Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis
Abstract Background Early diagnosis and treatment of malaria are necessary components in the control of malaria. The gold standard light microscopy technique has high sensitivity, but is a relatively time-consuming procedure especially during epidemics and in areas of high endemicity. This study att...
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ftdoajarticles:oai:doaj.org/article:33ba54e0188843fa8915156410c80625 2023-05-15T15:12:32+02:00 Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis Mudathir Mahmoud A Okoued Somia I Hassan Saad El-Din H Malik Elfatih M 2010-03-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-88 https://doaj.org/article/33ba54e0188843fa8915156410c80625 EN eng BMC http://www.malariajournal.com/content/9/1/88 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-88 1475-2875 https://doaj.org/article/33ba54e0188843fa8915156410c80625 Malaria Journal, Vol 9, Iss 1, p 88 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-88 2022-12-31T10:21:14Z Abstract Background Early diagnosis and treatment of malaria are necessary components in the control of malaria. The gold standard light microscopy technique has high sensitivity, but is a relatively time-consuming procedure especially during epidemics and in areas of high endemicity. This study attempted to test the sensitivity and specificity of a new diagnostic tool - the Cyscope ® fluorescence microscope, which is based on the use of Plasmodium nucleic acid-specific fluorescent dyes to facilitate detection of the parasites even in low parasitaemia conditions due to the contrast with the background. Methods In this study, 293 febrile patients above the age of 18 years attending the malaria treatment centre in Sinnar State (Sudan) were interviewed using a structured questionnaire. Finger-prick blood samples were also collected from the participants to be tested for malaria using the hospital's microscope, the reference laboratory microscope, as well as the Cyscope ® microscope. The results of the investigations were then used to calculate the sensitivity, specificity, and positive and negative predictive values of the Cyscope ® microscope in reference to gold standard light microscopy. Results The sensitivity was found to be 98.2% (95% CI: 90.6%-100%); specificity = 98.3% (95% CI: 95.7% - 99.5%); positive predictive value = 93.3% (95% CI: 83.8% - 98.2%); and negative predictive value = 99.6% (95% CI: 97.6% - 100%). Conclusions In conclusion, the Cyscope ® microscope was found to be sensitive, specific and provide rapid, reliable results in a matter of less than 10 minutes. The Cyscope ® microscope should be considered as a viable, cheaper and time-saving option for malaria diagnosis, especially in areas where Plasmodium falciparum is the predominant parasite. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 9 1 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Mudathir Mahmoud A Okoued Somia I Hassan Saad El-Din H Malik Elfatih M Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Early diagnosis and treatment of malaria are necessary components in the control of malaria. The gold standard light microscopy technique has high sensitivity, but is a relatively time-consuming procedure especially during epidemics and in areas of high endemicity. This study attempted to test the sensitivity and specificity of a new diagnostic tool - the Cyscope ® fluorescence microscope, which is based on the use of Plasmodium nucleic acid-specific fluorescent dyes to facilitate detection of the parasites even in low parasitaemia conditions due to the contrast with the background. Methods In this study, 293 febrile patients above the age of 18 years attending the malaria treatment centre in Sinnar State (Sudan) were interviewed using a structured questionnaire. Finger-prick blood samples were also collected from the participants to be tested for malaria using the hospital's microscope, the reference laboratory microscope, as well as the Cyscope ® microscope. The results of the investigations were then used to calculate the sensitivity, specificity, and positive and negative predictive values of the Cyscope ® microscope in reference to gold standard light microscopy. Results The sensitivity was found to be 98.2% (95% CI: 90.6%-100%); specificity = 98.3% (95% CI: 95.7% - 99.5%); positive predictive value = 93.3% (95% CI: 83.8% - 98.2%); and negative predictive value = 99.6% (95% CI: 97.6% - 100%). Conclusions In conclusion, the Cyscope ® microscope was found to be sensitive, specific and provide rapid, reliable results in a matter of less than 10 minutes. The Cyscope ® microscope should be considered as a viable, cheaper and time-saving option for malaria diagnosis, especially in areas where Plasmodium falciparum is the predominant parasite. |
format |
Article in Journal/Newspaper |
author |
Mudathir Mahmoud A Okoued Somia I Hassan Saad El-Din H Malik Elfatih M |
author_facet |
Mudathir Mahmoud A Okoued Somia I Hassan Saad El-Din H Malik Elfatih M |
author_sort |
Mudathir Mahmoud A |
title |
Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis |
title_short |
Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis |
title_full |
Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis |
title_fullStr |
Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis |
title_full_unstemmed |
Testing the sensitivity and specificity of the fluorescence microscope (Cyscope ® ) for malaria diagnosis |
title_sort |
testing the sensitivity and specificity of the fluorescence microscope (cyscope ® ) for malaria diagnosis |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-88 https://doaj.org/article/33ba54e0188843fa8915156410c80625 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 88 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/88 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-88 1475-2875 https://doaj.org/article/33ba54e0188843fa8915156410c80625 |
op_doi |
https://doi.org/10.1186/1475-2875-9-88 |
container_title |
Malaria Journal |
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9 |
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1 |
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1766343205750571008 |