Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes
Objective: To identify the active ingredients, potential targets, and mechanism of Rhizoma coptidis by bioinformatics method, and to explore the hypoglycemic effect of Rhizoma coptidis by in vitro experiments. Methods: The chemical components of Rhizoma coptidis were collected through database searc...
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Wolters Kluwer Medknow Publications
2021
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ftdoajarticles:oai:doaj.org/article:33b0f2d38c0545718df5dd1e78772c44 2023-05-15T15:12:08+02:00 Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes Qian-Qian Zeng Jia-Wei Cai Yue Xu Lin Li Qiu Chen Ren-Song Yue 2021-01-01T00:00:00Z https://doi.org/10.4103/2221-1691.300729 https://doaj.org/article/33b0f2d38c0545718df5dd1e78772c44 EN eng Wolters Kluwer Medknow Publications http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=1;spage=29;epage=39;aulast=Zeng https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.300729 https://doaj.org/article/33b0f2d38c0545718df5dd1e78772c44 Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 1, Pp 29-39 (2021) rhizoma coptidis diabetes network pharmacology target traditional chinese medicine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.4103/2221-1691.300729 2022-12-30T22:07:20Z Objective: To identify the active ingredients, potential targets, and mechanism of Rhizoma coptidis by bioinformatics method, and to explore the hypoglycemic effect of Rhizoma coptidis by in vitro experiments. Methods: The chemical components of Rhizoma coptidis were collected through database search, and oral bioavailability and drug-likeness were used for preliminary screening. The targets of Rhizoma coptidis and diabetes-related targets were collected by database retrieval and reverse docking techniques, and the biological process of cross-set proteins was analyzed. The inhibitory effects of Rhizoma coptidis on α-glucosidase, α-amylase activity, and advanced glycation end products (AGEs) were determined via in vitro experiments. In addition, the effects of Rhizoma coptidis on pre-adipocyte differentiation, absorption of glucose by adipocytes, and the level of intracellular triglyceride were investigated using the adipocyte differentiation model. Results: There were 11 potentially active ingredients in Rhizoma coptidis. IL-6, caspase-3, epidermal growth factor receptor (EGFR), MYC, and estrogen receptor 1 were considered as the key genes. The bioinformatics analysis showed that Rhizoma coptidis played an anti-diabetic role mainly via biological processes and signaling pathways including hormone receptor activity, glutathione binding, steroid binding, etc. In vitro experiments showed that the extract of Rhizoma coptidis inhibited the activities of α-glucosidase and α-amylase, and the generation of AGEs; meanwhile, the extract promoted the absorption of glucose by adipocytes. In addition, the extract of Rhizoma coptidis decreased triglyceride level. Conclusions: Our network pharmacology and in vitro experiments demonstrate the anti-diabetic effects and possible underlying mechanisms of Rhizoma coptidis extract. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 11 1 29 |
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topic |
rhizoma coptidis diabetes network pharmacology target traditional chinese medicine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
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rhizoma coptidis diabetes network pharmacology target traditional chinese medicine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Qian-Qian Zeng Jia-Wei Cai Yue Xu Lin Li Qiu Chen Ren-Song Yue Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes |
topic_facet |
rhizoma coptidis diabetes network pharmacology target traditional chinese medicine Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To identify the active ingredients, potential targets, and mechanism of Rhizoma coptidis by bioinformatics method, and to explore the hypoglycemic effect of Rhizoma coptidis by in vitro experiments. Methods: The chemical components of Rhizoma coptidis were collected through database search, and oral bioavailability and drug-likeness were used for preliminary screening. The targets of Rhizoma coptidis and diabetes-related targets were collected by database retrieval and reverse docking techniques, and the biological process of cross-set proteins was analyzed. The inhibitory effects of Rhizoma coptidis on α-glucosidase, α-amylase activity, and advanced glycation end products (AGEs) were determined via in vitro experiments. In addition, the effects of Rhizoma coptidis on pre-adipocyte differentiation, absorption of glucose by adipocytes, and the level of intracellular triglyceride were investigated using the adipocyte differentiation model. Results: There were 11 potentially active ingredients in Rhizoma coptidis. IL-6, caspase-3, epidermal growth factor receptor (EGFR), MYC, and estrogen receptor 1 were considered as the key genes. The bioinformatics analysis showed that Rhizoma coptidis played an anti-diabetic role mainly via biological processes and signaling pathways including hormone receptor activity, glutathione binding, steroid binding, etc. In vitro experiments showed that the extract of Rhizoma coptidis inhibited the activities of α-glucosidase and α-amylase, and the generation of AGEs; meanwhile, the extract promoted the absorption of glucose by adipocytes. In addition, the extract of Rhizoma coptidis decreased triglyceride level. Conclusions: Our network pharmacology and in vitro experiments demonstrate the anti-diabetic effects and possible underlying mechanisms of Rhizoma coptidis extract. |
format |
Article in Journal/Newspaper |
author |
Qian-Qian Zeng Jia-Wei Cai Yue Xu Lin Li Qiu Chen Ren-Song Yue |
author_facet |
Qian-Qian Zeng Jia-Wei Cai Yue Xu Lin Li Qiu Chen Ren-Song Yue |
author_sort |
Qian-Qian Zeng |
title |
Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes |
title_short |
Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes |
title_full |
Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes |
title_fullStr |
Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes |
title_full_unstemmed |
Network pharmacology-based analysis of effective components and mechanism of Rhizoma coptidis in treating diabetes |
title_sort |
network pharmacology-based analysis of effective components and mechanism of rhizoma coptidis in treating diabetes |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2021 |
url |
https://doi.org/10.4103/2221-1691.300729 https://doaj.org/article/33b0f2d38c0545718df5dd1e78772c44 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 11, Iss 1, Pp 29-39 (2021) |
op_relation |
http://www.apjtb.org/article.asp?issn=2221-1691;year=2021;volume=11;issue=1;spage=29;epage=39;aulast=Zeng https://doaj.org/toc/2221-1691 https://doaj.org/toc/2588-9222 2221-1691 2588-9222 doi:10.4103/2221-1691.300729 https://doaj.org/article/33b0f2d38c0545718df5dd1e78772c44 |
op_doi |
https://doi.org/10.4103/2221-1691.300729 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
11 |
container_issue |
1 |
container_start_page |
29 |
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1766342857744973824 |