07:40+02:00 Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3 Joo Hyun Nam Hyo Won Jung Young-Won Chin Woo Kyung Kim Hyo Sang Bae 2016-07-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.05.006 https://doaj.org/article/34db00111abd4ab584a123d2679bdd86 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116301617 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.05.006 https://doaj.org/article/34db00111abd4ab584a123d2679bdd86 Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 7, Pp 580-585 (2016) Tribulus terrestris L Atopic dermatitis Ion channels ORAI1 TRPV3 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.05.006 2022-12-31T14:24:42Z Objective: To examine the effects of Tribulus terrestris L. (T. terrestris) extract on the modulation of calcium channels to evaluate its use in topical agents for treatment of atopic dermatitis. Methods: The 70% methanol extract of T. terrestris was prepared. Human HEK293T cells with over-expressed calcium release-activated calcium channel protein 1 (Orai1), transient receptor potential vanilloid 1, or transient receptor potential vanilloid 3 (TRPV3) were treated with T. terrestris extract. Modulation of ion channels was measured using a conventional whole-cell patch-clamp technique. Results: T. terrestris extract (100 μg/mL) significantly inhibited Orai1 activity in Orai1-stromal interaction molecule 1 co-overexpressed HEK293T cells. In addition, T. terrestris extract significantly increased the TRPV3 activity compared with 2-Aminoethyl diphenylborinate (100 μmol/L), which induces the full activation of TRPV3. Conclusions: Our results suggest that T. terrestris extract may have a therapeutic potential for recovery of abnormal skin barrier pathologies in atopic dermatitis through modulating the activities of calcium ion channels, Orai1 and TRPV3. This is the first study to report the modulatory effect of a medicinal plant on the function of ion channels in skin barrier. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 7 580 585 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Tribulus terrestris L Atopic dermatitis Ion channels ORAI1 TRPV3 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Tribulus terrestris L Atopic dermatitis Ion channels ORAI1 TRPV3 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Joo Hyun Nam Hyo Won Jung Young-Won Chin Woo Kyung Kim Hyo Sang Bae Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3 |
topic_facet |
Tribulus terrestris L Atopic dermatitis Ion channels ORAI1 TRPV3 Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To examine the effects of Tribulus terrestris L. (T. terrestris) extract on the modulation of calcium channels to evaluate its use in topical agents for treatment of atopic dermatitis. Methods: The 70% methanol extract of T. terrestris was prepared. Human HEK293T cells with over-expressed calcium release-activated calcium channel protein 1 (Orai1), transient receptor potential vanilloid 1, or transient receptor potential vanilloid 3 (TRPV3) were treated with T. terrestris extract. Modulation of ion channels was measured using a conventional whole-cell patch-clamp technique. Results: T. terrestris extract (100 μg/mL) significantly inhibited Orai1 activity in Orai1-stromal interaction molecule 1 co-overexpressed HEK293T cells. In addition, T. terrestris extract significantly increased the TRPV3 activity compared with 2-Aminoethyl diphenylborinate (100 μmol/L), which induces the full activation of TRPV3. Conclusions: Our results suggest that T. terrestris extract may have a therapeutic potential for recovery of abnormal skin barrier pathologies in atopic dermatitis through modulating the activities of calcium ion channels, Orai1 and TRPV3. This is the first study to report the modulatory effect of a medicinal plant on the function of ion channels in skin barrier. |
format |
Article in Journal/Newspaper |
author |
Joo Hyun Nam Hyo Won Jung Young-Won Chin Woo Kyung Kim Hyo Sang Bae |
author_facet |
Joo Hyun Nam Hyo Won Jung Young-Won Chin Woo Kyung Kim Hyo Sang Bae |
author_sort |
Joo Hyun Nam |
title |
Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3 |
title_short |
Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3 |
title_full |
Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3 |
title_fullStr |
Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3 |
title_full_unstemmed |
Modulatory effects of the fruits of Tribulus terrestris L. on the function of atopic dermatitis-related calcium channels, Orai1 and TRPV3 |
title_sort |
modulatory effects of the fruits of tribulus terrestris l. on the function of atopic dermatitis-related calcium channels, orai1 and trpv3 |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2016.05.006 https://doaj.org/article/34db00111abd4ab584a123d2679bdd86 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 7, Pp 580-585 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116301617 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.05.006 https://doaj.org/article/34db00111abd4ab584a123d2679bdd86 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.05.006 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
7 |
container_start_page |
580 |
op_container_end_page |
585 |
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1766339122365988864 |