Characterization of insecticidal peptides from venom Australian funnel-web spiders
Australian funnel-web spiders are relatively large primitive hunting spiders. Male Atrax robustus spiders have been responsible for a number of human deaths. Venom was collected from the species Hadronyche infensa (Hickman) [female], H. formidabilis [male and female], H. versuta [female], and A. rob...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:33cb496694a045e8af9d83974b01ca02 2023-05-15T15:07:35+02:00 Characterization of insecticidal peptides from venom Australian funnel-web spiders E. J. Vonorax M. I. Tyler R. K. Atkinson M. E. H. Howden 2006-04-01T00:00:00Z https://doi.org/10.1590/S1678-91992006000200005 https://doaj.org/article/33cb496694a045e8af9d83974b01ca02 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200005 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992006000200005 1678-9199 https://doaj.org/article/33cb496694a045e8af9d83974b01ca02 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 2, Pp 215-233 (2006) Australian funnel-web spiders Hadronyche and Atrax species venom insecticidal toxins peptides Helicoverpa armigera Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2006 ftdoajarticles https://doi.org/10.1590/S1678-91992006000200005 2022-12-31T00:14:19Z Australian funnel-web spiders are relatively large primitive hunting spiders. Male Atrax robustus spiders have been responsible for a number of human deaths. Venom was collected from the species Hadronyche infensa (Hickman) [female], H. formidabilis [male and female], H. versuta [female], and A. robustus (Cambridge) [male] and was fractionated by high performance liquid chromatography. This resulted in the isolation and purification of a homologous series of 7 insecticidal peptides of relatively low molecular mass (approximately 4kDa). The amino acid sequences of these toxins consisted of 36 or 37 amino acids and were named atracotoxins. For the major bioassay of these toxins, we used the cotton bollworm, Helicoverpa armigera (Hubner), due to the great damage it causes to crops worldwide. These toxins, when injected subcutaneously into fifth or sixth instar larvae of Helicoverpa armigera,were lethal or caused an apparently irreversible writhing. The toxin from H. versuta venom showed no significant toxicity when subcutaneously injected into newborn mice. One of the toxins was found to have a free acid carboxyl terminus. These toxins have great potential as lead compounds for insecticide design or for incorporation in recombinant baculovirus insecticides. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 12 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Australian funnel-web spiders Hadronyche and Atrax species venom insecticidal toxins peptides Helicoverpa armigera Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Australian funnel-web spiders Hadronyche and Atrax species venom insecticidal toxins peptides Helicoverpa armigera Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 E. J. Vonorax M. I. Tyler R. K. Atkinson M. E. H. Howden Characterization of insecticidal peptides from venom Australian funnel-web spiders |
topic_facet |
Australian funnel-web spiders Hadronyche and Atrax species venom insecticidal toxins peptides Helicoverpa armigera Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Australian funnel-web spiders are relatively large primitive hunting spiders. Male Atrax robustus spiders have been responsible for a number of human deaths. Venom was collected from the species Hadronyche infensa (Hickman) [female], H. formidabilis [male and female], H. versuta [female], and A. robustus (Cambridge) [male] and was fractionated by high performance liquid chromatography. This resulted in the isolation and purification of a homologous series of 7 insecticidal peptides of relatively low molecular mass (approximately 4kDa). The amino acid sequences of these toxins consisted of 36 or 37 amino acids and were named atracotoxins. For the major bioassay of these toxins, we used the cotton bollworm, Helicoverpa armigera (Hubner), due to the great damage it causes to crops worldwide. These toxins, when injected subcutaneously into fifth or sixth instar larvae of Helicoverpa armigera,were lethal or caused an apparently irreversible writhing. The toxin from H. versuta venom showed no significant toxicity when subcutaneously injected into newborn mice. One of the toxins was found to have a free acid carboxyl terminus. These toxins have great potential as lead compounds for insecticide design or for incorporation in recombinant baculovirus insecticides. |
format |
Article in Journal/Newspaper |
author |
E. J. Vonorax M. I. Tyler R. K. Atkinson M. E. H. Howden |
author_facet |
E. J. Vonorax M. I. Tyler R. K. Atkinson M. E. H. Howden |
author_sort |
E. J. Vonorax |
title |
Characterization of insecticidal peptides from venom Australian funnel-web spiders |
title_short |
Characterization of insecticidal peptides from venom Australian funnel-web spiders |
title_full |
Characterization of insecticidal peptides from venom Australian funnel-web spiders |
title_fullStr |
Characterization of insecticidal peptides from venom Australian funnel-web spiders |
title_full_unstemmed |
Characterization of insecticidal peptides from venom Australian funnel-web spiders |
title_sort |
characterization of insecticidal peptides from venom australian funnel-web spiders |
publisher |
SciELO |
publishDate |
2006 |
url |
https://doi.org/10.1590/S1678-91992006000200005 https://doaj.org/article/33cb496694a045e8af9d83974b01ca02 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 12, Iss 2, Pp 215-233 (2006) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992006000200005 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992006000200005 1678-9199 https://doaj.org/article/33cb496694a045e8af9d83974b01ca02 |
op_doi |
https://doi.org/10.1590/S1678-91992006000200005 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
12 |
container_issue |
2 |
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1766339056311992320 |