Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity.
Plasmodium vivax is the most widespread cause of human malaria. Recent reports of drug resistant vivax malaria and the challenge of eradicating the dormant liver forms increase the importance of vaccine development against this relapsing disease. P. vivax reticulocyte binding protein 1a (PvRBP1a) is...
Published in: | PLOS Neglected Tropical Diseases |
---|---|
Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2022
|
Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pntd.0010492 https://doaj.org/article/3436a76552fa4fde89759ad3b0038c8a |
id |
ftdoajarticles:oai:doaj.org/article:3436a76552fa4fde89759ad3b0038c8a |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3436a76552fa4fde89759ad3b0038c8a 2023-05-15T15:11:45+02:00 Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. Ji-Hoon Park Min-Hee Kim Edwin Sutanto Seok-Won Na Min-Jae Kim Joon Sup Yeom Myat Htut Nyunt Mohammed Mohieldien Abbas Elfaki Muzamil Mahdi Abdel Hamid Seok Ho Cha Sisay Getachew Alemu Kanlaya Sriprawat Nicholas M Anstey Matthew J Grigg Bridget E Barber Timothy William Qi Gao Yaobao Liu Richard D Pearson Ric N Price Francois Nosten Sung-Il Yoon Joo Hwan No Eun-Taek Han Sarah Auburn Bruce Russell Jin-Hee Han 2022-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0010492 https://doaj.org/article/3436a76552fa4fde89759ad3b0038c8a EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0010492 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010492 https://doaj.org/article/3436a76552fa4fde89759ad3b0038c8a PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010492 (2022) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2022 ftdoajarticles https://doi.org/10.1371/journal.pntd.0010492 2023-01-15T01:24:57Z Plasmodium vivax is the most widespread cause of human malaria. Recent reports of drug resistant vivax malaria and the challenge of eradicating the dormant liver forms increase the importance of vaccine development against this relapsing disease. P. vivax reticulocyte binding protein 1a (PvRBP1a) is a potential vaccine candidate, which is involved in red cell tropism, a crucial step in the merozoite invasion of host reticulocytes. As part of the initial evaluation of the PvRBP1a vaccine candidate, we investigated its genetic diversity and antigenicity using geographically diverse clinical isolates. We analysed pvrbp1a genetic polymorphisms using 202 vivax clinical isolates from six countries. Pvrbp1a was separated into six regions based on specific domain features, sequence conserved/polymorphic regions, and the reticulocyte binding like (RBL) domains. In the fragmented gene sequence analysis, PvRBP1a region II (RII) and RIII (head and tail structure homolog, 152-625 aa.) showed extensive polymorphism caused by random point mutations. The haplotype network of these polymorphic regions was classified into three clusters that converged to independent populations. Antigenicity screening was performed using recombinant proteins PvRBP1a-N (157-560 aa.) and PvRBP1a-C (606-962 aa.), which contained head and tail structure region and sequence conserved region, respectively. Sensitivity against PvRBP1a-N (46.7%) was higher than PvRBP1a-C (17.8%). PvRBP1a-N was reported as a reticulocyte binding domain and this study identified a linear epitope with moderate antigenicity, thus an attractive domain for merozoite invasion-blocking vaccine development. However, our study highlights that a global PvRBP1a-based vaccine design needs to overcome several difficulties due to three distinct genotypes and low antigenicity levels. