A highly multiplexed broad pathogen detection assay for infectious disease diagnostics.
Rapid pathogen identification during an acute febrile illness is a critical first step for providing appropriate clinical care and patient isolation. Primary screening using sensitive and specific assays, such as real-time PCR and ELISAs, can rapidly test for known circulating infectious diseases. I...
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ftdoajarticles:oai:doaj.org/article:331216e300594f86a990c0aea6f827ad 2023-05-15T15:08:43+02:00 A highly multiplexed broad pathogen detection assay for infectious disease diagnostics. Jeffrey W Koehler Christina E Douglas Timothy D Minogue 2018-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006889 https://doaj.org/article/331216e300594f86a990c0aea6f827ad EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6245831?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006889 https://doaj.org/article/331216e300594f86a990c0aea6f827ad PLoS Neglected Tropical Diseases, Vol 12, Iss 11, p e0006889 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006889 2022-12-31T00:11:32Z Rapid pathogen identification during an acute febrile illness is a critical first step for providing appropriate clinical care and patient isolation. Primary screening using sensitive and specific assays, such as real-time PCR and ELISAs, can rapidly test for known circulating infectious diseases. If the initial testing is negative, potentially due to a lack of developed diagnostic assays or an incomplete understanding of the pathogens circulating within a geographic region, additional testing would be required including highly multiplexed assays and metagenomic next generation sequencing. To bridge the gap between rapid point of care diagnostics and sequencing, we developed a highly multiplexed assay designed to detect 164 different viruses, bacteria, and parasites using the NanoString nCounter platform. Included in this assay were high consequence pathogens such as Ebola virus, highly endemic organisms including several Plasmodium species, and a large number of less prevalent pathogens to ensure a broad coverage of potential human pathogens. Evaluation of this panel resulted in positive detection of 113 (encompassing 98 different human pathogen types) of the 126 organisms available to us including the medically important Ebola virus, Lassa virus, dengue virus serotypes 1-4, Chikungunya virus, yellow fever virus, and Plasmodium falciparum. Overall, this assay could improve infectious disease diagnostics and biosurveillance efforts as a quick, highly multiplexed, and easy to use pathogen screening tool. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Rapid Point ENVELOPE(-97.552,-97.552,75.868,75.868) PLOS Neglected Tropical Diseases 12 11 e0006889 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jeffrey W Koehler Christina E Douglas Timothy D Minogue A highly multiplexed broad pathogen detection assay for infectious disease diagnostics. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Rapid pathogen identification during an acute febrile illness is a critical first step for providing appropriate clinical care and patient isolation. Primary screening using sensitive and specific assays, such as real-time PCR and ELISAs, can rapidly test for known circulating infectious diseases. If the initial testing is negative, potentially due to a lack of developed diagnostic assays or an incomplete understanding of the pathogens circulating within a geographic region, additional testing would be required including highly multiplexed assays and metagenomic next generation sequencing. To bridge the gap between rapid point of care diagnostics and sequencing, we developed a highly multiplexed assay designed to detect 164 different viruses, bacteria, and parasites using the NanoString nCounter platform. Included in this assay were high consequence pathogens such as Ebola virus, highly endemic organisms including several Plasmodium species, and a large number of less prevalent pathogens to ensure a broad coverage of potential human pathogens. Evaluation of this panel resulted in positive detection of 113 (encompassing 98 different human pathogen types) of the 126 organisms available to us including the medically important Ebola virus, Lassa virus, dengue virus serotypes 1-4, Chikungunya virus, yellow fever virus, and Plasmodium falciparum. Overall, this assay could improve infectious disease diagnostics and biosurveillance efforts as a quick, highly multiplexed, and easy to use pathogen screening tool. |
format |
Article in Journal/Newspaper |
author |
Jeffrey W Koehler Christina E Douglas Timothy D Minogue |
author_facet |
Jeffrey W Koehler Christina E Douglas Timothy D Minogue |
author_sort |
Jeffrey W Koehler |
title |
A highly multiplexed broad pathogen detection assay for infectious disease diagnostics. |
title_short |
A highly multiplexed broad pathogen detection assay for infectious disease diagnostics. |
title_full |
A highly multiplexed broad pathogen detection assay for infectious disease diagnostics. |
title_fullStr |
A highly multiplexed broad pathogen detection assay for infectious disease diagnostics. |
title_full_unstemmed |
A highly multiplexed broad pathogen detection assay for infectious disease diagnostics. |
title_sort |
highly multiplexed broad pathogen detection assay for infectious disease diagnostics. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006889 https://doaj.org/article/331216e300594f86a990c0aea6f827ad |
long_lat |
ENVELOPE(-97.552,-97.552,75.868,75.868) |
geographic |
Arctic Rapid Point |
geographic_facet |
Arctic Rapid Point |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 11, p e0006889 (2018) |
op_relation |
http://europepmc.org/articles/PMC6245831?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006889 https://doaj.org/article/331216e300594f86a990c0aea6f827ad |
op_doi |
https://doi.org/10.1371/journal.pntd.0006889 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
12 |
container_issue |
11 |
container_start_page |
e0006889 |
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1766340027465334784 |