Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and s...
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ftdoajarticles:oai:doaj.org/article:328243065cd246bfaaab7660e3c1cede 2023-05-15T17:12:37+02:00 Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson 2021-08-01T00:00:00Z https://doi.org/10.3390/v13081640 https://doaj.org/article/328243065cd246bfaaab7660e3c1cede EN eng MDPI AG https://www.mdpi.com/1999-4915/13/8/1640 https://doaj.org/toc/1999-4915 doi:10.3390/v13081640 1999-4915 https://doaj.org/article/328243065cd246bfaaab7660e3c1cede Viruses, Vol 13, Iss 1640, p 1640 (2021) Puumala orthohantavirus Ukraine next generation sequencing field survey Microbiology QR1-502 article 2021 ftdoajarticles https://doi.org/10.3390/v13081640 2022-12-31T13:00:28Z In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and strains of hantaviruses circulating in this region. We conducted surveillance of small rodent populations in a rural region in northwestern Ukraine approximately 30 km from Poland. From the 424 small mammals captured, we identified nine species, of which the most abundant were Myodes glareolus , the bank vole (45%); Apodemus flavicollis , the yellow-necked mouse (29%); and Apodemus agrarius , the striped field mouse (14.6%) Using an indirect immunofluorescence assay, 15.7%, 20.5%, and 33.9% of the sera from M. glareolus , A. glareolus , and A. flavicollis were positive for hantaviral antibodies, respectively. Additionally, we detected antibodies to the hantaviral antigen in one Microtus arvalis , one Mus musculus , and one Sorex minutus . We screened the lung tissue for hantaviral RNA using next-generation sequencing and identified PUUV sequences in 25 small mammals, including 23 M. glareolus , 1 M. musculus , and 1 A. flavicollis, but we were unable to detect DOBV sequences in any of our A. agrarius specimens. The percent identity matrix and Bayesian phylogenetic analyses of the S-segment of PUUV from 14 M. glareolus lungs suggest the highest similarity (92–95% nucleotide or 99–100% amino acid) with the Latvian lineage. This new genetic information will contribute to future molecular surveillance of human cases in Ukraine. Article in Journal/Newspaper Microtus arvalis Directory of Open Access Journals: DOAJ Articles Viruses 13 8 1640 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Puumala orthohantavirus Ukraine next generation sequencing field survey Microbiology QR1-502 |
spellingShingle |
Puumala orthohantavirus Ukraine next generation sequencing field survey Microbiology QR1-502 Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
topic_facet |
Puumala orthohantavirus Ukraine next generation sequencing field survey Microbiology QR1-502 |
description |
In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and strains of hantaviruses circulating in this region. We conducted surveillance of small rodent populations in a rural region in northwestern Ukraine approximately 30 km from Poland. From the 424 small mammals captured, we identified nine species, of which the most abundant were Myodes glareolus , the bank vole (45%); Apodemus flavicollis , the yellow-necked mouse (29%); and Apodemus agrarius , the striped field mouse (14.6%) Using an indirect immunofluorescence assay, 15.7%, 20.5%, and 33.9% of the sera from M. glareolus , A. glareolus , and A. flavicollis were positive for hantaviral antibodies, respectively. Additionally, we detected antibodies to the hantaviral antigen in one Microtus arvalis , one Mus musculus , and one Sorex minutus . We screened the lung tissue for hantaviral RNA using next-generation sequencing and identified PUUV sequences in 25 small mammals, including 23 M. glareolus , 1 M. musculus , and 1 A. flavicollis, but we were unable to detect DOBV sequences in any of our A. agrarius specimens. The percent identity matrix and Bayesian phylogenetic analyses of the S-segment of PUUV from 14 M. glareolus lungs suggest the highest similarity (92–95% nucleotide or 99–100% amino acid) with the Latvian lineage. This new genetic information will contribute to future molecular surveillance of human cases in Ukraine. |
format |
Article in Journal/Newspaper |
author |
Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson |
author_facet |
Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson |
author_sort |
Evan P. Williams |
title |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_short |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_full |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_fullStr |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_full_unstemmed |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_sort |
prevalence of hantaviruses harbored by murid rodents in northwestern ukraine and discovery of a novel puumala virus strain |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/v13081640 https://doaj.org/article/328243065cd246bfaaab7660e3c1cede |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Viruses, Vol 13, Iss 1640, p 1640 (2021) |
op_relation |
https://www.mdpi.com/1999-4915/13/8/1640 https://doaj.org/toc/1999-4915 doi:10.3390/v13081640 1999-4915 https://doaj.org/article/328243065cd246bfaaab7660e3c1cede |
op_doi |
https://doi.org/10.3390/v13081640 |
container_title |
Viruses |
container_volume |
13 |
container_issue |
8 |
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1640 |
_version_ |
1766069407370444800 |