Liverworts frequently form mycothalli on Spitsbergen in the High Arctic
Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes ampli...
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Norwegian Polar Institute
2021
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Online Access: | https://doi.org/10.33265/polar.v40.7727 https://doaj.org/article/338dba295c924a8c98a401634e478a39 |
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ftdoajarticles:oai:doaj.org/article:338dba295c924a8c98a401634e478a39 2023-05-15T14:51:11+02:00 Liverworts frequently form mycothalli on Spitsbergen in the High Arctic Kevin K. Newsham William P. Goodall-Copestake 2021-09-01T00:00:00Z https://doi.org/10.33265/polar.v40.7727 https://doaj.org/article/338dba295c924a8c98a401634e478a39 EN eng Norwegian Polar Institute https://polarresearch.net/index.php/polar/article/view/7727/13865 https://doaj.org/toc/0800-0395 https://doaj.org/toc/1751-8369 0800-0395 1751-8369 doi:10.33265/polar.v40.7727 https://doaj.org/article/338dba295c924a8c98a401634e478a39 Polar Research, Vol 40, Pp 1-13 (2021) edaphic factors jungermanniidae leafy liverworts serendipita svalbard symbiosis Environmental sciences GE1-350 Oceanography GC1-1581 article 2021 ftdoajarticles https://doi.org/10.33265/polar.v40.7727 2022-12-31T04:08:49Z Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes amplified from plant tissues. Microscopy showed that intracellular hyphal coils, key indicators of the symbiosis, were frequent (>40% stem length colonized) in nine species of liverwort in the families Anastrophyllaceae, Lophoziaceae, Cephaloziellaceae, Cephaloziaceae and Scapaniaceae, with hyphae occurring frequently (>40% cells occupied) in the rhizoids of 10 species in the same families. Dark septate hyphae, apparently formed by ascomycetes, were frequent on the stems of members of the Anastrophyllaceae, Cephaloziellaceae and Cephaloziaceae, and typically those growing on acidic mine tailings. Sequencing of fungal rRNA genes showed the presence of nine distinct groups (based on a 3% cut-off for ITS sequence divergence) of the basidiomycete Serendipita in the Anastrophyllaceae and Lophoziaceae, with ordinations and correlative analyses showing the presence of the genus to be positively associated with the frequency of hyphal coils, the occurrence of which was positively associated with edaphic factors (soil δ15N value and concentrations of moisture, nitrogen, carbon and organic matter). We propose that the frequency of mycothalli in leafy liverworts on west Spitsbergen, which is an order of magnitude higher than at lower latitudes, may arise from benefits conferred by mycobionts on their hosts in the harsh environment of the High Arctic. Article in Journal/Newspaper Arctic Polar Research Svalbard Spitsbergen Directory of Open Access Journals: DOAJ Articles Arctic Svalbard Polar Research 40 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
edaphic factors jungermanniidae leafy liverworts serendipita svalbard symbiosis Environmental sciences GE1-350 Oceanography GC1-1581 |
spellingShingle |
edaphic factors jungermanniidae leafy liverworts serendipita svalbard symbiosis Environmental sciences GE1-350 Oceanography GC1-1581 Kevin K. Newsham William P. Goodall-Copestake Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
topic_facet |
edaphic factors jungermanniidae leafy liverworts serendipita svalbard symbiosis Environmental sciences GE1-350 Oceanography GC1-1581 |
description |
Mycothalli, symbioses between liverworts and soil fungi, have not previously been recorded in the Arctic. Here, 13 species of leafy liverwort from west Spitsbergen in the High Arctic are examined for the symbiosis using epifluorescence microscopy and sequencing of fungal ribosomal (r)RNA genes amplified from plant tissues. Microscopy showed that intracellular hyphal coils, key indicators of the symbiosis, were frequent (>40% stem length colonized) in nine species of liverwort in the families Anastrophyllaceae, Lophoziaceae, Cephaloziellaceae, Cephaloziaceae and Scapaniaceae, with hyphae occurring frequently (>40% cells occupied) in the rhizoids of 10 species in the same families. Dark septate hyphae, apparently formed by ascomycetes, were frequent on the stems of members of the Anastrophyllaceae, Cephaloziellaceae and Cephaloziaceae, and typically those growing on acidic mine tailings. Sequencing of fungal rRNA genes showed the presence of nine distinct groups (based on a 3% cut-off for ITS sequence divergence) of the basidiomycete Serendipita in the Anastrophyllaceae and Lophoziaceae, with ordinations and correlative analyses showing the presence of the genus to be positively associated with the frequency of hyphal coils, the occurrence of which was positively associated with edaphic factors (soil δ15N value and concentrations of moisture, nitrogen, carbon and organic matter). We propose that the frequency of mycothalli in leafy liverworts on west Spitsbergen, which is an order of magnitude higher than at lower latitudes, may arise from benefits conferred by mycobionts on their hosts in the harsh environment of the High Arctic. |
format |
Article in Journal/Newspaper |
author |
Kevin K. Newsham William P. Goodall-Copestake |
author_facet |
Kevin K. Newsham William P. Goodall-Copestake |
author_sort |
Kevin K. Newsham |
title |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_short |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_full |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_fullStr |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_full_unstemmed |
Liverworts frequently form mycothalli on Spitsbergen in the High Arctic |
title_sort |
liverworts frequently form mycothalli on spitsbergen in the high arctic |
publisher |
Norwegian Polar Institute |
publishDate |
2021 |
url |
https://doi.org/10.33265/polar.v40.7727 https://doaj.org/article/338dba295c924a8c98a401634e478a39 |
geographic |
Arctic Svalbard |
geographic_facet |
Arctic Svalbard |
genre |
Arctic Polar Research Svalbard Spitsbergen |
genre_facet |
Arctic Polar Research Svalbard Spitsbergen |
op_source |
Polar Research, Vol 40, Pp 1-13 (2021) |
op_relation |
https://polarresearch.net/index.php/polar/article/view/7727/13865 https://doaj.org/toc/0800-0395 https://doaj.org/toc/1751-8369 0800-0395 1751-8369 doi:10.33265/polar.v40.7727 https://doaj.org/article/338dba295c924a8c98a401634e478a39 |
op_doi |
https://doi.org/10.33265/polar.v40.7727 |
container_title |
Polar Research |
container_volume |
40 |
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1766322243539828736 |