Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry
Objective: To investigate the role of water-soluble extract of Salvia fruticosa (Greek sage) (S. fruticosa) leaves in reducing both intrinsic cellular and H2O2-induced DNA oxidation in cultured human embryonic kidney 293 cells. S. fruticosa, native to the Eastern-Mediterranean basin, is widely used...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:330a3b30662142bcab514b6447926938 2023-05-15T15:10:48+02:00 Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry Saleem Bani Hani Mekki Bayachou 2014-05-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1270 https://doaj.org/article/330a3b30662142bcab514b6447926938 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115303336 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1270 https://doaj.org/article/330a3b30662142bcab514b6447926938 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 5, Pp 399-403 (2014) Salvia fruticosa DNA oxidation Oxidative stress Human embryonic kidney 293 cells Flow cytometry Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1270 2022-12-31T14:29:14Z Objective: To investigate the role of water-soluble extract of Salvia fruticosa (Greek sage) (S. fruticosa) leaves in reducing both intrinsic cellular and H2O2-induced DNA oxidation in cultured human embryonic kidney 293 cells. S. fruticosa, native to the Eastern-Mediterranean basin, is widely used as a medicinal herb for treatment of various diseases. Methods: Dried leaves of S. fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations. Each mL of the preparation contained (7.1×1.0) mg of extract. HEK-293 cells were incubated in one set with S. fruticosa extract in the presence of 0.1 mmol/L H2O2, and in the other set with the addition of the extract alone. The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivatization of 8-oxoguanine moieties. The fluorescence was measured using flow cytometry technique. Results: Cells incubated 3 h with 150 μL extract and exposed to 0.1 mmol/L H2O2 showed lower intensity of fluorescence, and thus lower DNA oxidation. Moreover, cells incubated 3 h with 100 μL of the extract showed lower intensity of fluorescence, and thus lower intrinsic cellular DNA oxidation compared to control (without S. fruticosa). Conclusions: The results from this study suggest that the water-soluble extract of S. fruticosa leaves protects against both H2O2-induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 5 399 403 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Salvia fruticosa DNA oxidation Oxidative stress Human embryonic kidney 293 cells Flow cytometry Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Salvia fruticosa DNA oxidation Oxidative stress Human embryonic kidney 293 cells Flow cytometry Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Saleem Bani Hani Mekki Bayachou Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry |
topic_facet |
Salvia fruticosa DNA oxidation Oxidative stress Human embryonic kidney 293 cells Flow cytometry Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the role of water-soluble extract of Salvia fruticosa (Greek sage) (S. fruticosa) leaves in reducing both intrinsic cellular and H2O2-induced DNA oxidation in cultured human embryonic kidney 293 cells. S. fruticosa, native to the Eastern-Mediterranean basin, is widely used as a medicinal herb for treatment of various diseases. Methods: Dried leaves of S. fruticosa were extracted in phosphate buffer saline and purified using both vacuum and high pressure filtrations. Each mL of the preparation contained (7.1×1.0) mg of extract. HEK-293 cells were incubated in one set with S. fruticosa extract in the presence of 0.1 mmol/L H2O2, and in the other set with the addition of the extract alone. The DNA oxidation was measured using fluorescence upon fluorescein isothiocyanate derivatization of 8-oxoguanine moieties. The fluorescence was measured using flow cytometry technique. Results: Cells incubated 3 h with 150 μL extract and exposed to 0.1 mmol/L H2O2 showed lower intensity of fluorescence, and thus lower DNA oxidation. Moreover, cells incubated 3 h with 100 μL of the extract showed lower intensity of fluorescence, and thus lower intrinsic cellular DNA oxidation compared to control (without S. fruticosa). Conclusions: The results from this study suggest that the water-soluble extract of S. fruticosa leaves protects against both H2O2-induced and intrinsic cellular DNA oxidation in human embryonic kidney 293 cells. |
format |
Article in Journal/Newspaper |
author |
Saleem Bani Hani Mekki Bayachou |
author_facet |
Saleem Bani Hani Mekki Bayachou |
author_sort |
Saleem Bani Hani |
title |
Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry |
title_short |
Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry |
title_full |
Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry |
title_fullStr |
Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry |
title_full_unstemmed |
Salvia fruticosa reduces intrinsic cellular and H2O2-induced DNA oxidation in HEK 293 cells; assessment using flow cytometry |
title_sort |
salvia fruticosa reduces intrinsic cellular and h2o2-induced dna oxidation in hek 293 cells; assessment using flow cytometry |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1270 https://doaj.org/article/330a3b30662142bcab514b6447926938 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss 5, Pp 399-403 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115303336 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1270 https://doaj.org/article/330a3b30662142bcab514b6447926938 |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1270 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_issue |
5 |
container_start_page |
399 |
op_container_end_page |
403 |
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1766341757436428288 |