Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2)
Abstract Background The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asym...
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ftdoajarticles:oai:doaj.org/article:3412d4fa62344a9cb5f31a67388992b0 2023-05-15T15:15:10+02:00 Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2) Monica Ararat-Sarria Cesar Camilo Prado Milena Camargo Laura Tatiana Ospina Paola Andrea Camargo Hernando Curtidor Manuel Alfonso Patarroyo 2020-02-01T00:00:00Z https://doi.org/10.1186/s12936-020-3142-y https://doaj.org/article/3412d4fa62344a9cb5f31a67388992b0 EN eng BMC https://doi.org/10.1186/s12936-020-3142-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-3142-y 1475-2875 https://doaj.org/article/3412d4fa62344a9cb5f31a67388992b0 Malaria Journal, Vol 19, Iss 1, Pp 1-13 (2020) Malaria Plasmodium falciparum FCB2 Sexual differentiation Mosquito infectivity Gametocyte Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-3142-y 2022-12-31T05:44:30Z Abstract Background The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asymptomatic patients associated with host-vector transmission. This highlights the importance of studying the parasite’s sexual forms (gametocytes) which are involved in this phase of the parasite’s life-cycle. Some African and Asian strains of Plasmodium falciparum have been fully characterized regarding sexual forms’ production; however, few Latin-American strains have been so characterized. This study was aimed at characterizing the Colombian FCB2 strain as a gametocyte producer able to infect mosquitoes. Methods Gametocyte production was induced in in vitro cultured P. falciparum FCB2 and 3D7 strains. Pfap2g and Pfs25 gene expression was detected in FCB2 strain gametocyte culture by RT-PCR. Comparative analysis of gametocytes obtained from both strains was made (counts and morphological changes). In vitro zygote formation from FCB2 gametocytes was induced by incubating a gametocyte culture sample at 27 °C for 20 min. A controlled Anopheles albimanus infection was made using an artificial feed system with cultured FCB2 gametocytes (14–15 days old). Mosquito midgut dissection was then carried out for analyzing oocysts. Results The FCB2 strain expressed Pfap2g, Pfs16, Pfg27/25 and Pfs25 sexual differentiation-related genes after in vitro sexual differentiation induction, producing gametocytes that conserved the expected morphological features. The amount of FCB2 gametocytes produced was similar to that from the 3D7 strain. FCB2 gametocytes were differentiated into zygotes and ookinetes after an in vitro low-temperature stimulus and infected An. albimanus mosquitoes, developing to oocyst stage. Conclusions Even with the history of long-term FCB2 strain in vitro culture maintenance, it has retained its sexual ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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English |
topic |
Malaria Plasmodium falciparum FCB2 Sexual differentiation Mosquito infectivity Gametocyte Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Plasmodium falciparum FCB2 Sexual differentiation Mosquito infectivity Gametocyte Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Monica Ararat-Sarria Cesar Camilo Prado Milena Camargo Laura Tatiana Ospina Paola Andrea Camargo Hernando Curtidor Manuel Alfonso Patarroyo Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2) |
topic_facet |
Malaria Plasmodium falciparum FCB2 Sexual differentiation Mosquito infectivity Gametocyte Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The epidemiological control of malaria has been hampered by the appearance of parasite resistance to anti-malarial drugs and by the resistance of mosquito vectors to control measures. This has also been associated with weak transmission control, mostly due to poor control of asymptomatic patients associated with host-vector transmission. This highlights the importance of studying the parasite’s sexual forms (gametocytes) which are involved in this phase of the parasite’s life-cycle. Some African and Asian strains of Plasmodium falciparum have been fully characterized regarding sexual forms’ production; however, few Latin-American strains have been so characterized. This study was aimed at characterizing the Colombian FCB2 strain as a gametocyte producer able to infect mosquitoes. Methods Gametocyte production was induced in in vitro cultured P. falciparum FCB2 and 3D7 strains. Pfap2g and Pfs25 gene expression was detected in FCB2 strain gametocyte culture by RT-PCR. Comparative analysis of gametocytes obtained from both strains was made (counts and morphological changes). In vitro zygote formation from FCB2 gametocytes was induced by incubating a gametocyte culture sample at 27 °C for 20 min. A controlled Anopheles albimanus infection was made using an artificial feed system with cultured FCB2 gametocytes (14–15 days old). Mosquito midgut dissection was then carried out for analyzing oocysts. Results The FCB2 strain expressed Pfap2g, Pfs16, Pfg27/25 and Pfs25 sexual differentiation-related genes after in vitro sexual differentiation induction, producing gametocytes that conserved the expected morphological features. The amount of FCB2 gametocytes produced was similar to that from the 3D7 strain. FCB2 gametocytes were differentiated into zygotes and ookinetes after an in vitro low-temperature stimulus and infected An. albimanus mosquitoes, developing to oocyst stage. Conclusions Even with the history of long-term FCB2 strain in vitro culture maintenance, it has retained its sexual ... |
format |
Article in Journal/Newspaper |
author |
Monica Ararat-Sarria Cesar Camilo Prado Milena Camargo Laura Tatiana Ospina Paola Andrea Camargo Hernando Curtidor Manuel Alfonso Patarroyo |
author_facet |
Monica Ararat-Sarria Cesar Camilo Prado Milena Camargo Laura Tatiana Ospina Paola Andrea Camargo Hernando Curtidor Manuel Alfonso Patarroyo |
author_sort |
Monica Ararat-Sarria |
title |
Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2) |
title_short |
Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2) |
title_full |
Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2) |
title_fullStr |
Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2) |
title_full_unstemmed |
Sexual forms obtained in a continuous in vitro cultured Colombian strain of Plasmodium falciparum (FCB2) |
title_sort |
sexual forms obtained in a continuous in vitro cultured colombian strain of plasmodium falciparum (fcb2) |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-3142-y https://doaj.org/article/3412d4fa62344a9cb5f31a67388992b0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-13 (2020) |
op_relation |
https://doi.org/10.1186/s12936-020-3142-y https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-3142-y 1475-2875 https://doaj.org/article/3412d4fa62344a9cb5f31a67388992b0 |
op_doi |
https://doi.org/10.1186/s12936-020-3142-y |
container_title |
Malaria Journal |
container_volume |
19 |
container_issue |
1 |
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1766345549641940992 |