Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice.
Alveolar echinococcosis (AE) in humans is a parasitic disease characterized by severe damage to the liver and occasionally other organs. AE is caused by infection with the metacestode (larval) stage of the fox tapeworm Echinococcus multilocularis, usually infecting small rodents as natural intermedi...
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ftdoajarticles:oai:doaj.org/article:34bf990cdd4a49db91146a69fa242684 2023-05-15T15:08:56+02:00 Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice. Tatiana Küster Corina Hermann Andrew Hemphill Bruno Gottstein Markus Spiliotis 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002235 https://doaj.org/article/34bf990cdd4a49db91146a69fa242684 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3662659?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0002235 https://doaj.org/article/34bf990cdd4a49db91146a69fa242684 PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2235 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002235 2022-12-31T14:05:09Z Alveolar echinococcosis (AE) in humans is a parasitic disease characterized by severe damage to the liver and occasionally other organs. AE is caused by infection with the metacestode (larval) stage of the fox tapeworm Echinococcus multilocularis, usually infecting small rodents as natural intermediate hosts. Conventionally, human AE is chemotherapeutically treated with mebendazole or albendazole. There is, however still the need for improved chemotherapeutical options. Primary in vivo studies on drugs of interest are commonly performed in small laboratory animals such as mice and Mongolian jirds, and in most cases, a secondary infection model is used, whereby E. multilocularis metacestodes are directly injected into the peritoneal cavity or into the liver. Disadvantages of this methodological approach include risk of injury to organs during the inoculation and, most notably, a limitation in the macroscopic (visible) assessment of treatment efficacy. Thus, in order to monitor the efficacy of chemotherapeutical treatment, animals have to be euthanized and the parasite tissue dissected. In the present study, mice were infected with E. multilocularis metacestodes through the subcutaneous route and were then subjected to chemotherapy employing albendazole. Serological responses to infection were comparatively assessed in mice infected by the conventional intraperitoneal route. We demonstrate that the subcutaneous infection model for secondary AE facilitates the assessment of the progress of infection and drug treatment in the live animal. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 5 e2235 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Tatiana Küster Corina Hermann Andrew Hemphill Bruno Gottstein Markus Spiliotis Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Alveolar echinococcosis (AE) in humans is a parasitic disease characterized by severe damage to the liver and occasionally other organs. AE is caused by infection with the metacestode (larval) stage of the fox tapeworm Echinococcus multilocularis, usually infecting small rodents as natural intermediate hosts. Conventionally, human AE is chemotherapeutically treated with mebendazole or albendazole. There is, however still the need for improved chemotherapeutical options. Primary in vivo studies on drugs of interest are commonly performed in small laboratory animals such as mice and Mongolian jirds, and in most cases, a secondary infection model is used, whereby E. multilocularis metacestodes are directly injected into the peritoneal cavity or into the liver. Disadvantages of this methodological approach include risk of injury to organs during the inoculation and, most notably, a limitation in the macroscopic (visible) assessment of treatment efficacy. Thus, in order to monitor the efficacy of chemotherapeutical treatment, animals have to be euthanized and the parasite tissue dissected. In the present study, mice were infected with E. multilocularis metacestodes through the subcutaneous route and were then subjected to chemotherapy employing albendazole. Serological responses to infection were comparatively assessed in mice infected by the conventional intraperitoneal route. We demonstrate that the subcutaneous infection model for secondary AE facilitates the assessment of the progress of infection and drug treatment in the live animal. |
format |
Article in Journal/Newspaper |
author |
Tatiana Küster Corina Hermann Andrew Hemphill Bruno Gottstein Markus Spiliotis |
author_facet |
Tatiana Küster Corina Hermann Andrew Hemphill Bruno Gottstein Markus Spiliotis |
author_sort |
Tatiana Küster |
title |
Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice. |
title_short |
Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice. |
title_full |
Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice. |
title_fullStr |
Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice. |
title_full_unstemmed |
Subcutaneous infection model facilitates treatment assessment of secondary Alveolar echinococcosis in mice. |
title_sort |
subcutaneous infection model facilitates treatment assessment of secondary alveolar echinococcosis in mice. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002235 https://doaj.org/article/34bf990cdd4a49db91146a69fa242684 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2235 (2013) |
op_relation |
http://europepmc.org/articles/PMC3662659?pdf=render https://doaj.org/toc/1935-2735 1935-2735 doi:10.1371/journal.pntd.0002235 https://doaj.org/article/34bf990cdd4a49db91146a69fa242684 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002235 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
container_issue |
5 |
container_start_page |
e2235 |
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1766340199076331520 |