Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra
Progesterone-induced obesity develops in women who use this drug for contraception and the menopause treatment, though its mechanisms remain poorly understood. We studied functional M1 and M2 polarizations of the abdominal cavity macrophages of rats with progesterone induced obesity during 28 days o...
Published in: | Regulatory Mechanisms in Biosystems |
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Oles Honchar Dnipro National University
2019
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ftdoajarticles:oai:doaj.org/article:324e45d1e6d34117a1f7d9537849a9b8 2023-05-15T13:42:23+02:00 Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra V. I. Konopelnuk I. V. Kompanets V. M. Svyatetska O. S. Molozhavaya L. I. Ostapchenko 2019-11-01T00:00:00Z https://doi.org/10.15421/021979 https://doaj.org/article/324e45d1e6d34117a1f7d9537849a9b8 EN eng Oles Honchar Dnipro National University https://medicine.dp.ua/index.php/med/article/view/579 https://doaj.org/toc/2519-8521 https://doaj.org/toc/2520-2588 2519-8521 2520-2588 doi:10.15421/021979 https://doaj.org/article/324e45d1e6d34117a1f7d9537849a9b8 Regulatory Mechanisms in Biosystems, Vol 10, Iss 4, Pp 538-543 (2019) progesterone obesity melanin peritoneal macrophages m1 and m2 polarization Science Q article 2019 ftdoajarticles https://doi.org/10.15421/021979 2022-12-30T19:39:23Z Progesterone-induced obesity develops in women who use this drug for contraception and the menopause treatment, though its mechanisms remain poorly understood. We studied functional M1 and M2 polarizations of the abdominal cavity macrophages of rats with progesterone induced obesity during 28 days of administration. The effect of melanin from the Antarctic yeast Nadsoniella nigra (Chaetothyriales, Herpotrichiellaceae, Nadsoniella Issatsch, 1914) was investigated. The NO level was determined by the accumulation of nitrites, ROS level was estimated by the NBT-test, arginase activity was assayed by the reaction of L-arginine hydrolysis. The body weights of rats administrated progesterone increased by 27% and continued to increase one month after withdrawal of progesterone (55% higher than control). Melanin prevents the weight gain when administered during one month after progesterone withdrawal. The NO production by peritoneal macrophages of obese animals intensified by 31% indicating their polarization towards pro-inflammatory M1 type. Production of ROS did not change. A 14% increase in arginase activity was observed, indicating the inhibition of M2 (anti-inflammatory) polarization. In the progesterone withdrawal group all these rates significantly decreased, indicating a reduction in the functional activity of peritoneal macrophages’. Melanin decreased the NO and ROS production by 60% and 18% respectively in comparison with the progesterone group and unexpectedly reduced arginase activity. Our data provide evidence of the spread of inflammation in response to progesterone-induced obesity. Peritoneal macrophages are involved in the inflammation in obesity, undergoing polarization towards the pro-inflammatory phenotype. The long-term consequences of such inflammation include the continuation of weight gain and likely the development of systemic inflammation associated with the exhaustion of the functional capacity of peritoneal cavity macrophages. Melanin has an anti-obesity effect and exhibits anti-inflammatory ... Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Regulatory Mechanisms in Biosystems 10 4 538 543 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
progesterone obesity melanin peritoneal macrophages m1 and m2 polarization Science Q |
spellingShingle |
progesterone obesity melanin peritoneal macrophages m1 and m2 polarization Science Q V. I. Konopelnuk I. V. Kompanets V. M. Svyatetska O. S. Molozhavaya L. I. Ostapchenko Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra |
topic_facet |
progesterone obesity melanin peritoneal macrophages m1 and m2 polarization Science Q |
description |
Progesterone-induced obesity develops in women who use this drug for contraception and the menopause treatment, though its mechanisms remain poorly understood. We studied functional M1 and M2 polarizations of the abdominal cavity macrophages of rats with progesterone induced obesity during 28 days of administration. The effect of melanin from the Antarctic yeast Nadsoniella nigra (Chaetothyriales, Herpotrichiellaceae, Nadsoniella Issatsch, 1914) was investigated. The NO level was determined by the accumulation of nitrites, ROS level was estimated by the NBT-test, arginase activity was assayed by the reaction of L-arginine hydrolysis. The body weights of rats administrated progesterone increased by 27% and continued to increase one month after withdrawal of progesterone (55% higher than control). Melanin prevents the weight gain when administered during one month after progesterone withdrawal. The NO production by peritoneal macrophages of obese animals intensified by 31% indicating their polarization towards pro-inflammatory M1 type. Production of ROS did not change. A 14% increase in arginase activity was observed, indicating the inhibition of M2 (anti-inflammatory) polarization. In the progesterone withdrawal group all these rates significantly decreased, indicating a reduction in the functional activity of peritoneal macrophages’. Melanin decreased the NO and ROS production by 60% and 18% respectively in comparison with the progesterone group and unexpectedly reduced arginase activity. Our data provide evidence of the spread of inflammation in response to progesterone-induced obesity. Peritoneal macrophages are involved in the inflammation in obesity, undergoing polarization towards the pro-inflammatory phenotype. The long-term consequences of such inflammation include the continuation of weight gain and likely the development of systemic inflammation associated with the exhaustion of the functional capacity of peritoneal cavity macrophages. Melanin has an anti-obesity effect and exhibits anti-inflammatory ... |
format |
Article in Journal/Newspaper |
author |
V. I. Konopelnuk I. V. Kompanets V. M. Svyatetska O. S. Molozhavaya L. I. Ostapchenko |
author_facet |
V. I. Konopelnuk I. V. Kompanets V. M. Svyatetska O. S. Molozhavaya L. I. Ostapchenko |
author_sort |
V. I. Konopelnuk |
title |
Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra |
title_short |
Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra |
title_full |
Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra |
title_fullStr |
Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra |
title_full_unstemmed |
Functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the Antarctic yeast Nadsoniella nigra |
title_sort |
functional polarization of macrophages of rats with progesterone-induced obesity treated with melanin from the antarctic yeast nadsoniella nigra |
publisher |
Oles Honchar Dnipro National University |
publishDate |
2019 |
url |
https://doi.org/10.15421/021979 https://doaj.org/article/324e45d1e6d34117a1f7d9537849a9b8 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Regulatory Mechanisms in Biosystems, Vol 10, Iss 4, Pp 538-543 (2019) |
op_relation |
https://medicine.dp.ua/index.php/med/article/view/579 https://doaj.org/toc/2519-8521 https://doaj.org/toc/2520-2588 2519-8521 2520-2588 doi:10.15421/021979 https://doaj.org/article/324e45d1e6d34117a1f7d9537849a9b8 |
op_doi |
https://doi.org/10.15421/021979 |
container_title |
Regulatory Mechanisms in Biosystems |
container_volume |
10 |
container_issue |
4 |
container_start_page |
538 |
op_container_end_page |
543 |
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1766167456808697856 |