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Random Point ENVELOPE(-132.245,-132.245,53.209,53.209) PLOS Neglected Tropical Diseases 16 6 e0010492 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ji-Hoon Park Min-Hee Kim Edwin Sutanto Seok-Won Na Min-Jae Kim Joon Sup Yeom Myat Htut Nyunt Mohammed Mohieldien Abbas Elfaki Muzamil Mahdi Abdel Hamid Seok Ho Cha Sisay Getachew Alemu Kanlaya Sriprawat Nicholas M Anstey Matthew J Grigg Bridget E Barber Timothy William Qi Gao Yaobao Liu Richard D Pearson Ric N Price Francois Nosten Sung-Il Yoon Joo Hwan No Eun-Taek Han Sarah Auburn Bruce Russell Jin-Hee Han Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Plasmodium vivax is the most widespread cause of human malaria. Recent reports of drug resistant vivax malaria and the challenge of eradicating the dormant liver forms increase the importance of vaccine development against this relapsing disease. P. vivax reticulocyte binding protein 1a (PvRBP1a) is a potential vaccine candidate, which is involved in red cell tropism, a crucial step in the merozoite invasion of host reticulocytes. As part of the initial evaluation of the PvRBP1a vaccine candidate, we investigated its genetic diversity and antigenicity using geographically diverse clinical isolates. We analysed pvrbp1a genetic polymorphisms using 202 vivax clinical isolates from six countries. Pvrbp1a was separated into six regions based on specific domain features, sequence conserved/polymorphic regions, and the reticulocyte binding like (RBL) domains. In the fragmented gene sequence analysis, PvRBP1a region II (RII) and RIII (head and tail structure homolog, 152-625 aa.) showed extensive polymorphism caused by random point mutations. The haplotype network of these polymorphic regions was classified into three clusters that converged to independent populations. Antigenicity screening was performed using recombinant proteins PvRBP1a-N (157-560 aa.) and PvRBP1a-C (606-962 aa.), which contained head and tail structure region and sequence conserved region, respectively. Sensitivity against PvRBP1a-N (46.7%) was higher than PvRBP1a-C (17.8%). PvRBP1a-N was reported as a reticulocyte binding domain and this study identified a linear epitope with moderate antigenicity, thus an attractive domain for merozoite invasion-blocking vaccine development. However, our study highlights that a global PvRBP1a-based vaccine design needs to overcome several difficulties due to three distinct genotypes and low antigenicity levels. |
format |
Article in Journal/Newspaper |
author |
Ji-Hoon Park Min-Hee Kim Edwin Sutanto Seok-Won Na Min-Jae Kim Joon Sup Yeom Myat Htut Nyunt Mohammed Mohieldien Abbas Elfaki Muzamil Mahdi Abdel Hamid Seok Ho Cha Sisay Getachew Alemu Kanlaya Sriprawat Nicholas M Anstey Matthew J Grigg Bridget E Barber Timothy William Qi Gao Yaobao Liu Richard D Pearson Ric N Price Francois Nosten Sung-Il Yoon Joo Hwan No Eun-Taek Han Sarah Auburn Bruce Russell Jin-Hee Han |
author_facet |
Ji-Hoon Park Min-Hee Kim Edwin Sutanto Seok-Won Na Min-Jae Kim Joon Sup Yeom Myat Htut Nyunt Mohammed Mohieldien Abbas Elfaki Muzamil Mahdi Abdel Hamid Seok Ho Cha Sisay Getachew Alemu Kanlaya Sriprawat Nicholas M Anstey Matthew J Grigg Bridget E Barber Timothy William Qi Gao Yaobao Liu Richard D Pearson Ric N Price Francois Nosten Sung-Il Yoon Joo Hwan No Eun-Taek Han Sarah Auburn Bruce Russell Jin-Hee Han |
author_sort |
Ji-Hoon Park |
title |
Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. |
title_short |
Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. |
title_full |
Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. |
title_fullStr |
Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. |
title_full_unstemmed |
Geographical distribution and genetic diversity of Plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. |
title_sort |
geographical distribution and genetic diversity of plasmodium vivax reticulocyte binding protein 1a correlates with patient antigenicity. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2022 |
url |
https://doi.org/10.1371/journal.pntd.0010492 https://doaj.org/article/3436a76552fa4fde89759ad3b0038c8a |
long_lat |
ENVELOPE(-132.245,-132.245,53.209,53.209) |
geographic |
Arctic Random Point |
geographic_facet |
Arctic Random Point |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 16, Iss 6, p e0010492 (2022) |
op_relation |
https://doi.org/10.1371/journal.pntd.0010492 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0010492 https://doaj.org/article/3436a76552fa4fde89759ad3b0038c8a |
op_doi |
https://doi.org/10.1371/journal.pntd.0010492 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
16 |
container_issue |
6 |
container_start_page |
e0010492 |
_version_ |
1766342554603749376 |