Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula.
Schistosome infection begins with the penetration of cercariae through healthy unbroken host skin. This process leads to the transformation of the free-living larvae into obligate parasites called schistosomula. This irreversible transformation, which occurs in as little as two hours, involves casti...
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ftdoajarticles:oai:doaj.org/article:339b5be85f9c4f3c8c4cf3ca1875fad7 2023-05-15T15:12:42+02:00 Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula. Anna V Protasio David W Dunne Matthew Berriman 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002091 https://doaj.org/article/339b5be85f9c4f3c8c4cf3ca1875fad7 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3597483?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002091 https://doaj.org/article/339b5be85f9c4f3c8c4cf3ca1875fad7 PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2091 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002091 2022-12-31T11:12:40Z Schistosome infection begins with the penetration of cercariae through healthy unbroken host skin. This process leads to the transformation of the free-living larvae into obligate parasites called schistosomula. This irreversible transformation, which occurs in as little as two hours, involves casting the cercaria tail and complete remodelling of the surface membrane. At this stage, parasites are vulnerable to host immune attack and oxidative stress. Consequently, the mechanisms by which the parasite recognises and swiftly adapts to the human host are still the subject of many studies, especially in the context of development of intervention strategies against schistosomiasis infection. Because obtaining enough material from in vivo infections is not always feasible for such studies, the transformation process is often mimicked in the laboratory by application of shear pressure to a cercarial sample resulting in mechanically transformed (MT) schistosomula. These parasites share remarkable morphological and biochemical similarity to the naturally transformed counterparts and have been considered a good proxy for parasites undergoing natural infection. Relying on this equivalency, MT schistosomula have been used almost exclusively in high-throughput studies of gene expression, identification of drug targets and identification of effective drugs against schistosomes. However, the transcriptional equivalency between skin-transformed (ST) and MT schistosomula has never been proven. In our approach to compare these two types of schistosomula preparations and to explore differences in gene expression triggered by the presence of a skin barrier, we performed RNA-seq transcriptome profiling of ST and MT schistosomula at 24 hours post transformation. We report that these two very distinct schistosomula preparations differ only in the expression of 38 genes (out of ∼11,000), providing convincing evidence to resolve the skin vs. mechanical long-lasting controversy. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 3 e2091 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Anna V Protasio David W Dunne Matthew Berriman Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Schistosome infection begins with the penetration of cercariae through healthy unbroken host skin. This process leads to the transformation of the free-living larvae into obligate parasites called schistosomula. This irreversible transformation, which occurs in as little as two hours, involves casting the cercaria tail and complete remodelling of the surface membrane. At this stage, parasites are vulnerable to host immune attack and oxidative stress. Consequently, the mechanisms by which the parasite recognises and swiftly adapts to the human host are still the subject of many studies, especially in the context of development of intervention strategies against schistosomiasis infection. Because obtaining enough material from in vivo infections is not always feasible for such studies, the transformation process is often mimicked in the laboratory by application of shear pressure to a cercarial sample resulting in mechanically transformed (MT) schistosomula. These parasites share remarkable morphological and biochemical similarity to the naturally transformed counterparts and have been considered a good proxy for parasites undergoing natural infection. Relying on this equivalency, MT schistosomula have been used almost exclusively in high-throughput studies of gene expression, identification of drug targets and identification of effective drugs against schistosomes. However, the transcriptional equivalency between skin-transformed (ST) and MT schistosomula has never been proven. In our approach to compare these two types of schistosomula preparations and to explore differences in gene expression triggered by the presence of a skin barrier, we performed RNA-seq transcriptome profiling of ST and MT schistosomula at 24 hours post transformation. We report that these two very distinct schistosomula preparations differ only in the expression of 38 genes (out of ∼11,000), providing convincing evidence to resolve the skin vs. mechanical long-lasting controversy. |
format |
Article in Journal/Newspaper |
author |
Anna V Protasio David W Dunne Matthew Berriman |
author_facet |
Anna V Protasio David W Dunne Matthew Berriman |
author_sort |
Anna V Protasio |
title |
Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula. |
title_short |
Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula. |
title_full |
Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula. |
title_fullStr |
Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula. |
title_full_unstemmed |
Comparative study of transcriptome profiles of mechanical- and skin-transformed Schistosoma mansoni schistosomula. |
title_sort |
comparative study of transcriptome profiles of mechanical- and skin-transformed schistosoma mansoni schistosomula. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002091 https://doaj.org/article/339b5be85f9c4f3c8c4cf3ca1875fad7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 3, p e2091 (2013) |
op_relation |
http://europepmc.org/articles/PMC3597483?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002091 https://doaj.org/article/339b5be85f9c4f3c8c4cf3ca1875fad7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002091 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
7 |
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3 |
container_start_page |
e2091 |
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