Species Identification of Caviar Based on Multiple DNA Barcoding
This study aimed to explore the applicability of DNA barcoding for assessing the authenticity of caviar on the Chinese market. A set of universal COI primers and two sets of designed primers based on COI and D-loop genes were used to identify maternal species of samples from 21 batches of caviar. Th...
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MDPI AG
2023
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Online Access: | https://doi.org/10.3390/molecules28135046 https://doaj.org/article/3401d9cf31ce4b92897922ff016cf1c9 |
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ftdoajarticles:oai:doaj.org/article:3401d9cf31ce4b92897922ff016cf1c9 2023-07-30T03:55:24+02:00 Species Identification of Caviar Based on Multiple DNA Barcoding Qingqing Hu Yingqiu Pan Huili Xia Kexin Yu Yian Yao Feng Guan 2023-06-01T00:00:00Z https://doi.org/10.3390/molecules28135046 https://doaj.org/article/3401d9cf31ce4b92897922ff016cf1c9 EN eng MDPI AG https://www.mdpi.com/1420-3049/28/13/5046 https://doaj.org/toc/1420-3049 doi:10.3390/molecules28135046 1420-3049 https://doaj.org/article/3401d9cf31ce4b92897922ff016cf1c9 Molecules, Vol 28, Iss 5046, p 5046 (2023) DNA barcoding caviar COI gene D-loop gene species identification authenticity identification Organic chemistry QD241-441 article 2023 ftdoajarticles https://doi.org/10.3390/molecules28135046 2023-07-16T00:34:55Z This study aimed to explore the applicability of DNA barcoding for assessing the authenticity of caviar on the Chinese market. A set of universal COI primers and two sets of designed primers based on COI and D-loop genes were used to identify maternal species of samples from 21 batches of caviar. The results showed that the PCR products from three sets of primers had more than 98% similarity to the sequences in database. The COI gene could not distinguish sturgeons with closed genetic relationships, while D-loop gene could effectively improve the accuracy of DNA barcoding and was more suitable to the identification of interspecific sturgeon than the COI gene. The neighbor-joining dendrogram further confirmed the applicability and accuracy of COI and D-loop genes in identifying maternal relatives of caviar ( Acipenser baerii / Acipenser gueldenstaedtii / Acipenser schrenckii / Huso dauricus / Huso huso) . Despite the limitations of mitochondrial DNA in identifying hybrid sturgeon species, the presence of counterfeit caviar of non-sturgeon ingredients could be excluded. All the caviar samples were identified successfully as sturgeon species, but the mislabeling rate of species was 33.4%, indicating that there were illegal phenomena such as disorderly labeling, mislabeling, and adulteration on the market. Article in Journal/Newspaper Acipenser baerii Directory of Open Access Journals: DOAJ Articles Molecules 28 13 5046 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
DNA barcoding caviar COI gene D-loop gene species identification authenticity identification Organic chemistry QD241-441 |
spellingShingle |
DNA barcoding caviar COI gene D-loop gene species identification authenticity identification Organic chemistry QD241-441 Qingqing Hu Yingqiu Pan Huili Xia Kexin Yu Yian Yao Feng Guan Species Identification of Caviar Based on Multiple DNA Barcoding |
topic_facet |
DNA barcoding caviar COI gene D-loop gene species identification authenticity identification Organic chemistry QD241-441 |
description |
This study aimed to explore the applicability of DNA barcoding for assessing the authenticity of caviar on the Chinese market. A set of universal COI primers and two sets of designed primers based on COI and D-loop genes were used to identify maternal species of samples from 21 batches of caviar. The results showed that the PCR products from three sets of primers had more than 98% similarity to the sequences in database. The COI gene could not distinguish sturgeons with closed genetic relationships, while D-loop gene could effectively improve the accuracy of DNA barcoding and was more suitable to the identification of interspecific sturgeon than the COI gene. The neighbor-joining dendrogram further confirmed the applicability and accuracy of COI and D-loop genes in identifying maternal relatives of caviar ( Acipenser baerii / Acipenser gueldenstaedtii / Acipenser schrenckii / Huso dauricus / Huso huso) . Despite the limitations of mitochondrial DNA in identifying hybrid sturgeon species, the presence of counterfeit caviar of non-sturgeon ingredients could be excluded. All the caviar samples were identified successfully as sturgeon species, but the mislabeling rate of species was 33.4%, indicating that there were illegal phenomena such as disorderly labeling, mislabeling, and adulteration on the market. |
format |
Article in Journal/Newspaper |
author |
Qingqing Hu Yingqiu Pan Huili Xia Kexin Yu Yian Yao Feng Guan |
author_facet |
Qingqing Hu Yingqiu Pan Huili Xia Kexin Yu Yian Yao Feng Guan |
author_sort |
Qingqing Hu |
title |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_short |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_full |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_fullStr |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_full_unstemmed |
Species Identification of Caviar Based on Multiple DNA Barcoding |
title_sort |
species identification of caviar based on multiple dna barcoding |
publisher |
MDPI AG |
publishDate |
2023 |
url |
https://doi.org/10.3390/molecules28135046 https://doaj.org/article/3401d9cf31ce4b92897922ff016cf1c9 |
genre |
Acipenser baerii |
genre_facet |
Acipenser baerii |
op_source |
Molecules, Vol 28, Iss 5046, p 5046 (2023) |
op_relation |
https://www.mdpi.com/1420-3049/28/13/5046 https://doaj.org/toc/1420-3049 doi:10.3390/molecules28135046 1420-3049 https://doaj.org/article/3401d9cf31ce4b92897922ff016cf1c9 |
op_doi |
https://doi.org/10.3390/molecules28135046 |
container_title |
Molecules |
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28 |
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13 |
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5046 |
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1772810819933831168 |