Detection of circulating polysaccharide antigen of Schistosoma mansoni in hamster sera by crossed immunoelectrophoresis

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Margareth Fernandes, Ione Irulegui, Dirce Mary Correia Lima, Clarice Pires Abrantes, Martha Smith Cressoni, Carlos Henrique Christo, Luiz Caetano da Silva, Clovis Takiguti
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1988
• Subjects: Imunoeletroforese cruzada (IEC); Antígeno circulante anódico (AgCA); Imunocomplexos (IC); Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic
• Online Access: https://doi.org/10.1590/S0036-46651988000200003; https://doaj.org/article/32cdc1e15add49dda791456b7702e4f6

Crossed immunoelectrophoresis (IEC) was used for detection of free and complexed circulating polisaccharide anodic antigen (AgCA) of Schistosoma mansoni in sera of infected hamsters. An attempt was also done to detect AgCA in human sera from patients infected with S. mansoni. The conditions for isolation and detection of complexed AgCA were established. The sensitivity of IEC was increased by incorporation of 2% polyethylene glicol (PEG) to the agarose and by maintaining the system at 4°C during the electrophoretic run. Free AgCA was detected in 12 and the complexed in 30 of the 37 hamsters sera analysed. Correlation between AgCA (free and complexed) and the parasite load was observed. AgCA was not detected, under the experimental conditions used, in human sera from 7 patients in the acute and 23 in the chronic phase of infection.