Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis.
Extracellular RNAs (ex-RNAs) are secreted by cells through different means that may involve association with proteins, lipoproteins or extracellular vesicles (EV). In the context of parasitism, ex-RNAs represent new and exciting communication intermediaries with promising potential as novel biomarke...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:34128e3bda1d4ebe9629a441bd91695b 2023-05-15T15:15:01+02:00 Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis. María Eugenia Ancarola Gabriel Lichtenstein Johannes Herbig Nancy Holroyd Mara Mariconti Enrico Brunetti Matthew Berriman Krystyna Albrecht Antonio Marcilla Mara Cecilia Rosenzvit Laura Kamenetzky Klaus Brehm Marcela Cucher 2020-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008890 https://doaj.org/article/34128e3bda1d4ebe9629a441bd91695b EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008890 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008890 https://doaj.org/article/34128e3bda1d4ebe9629a441bd91695b PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008890 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008890 2022-12-31T07:50:09Z Extracellular RNAs (ex-RNAs) are secreted by cells through different means that may involve association with proteins, lipoproteins or extracellular vesicles (EV). In the context of parasitism, ex-RNAs represent new and exciting communication intermediaries with promising potential as novel biomarkers. In the last years, it was shown that helminth parasites secrete ex-RNAs, however, most work mainly focused on RNA secretion mediated by EV. Ex-RNA study is of special interest in those helminth infections that still lack biomarkers for early and/or follow-up diagnosis, such as echinococcosis, a neglected zoonotic disease caused by cestodes of the genus Echinococcus. In this work, we have characterised the ex-RNA profile secreted by in vitro grown metacestodes of Echinococcus multilocularis, the casuative agent of alveolar echinococcosis. We have used high throughput RNA-sequencing together with RT-qPCR to characterise the ex-RNA profile secreted towards the extra- and intra-parasite milieus in EV-enriched and EV-depleted fractions. We show that a polarized secretion of small RNAs takes place, with microRNAs mainly secreted to the extra-parasite milieu and rRNA- and tRNA-derived sequences mostly secreted to the intra-parasite milieu. In addition, we show by nanoparticle tracking analyses that viable metacestodes secrete EV mainly into the metacestode inner vesicular fluid (MVF); however, the number of nanoparticles in culture medium and MVF increases > 10-fold when metacestodes show signs of tegument impairment. Interestingly, we confirm the presence of host miRNAs in the intra-parasite milieu, implying their internalization and transport through the tegument towards the MVF. Finally, our assessment of the detection of Echinococcus miRNAs in patient samples by RT-qPCR yielded negative results suggesting the tested miRNAs may not be good biomarkers for this disease. A comprehensive study of the secretion mechanisms throughout the life cycle of these parasites will help to understand parasite interaction with the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 11 e0008890 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 María Eugenia Ancarola Gabriel Lichtenstein Johannes Herbig Nancy Holroyd Mara Mariconti Enrico Brunetti Matthew Berriman Krystyna Albrecht Antonio Marcilla Mara Cecilia Rosenzvit Laura Kamenetzky Klaus Brehm Marcela Cucher Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Extracellular RNAs (ex-RNAs) are secreted by cells through different means that may involve association with proteins, lipoproteins or extracellular vesicles (EV). In the context of parasitism, ex-RNAs represent new and exciting communication intermediaries with promising potential as novel biomarkers. In the last years, it was shown that helminth parasites secrete ex-RNAs, however, most work mainly focused on RNA secretion mediated by EV. Ex-RNA study is of special interest in those helminth infections that still lack biomarkers for early and/or follow-up diagnosis, such as echinococcosis, a neglected zoonotic disease caused by cestodes of the genus Echinococcus. In this work, we have characterised the ex-RNA profile secreted by in vitro grown metacestodes of Echinococcus multilocularis, the casuative agent of alveolar echinococcosis. We have used high throughput RNA-sequencing together with RT-qPCR to characterise the ex-RNA profile secreted towards the extra- and intra-parasite milieus in EV-enriched and EV-depleted fractions. We show that a polarized secretion of small RNAs takes place, with microRNAs mainly secreted to the extra-parasite milieu and rRNA- and tRNA-derived sequences mostly secreted to the intra-parasite milieu. In addition, we show by nanoparticle tracking analyses that viable metacestodes secrete EV mainly into the metacestode inner vesicular fluid (MVF); however, the number of nanoparticles in culture medium and MVF increases > 10-fold when metacestodes show signs of tegument impairment. Interestingly, we confirm the presence of host miRNAs in the intra-parasite milieu, implying their internalization and transport through the tegument towards the MVF. Finally, our assessment of the detection of Echinococcus miRNAs in patient samples by RT-qPCR yielded negative results suggesting the tested miRNAs may not be good biomarkers for this disease. A comprehensive study of the secretion mechanisms throughout the life cycle of these parasites will help to understand parasite interaction with the ... |
format |
Article in Journal/Newspaper |
author |
María Eugenia Ancarola Gabriel Lichtenstein Johannes Herbig Nancy Holroyd Mara Mariconti Enrico Brunetti Matthew Berriman Krystyna Albrecht Antonio Marcilla Mara Cecilia Rosenzvit Laura Kamenetzky Klaus Brehm Marcela Cucher |
author_facet |
María Eugenia Ancarola Gabriel Lichtenstein Johannes Herbig Nancy Holroyd Mara Mariconti Enrico Brunetti Matthew Berriman Krystyna Albrecht Antonio Marcilla Mara Cecilia Rosenzvit Laura Kamenetzky Klaus Brehm Marcela Cucher |
author_sort |
María Eugenia Ancarola |
title |
Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis. |
title_short |
Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis. |
title_full |
Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis. |
title_fullStr |
Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis. |
title_full_unstemmed |
Extracellular non-coding RNA signatures of the metacestode stage of Echinococcus multilocularis. |
title_sort |
extracellular non-coding rna signatures of the metacestode stage of echinococcus multilocularis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008890 https://doaj.org/article/34128e3bda1d4ebe9629a441bd91695b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 11, p e0008890 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008890 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008890 https://doaj.org/article/34128e3bda1d4ebe9629a441bd91695b |
op_doi |
https://doi.org/10.1371/journal.pntd.0008890 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
11 |
container_start_page |
e0008890 |
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1766345408831815680 |