A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex.
BACKGROUND:Lutzomyia longipalpis is the South American vector of Leishmania infantum, the etiologic agent of visceral leishmaniasis (VL). Male L. longipalpis produce a sex-aggregation pheromone that is critical in mating, yet very little is known about its accumulation over time or factors involved...
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ftdoajarticles:oai:doaj.org/article:32c60f7e849a423faf60b0cea040f542 2023-05-15T15:17:39+02:00 A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex. Mikel A González Krishna K Bandi Melissa J Bell Reginaldo P Brazil Erin Dilger Angel Guerrero Orin Courtenay James G C Hamilton 2017-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006071 https://doaj.org/article/32c60f7e849a423faf60b0cea040f542 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5745125?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006071 https://doaj.org/article/32c60f7e849a423faf60b0cea040f542 PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006071 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006071 2022-12-31T06:35:59Z BACKGROUND:Lutzomyia longipalpis is the South American vector of Leishmania infantum, the etiologic agent of visceral leishmaniasis (VL). Male L. longipalpis produce a sex-aggregation pheromone that is critical in mating, yet very little is known about its accumulation over time or factors involved in release. This laboratory study aimed to compare accumulation of pheromone over time and determine factors that might influence release in three members of the L. longipalpis species complex. METHODOLOGY/PRINCIPAL FINDINGS:We investigated male sex-aggregation pheromone gland content at different ages and the release rate of pheromone in the presence or absence of females under different light conditions by gas chromatography-mass spectrometry (GC-MS). Pheromone gland content was determined by extraction of whole males and pheromone release rate was determined by collection of headspace volatiles. Pheromone gland content appeared age-related and pheromone began to accumulate between 6 to 12 h post eclosion and gradually increased until males were 7-9 days old. The greatest amount was detected in 9-day old Campo Grande males ((S)-9-methylgermacrene-B; X ± SE: 203.5 ± 57.4 ng/male) followed by Sobral 2S males (diterpene; 199.9 ± 34.3) and Jacobina males ((1S,3S,7R)-3-methyl-α-himachalene; 128.8 ± 30.3) at 7 days old. Pheromone release was not continuous over time. During a 4-hour period, the greatest quantities of pheromone were released during the first hour, when wing beating activity was most intense. It was then substantially diminished for the remainder of the time. During a 24 h period, 4-5 day old male sand flies released approximately 63 ± 11% of the pheromone content of their glands, depending on the chemotype. The presence of females significantly increased pheromone release rate. The light regime under which the sand flies were held had little influence on pheromone release except on Sobral 2S chemotype. CONCLUSIONS/SIGNIFICANCE:Accumulation of pheromone appears to occur at different rates in the different ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Sobral ENVELOPE(-40.650,-40.650,-81.083,-81.083) PLOS Neglected Tropical Diseases 11 12 e0006071 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Mikel A González Krishna K Bandi Melissa J Bell Reginaldo P Brazil Erin Dilger Angel Guerrero Orin Courtenay James G C Hamilton A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Lutzomyia longipalpis is the South American vector of Leishmania infantum, the etiologic agent of visceral leishmaniasis (VL). Male L. longipalpis produce a sex-aggregation pheromone that is critical in mating, yet very little is known about its accumulation over time or factors involved in release. This laboratory study aimed to compare accumulation of pheromone over time and determine factors that might influence release in three members of the L. longipalpis species complex. METHODOLOGY/PRINCIPAL FINDINGS:We investigated male sex-aggregation pheromone gland content at different ages and the release rate of pheromone in the presence or absence of females under different light conditions by gas chromatography-mass spectrometry (GC-MS). Pheromone gland content was determined by extraction of whole males and pheromone release rate was determined by collection of headspace volatiles. Pheromone gland content appeared age-related and pheromone began to accumulate between 6 to 12 h post eclosion and gradually increased until males were 7-9 days old. The greatest amount was detected in 9-day old Campo Grande males ((S)-9-methylgermacrene-B; X ± SE: 203.5 ± 57.4 ng/male) followed by Sobral 2S males (diterpene; 199.9 ± 34.3) and Jacobina males ((1S,3S,7R)-3-methyl-α-himachalene; 128.8 ± 30.3) at 7 days old. Pheromone release was not continuous over time. During a 4-hour period, the greatest quantities of pheromone were released during the first hour, when wing beating activity was most intense. It was then substantially diminished for the remainder of the time. During a 24 h period, 4-5 day old male sand flies released approximately 63 ± 11% of the pheromone content of their glands, depending on the chemotype. The presence of females significantly increased pheromone release rate. The light regime under which the sand flies were held had little influence on pheromone release except on Sobral 2S chemotype. CONCLUSIONS/SIGNIFICANCE:Accumulation of pheromone appears to occur at different rates in the different ... |
format |
Article in Journal/Newspaper |
author |
Mikel A González Krishna K Bandi Melissa J Bell Reginaldo P Brazil Erin Dilger Angel Guerrero Orin Courtenay James G C Hamilton |
author_facet |
Mikel A González Krishna K Bandi Melissa J Bell Reginaldo P Brazil Erin Dilger Angel Guerrero Orin Courtenay James G C Hamilton |
author_sort |
Mikel A González |
title |
A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex. |
title_short |
A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex. |
title_full |
A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex. |
title_fullStr |
A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex. |
title_full_unstemmed |
A temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the Lutzomyia longipalpis (Diptera: Psychodidae) species complex. |
title_sort |
temporal comparison of sex-aggregation pheromone gland content and dynamics of release in three members of the lutzomyia longipalpis (diptera: psychodidae) species complex. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0006071 https://doaj.org/article/32c60f7e849a423faf60b0cea040f542 |
long_lat |
ENVELOPE(-40.650,-40.650,-81.083,-81.083) |
geographic |
Arctic Sobral |
geographic_facet |
Arctic Sobral |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 12, p e0006071 (2017) |
op_relation |
http://europepmc.org/articles/PMC5745125?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006071 https://doaj.org/article/32c60f7e849a423faf60b0cea040f542 |
op_doi |
https://doi.org/10.1371/journal.pntd.0006071 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
11 |
container_issue |
12 |
container_start_page |
e0006071 |
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