Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro
In this study, we isolated Trypanosoma cruzi from chronic Chagas heart disease and from megaesophagus patients. The parasite stock hSLU239 (heart disease) yielded clones h1 and h2, whereas stock mSLU142 (megaesophagus) yielded clones m1, m2, m3 and m4. The parasite growth kinetics, doubling time and...
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1997
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ftdoajarticles:oai:doaj.org/article:3330c0382abe40d384fb5023e337bff1 2023-05-15T15:04:24+02:00 Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro Lauria-Pires L. Santana J.M. Tavares F.S. Teixeira A.R.L. 1997-01-01T00:00:00Z https://doaj.org/article/3330c0382abe40d384fb5023e337bff1 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000300003 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/3330c0382abe40d384fb5023e337bff1 Revista da Sociedade Brasileira de Medicina Tropical, Vol 30, Iss 3, Pp 187-192 (1997) Trypanosoma cruzi Behavioral characterization Genetic diversity Arctic medicine. Tropical medicine RC955-962 article 1997 ftdoajarticles 2022-12-31T03:22:02Z In this study, we isolated Trypanosoma cruzi from chronic Chagas heart disease and from megaesophagus patients. The parasite stock hSLU239 (heart disease) yielded clones h1 and h2, whereas stock mSLU142 (megaesophagus) yielded clones m1, m2, m3 and m4. The parasite growth kinetics, doubling time and differentiation in axenic liquid medium showed broad behavioral diversity. It was shown that a particular pattern of behavior for a parental stock could not necessarily be assigned for subsequent clones. This study indicates that i) each Chagas disease patient is infected with several T. cruzi populations; ii) clonal lines derived from patient samples may have different biological characteristics from the original isolate; and that iii) additional behavioral and/or molecular markers are required for further characterization of Trypanosoma cruzi stocks and clones derived from Chagas disease patients in order to identify correlations with pathology. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Trypanosoma cruzi Behavioral characterization Genetic diversity Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
Trypanosoma cruzi Behavioral characterization Genetic diversity Arctic medicine. Tropical medicine RC955-962 Lauria-Pires L. Santana J.M. Tavares F.S. Teixeira A.R.L. Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro |
topic_facet |
Trypanosoma cruzi Behavioral characterization Genetic diversity Arctic medicine. Tropical medicine RC955-962 |
description |
In this study, we isolated Trypanosoma cruzi from chronic Chagas heart disease and from megaesophagus patients. The parasite stock hSLU239 (heart disease) yielded clones h1 and h2, whereas stock mSLU142 (megaesophagus) yielded clones m1, m2, m3 and m4. The parasite growth kinetics, doubling time and differentiation in axenic liquid medium showed broad behavioral diversity. It was shown that a particular pattern of behavior for a parental stock could not necessarily be assigned for subsequent clones. This study indicates that i) each Chagas disease patient is infected with several T. cruzi populations; ii) clonal lines derived from patient samples may have different biological characteristics from the original isolate; and that iii) additional behavioral and/or molecular markers are required for further characterization of Trypanosoma cruzi stocks and clones derived from Chagas disease patients in order to identify correlations with pathology. |
format |
Article in Journal/Newspaper |
author |
Lauria-Pires L. Santana J.M. Tavares F.S. Teixeira A.R.L. |
author_facet |
Lauria-Pires L. Santana J.M. Tavares F.S. Teixeira A.R.L. |
author_sort |
Lauria-Pires L. |
title |
Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro |
title_short |
Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro |
title_full |
Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro |
title_fullStr |
Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro |
title_full_unstemmed |
Diversity of Trypanosoma cruzi stocks and clones derived from Chagas disease patients: I-Behavioral characterization in vitro |
title_sort |
diversity of trypanosoma cruzi stocks and clones derived from chagas disease patients: i-behavioral characterization in vitro |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
1997 |
url |
https://doaj.org/article/3330c0382abe40d384fb5023e337bff1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 30, Iss 3, Pp 187-192 (1997) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821997000300003 https://doaj.org/toc/0037-8682 https://doaj.org/toc/1678-9849 0037-8682 1678-9849 https://doaj.org/article/3330c0382abe40d384fb5023e337bff1 |
_version_ |
1766336177623793664 |