ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps
In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
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Language: | English |
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:32c6eeaa1d1a4f209c4e830749faad26 2024-09-09T19:28:29+00:00 ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps Paulo Sérgio de Arruda PINTO Adelaide José VAZ Pedro Manuel Leal GERMANO Paulo Mutuko NAKAMURA 2000-04-01T00:00:00Z https://doi.org/10.1590/S0036-46652000000200003 https://doaj.org/article/32c6eeaa1d1a4f209c4e830749faad26 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000200003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652000000200003 0036-4665 1678-9946 https://doaj.org/article/32c6eeaa1d1a4f209c4e830749faad26 Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 2, Pp 71-79 (2000) Swine cysticercosis Immunologic diagnosis Standardization ELISA Taenia crassiceps Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2000 ftdoajarticles https://doi.org/10.1590/S0036-46652000000200003 2024-08-05T17:49:31Z In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from Taenia crassiceps vesicular fluid (VF-Tcra) and crude larvae extract (T-Tcra), and from Taenia solium extracts of scolex (S-Ts) and of larvae (T-Ts). A checkerboard evaluation of antigen, serum and conjugate dilutions, as well as the use of Tween-20 and skim cow milk in wash and blocking solution had a marked effect on improving ELISA performance. All the antigens showed a good performance, but VF-Tcra was the best, with 96.0% and 80.0% sensitivities for cut-offs respectively at 2sd and 3sd, and corresponding specificities of 97.5% and 100.0%. Cross-reactivity was observed only with hydatidosis and ascaridiosis. In view of the high performance observed, the ELISA test should be recommended for the diagnosis of cysticercosis in suspected swine in slaughterhouses and for the screening of cysticercosis in swine production. These results will support integrated measures of cysticercosis control throughout the chain of swine production, effectively contributing to public health. Foi padronizado o teste ELISA para o diagnóstico da cisticercose suína. Após confirmação por exame post-mortem, os soros dos respectivos animais foram empregados como controles positivos e negativos. Soros de suínos portadores de infecções heterólogas foram ensaiados para determinação de reações cruzadas. Os quatro antígenos testados na fase de padronização foram líquido vesicular (VF) e extrato total (T) de larvas de Taenia crassiceps (Tcra) e de extrato de escólex (S) e de cisticercos (T) de Taenia solium (Tso). A titulação em bloco das ótimas concentrações de antígenos e diluições de soros e de conjugado, bem como o emprego de Tween-20 e de leite desnatado nas soluções bloqueadora e de lavagem exerceram nítida influência no desempenho do teste ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 42 2 71 79 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Swine cysticercosis Immunologic diagnosis Standardization ELISA Taenia crassiceps Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Swine cysticercosis Immunologic diagnosis Standardization ELISA Taenia crassiceps Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Paulo Sérgio de Arruda PINTO Adelaide José VAZ Pedro Manuel Leal GERMANO Paulo Mutuko NAKAMURA ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps |
topic_facet |
Swine cysticercosis Immunologic diagnosis Standardization ELISA Taenia crassiceps Antigens Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
In the present study ELISA was standardized for the diagnosis of swine cysticercosis based on necropsy parameters and confirmed positive and negative control sera. Serum samples from pigs with other infections were also assayed to determine possible cross-reactions. Four antigens were assayed: from Taenia crassiceps vesicular fluid (VF-Tcra) and crude larvae extract (T-Tcra), and from Taenia solium extracts of scolex (S-Ts) and of larvae (T-Ts). A checkerboard evaluation of antigen, serum and conjugate dilutions, as well as the use of Tween-20 and skim cow milk in wash and blocking solution had a marked effect on improving ELISA performance. All the antigens showed a good performance, but VF-Tcra was the best, with 96.0% and 80.0% sensitivities for cut-offs respectively at 2sd and 3sd, and corresponding specificities of 97.5% and 100.0%. Cross-reactivity was observed only with hydatidosis and ascaridiosis. In view of the high performance observed, the ELISA test should be recommended for the diagnosis of cysticercosis in suspected swine in slaughterhouses and for the screening of cysticercosis in swine production. These results will support integrated measures of cysticercosis control throughout the chain of swine production, effectively contributing to public health. Foi padronizado o teste ELISA para o diagnóstico da cisticercose suína. Após confirmação por exame post-mortem, os soros dos respectivos animais foram empregados como controles positivos e negativos. Soros de suínos portadores de infecções heterólogas foram ensaiados para determinação de reações cruzadas. Os quatro antígenos testados na fase de padronização foram líquido vesicular (VF) e extrato total (T) de larvas de Taenia crassiceps (Tcra) e de extrato de escólex (S) e de cisticercos (T) de Taenia solium (Tso). A titulação em bloco das ótimas concentrações de antígenos e diluições de soros e de conjugado, bem como o emprego de Tween-20 e de leite desnatado nas soluções bloqueadora e de lavagem exerceram nítida influência no desempenho do teste ... |
format |
Article in Journal/Newspaper |
author |
Paulo Sérgio de Arruda PINTO Adelaide José VAZ Pedro Manuel Leal GERMANO Paulo Mutuko NAKAMURA |
author_facet |
Paulo Sérgio de Arruda PINTO Adelaide José VAZ Pedro Manuel Leal GERMANO Paulo Mutuko NAKAMURA |
author_sort |
Paulo Sérgio de Arruda PINTO |
title |
ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps |
title_short |
ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps |
title_full |
ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps |
title_fullStr |
ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps |
title_full_unstemmed |
ELISA test for the diagnosis of cysticercosis in pigs using antigens of Taenia solium and Taenia crassiceps cysticerci Teste ELISA para diagnóstico da cisticercose suína usando antígenos de larvas de Taenia solium e Taenia crassiceps |
title_sort |
elisa test for the diagnosis of cysticercosis in pigs using antigens of taenia solium and taenia crassiceps cysticerci teste elisa para diagnóstico da cisticercose suína usando antígenos de larvas de taenia solium e taenia crassiceps |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2000 |
url |
https://doi.org/10.1590/S0036-46652000000200003 https://doaj.org/article/32c6eeaa1d1a4f209c4e830749faad26 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 42, Iss 2, Pp 71-79 (2000) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652000000200003 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46652000000200003 0036-4665 1678-9946 https://doaj.org/article/32c6eeaa1d1a4f209c4e830749faad26 |
op_doi |
https://doi.org/10.1590/S0036-46652000000200003 |
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Revista do Instituto de Medicina Tropical de São Paulo |
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42 |
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