Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse.
Splenomegaly, an enlargement of the spleen, is a known clinical sign of the parasitic disease, human African trypanosomiasis. This study follows the development of splenomegaly in a group of mice over multiple infection points, using a non-invasive imaging modality, magnetic resonance imaging (MRI)....
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ftdoajarticles:oai:doaj.org/article:34cc5d90767a4278908f22a9e0ea96b7 2023-05-15T15:06:02+02:00 Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. Samantha Paterson William Matthew Holmes Jean Rodgers 2022-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010962 https://doaj.org/article/34cc5d90767a4278908f22a9e0ea96b7 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010962 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010962 https://doaj.org/article/34cc5d90767a4278908f22a9e0ea96b7 PLoS Neglected Tropical Diseases, Vol 16, Iss 12, p e0010962 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010962 2022-12-30T19:28:45Z Splenomegaly, an enlargement of the spleen, is a known clinical sign of the parasitic disease, human African trypanosomiasis. This study follows the development of splenomegaly in a group of mice over multiple infection points, using a non-invasive imaging modality, magnetic resonance imaging (MRI). CD-1 mice infected with GVR35 T.b. brucei demonstrated a significant increase in spleen size from day 7 post-infection, with changes in the spleen tracked in individual animals over five time points. At the final time point, the mean spleen weight calculated using the spleen volume from the MR images was compared with the post-mortem gross spleen weight. No significant difference was detected between the two methods (1.62 ± 0.06g using MRI and 1.51 ± 0.04g gross weight, p = 0.554). Haematology and histological analysis were also performed, giving additional insight into splenomegaly for the GVR35 strain of infection. The study demonstrates that MRI is a useful tool when examining changes in organ volume throughout HAT infection and may be applicable in the investigation of a range of conditions where changes in organ volume occur and MRI has not been used previously. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 16 12 e0010962 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Samantha Paterson William Matthew Holmes Jean Rodgers Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Splenomegaly, an enlargement of the spleen, is a known clinical sign of the parasitic disease, human African trypanosomiasis. This study follows the development of splenomegaly in a group of mice over multiple infection points, using a non-invasive imaging modality, magnetic resonance imaging (MRI). CD-1 mice infected with GVR35 T.b. brucei demonstrated a significant increase in spleen size from day 7 post-infection, with changes in the spleen tracked in individual animals over five time points. At the final time point, the mean spleen weight calculated using the spleen volume from the MR images was compared with the post-mortem gross spleen weight. No significant difference was detected between the two methods (1.62 ± 0.06g using MRI and 1.51 ± 0.04g gross weight, p = 0.554). Haematology and histological analysis were also performed, giving additional insight into splenomegaly for the GVR35 strain of infection. The study demonstrates that MRI is a useful tool when examining changes in organ volume throughout HAT infection and may be applicable in the investigation of a range of conditions where changes in organ volume occur and MRI has not been used previously. |
format |
Article in Journal/Newspaper |
author |
Samantha Paterson William Matthew Holmes Jean Rodgers |
author_facet |
Samantha Paterson William Matthew Holmes Jean Rodgers |
author_sort |
Samantha Paterson |
title |
Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. |
title_short |
Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. |
title_full |
Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. |
title_fullStr |
Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. |
title_full_unstemmed |
Serial magnetic resonance imaging of splenomegaly in the Trypanosoma brucei infected mouse. |
title_sort |
serial magnetic resonance imaging of splenomegaly in the trypanosoma brucei infected mouse. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010962 https://doaj.org/article/34cc5d90767a4278908f22a9e0ea96b7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 12, p e0010962 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010962 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010962 https://doaj.org/article/34cc5d90767a4278908f22a9e0ea96b7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0010962 |
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PLOS Neglected Tropical Diseases |
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16 |
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12 |
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e0010962 |
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