Ameliorative Effects of Peptides Derived from Oyster ( Crassostrea gigas ) on Immunomodulatory Function and Gut Microbiota Structure in Cyclophosphamide-Treated Mice

• Published in: Marine Drugs
• Main Authors: Xing-Wei Xiang, Hui-Zhen Zheng, Rui Wang, Hui Chen, Jin-Xing Xiao, Bin Zheng, Shu-Lai Liu, Yu-Ting Ding
• Format: Article in Journal/Newspaper
• Language: English
• Published: MDPI AG 2021
• Subjects: oyster ( Crassostrea gigas ); peptides; cyclophosphamide; immunomodulatory; gut microbiota; short-chain fatty acid; Biology (General); QH301-705.5; Crassostrea gigas
• Online Access: https://doi.org/10.3390/md19080456; https://doaj.org/article/348c21a1e4e2409498114f1d952a2364

The intestinal flora is recognized as a significant contributor to the immune system. In this research, the protective effects of oyster peptides on immune regulation and intestinal microbiota were investigated in mice treated with cyclophosphamide. The results showed that oyster peptides restored the indexes of thymus, spleen and liver, stimulated cytokines secretion and promoted the relative mRNA levels of Th1/Th2 cytokines (IL-2, IFN-γ, IL-4 and IL-10). The mRNA levels of Occludin, Claudin-1, ZO-1, and Mucin-2 were up-regulated, and the NF-κB signaling pathway was also activated after oyster peptides administration. Furthermore, oyster peptides treatment reduced the proportion of Firmicutes / Bacteroidetes , increased the relative abundance of Alistipes , Lactobacillus , Rikenell and the content of short-chain fatty acids, and reversed the composition of intestinal microflora similar to that of normal mice. In conclusion, oyster peptides effectively ameliorated cyclophosphamide-induced intestinal damage and modified gut microbiota structure in mice, and might be utilized as a beneficial ingredient in functional foods for immune regulation.