Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations
ABSTRACT In Brazil, few studies on the molecular aspects of hepatitis B virus (HBV) infection have been conducted in the interior regions of Sao Paulo State. This study aimed to identify HBV genotypes and evaluate strains with resistance mutations for nucleoside analogues in the Administrative Regio...
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Universidade de São Paulo (USP)
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ftdoajarticles:oai:doaj.org/article:321f9fe3ef5f420b81ac837c48888962 2024-09-09T19:26:32+00:00 Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations Brígida Helena da Silva Meneghello Márcia Maria Costa Nunes Soares Vanessa Cristina Martins Silva Marcilio Figueiredo Lemos Murilo Castro Cervato João Caetano Filho Roberta Sitnik Tânia Cristina Higino Estécio Adriana Parise Compri João Renato Rebello Pinho Regina Célia Moreira 2021-11-01T00:00:00Z https://doi.org/10.1590/s1678-9946202163078 https://doaj.org/article/321f9fe3ef5f420b81ac837c48888962 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100245&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163078 https://doaj.org/article/321f9fe3ef5f420b81ac837c48888962 Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) Hepatitis B virus HBV genotypes Resistant mutations Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1590/s1678-9946202163078 2024-08-05T17:49:31Z ABSTRACT In Brazil, few studies on the molecular aspects of hepatitis B virus (HBV) infection have been conducted in the interior regions of Sao Paulo State. This study aimed to identify HBV genotypes and evaluate strains with resistance mutations for nucleoside analogues in the Administrative Region (AR) of the municipality of Sao Jose do Rio Preto. We performed nested PCRs of 127 samples from the Health Care Services of the AR to amplify, sequence and analyze fragments of the HBV DNA, in order to identify genotypes and resistance mutations. The HBV S/Pol regions of 126 samples were successfully amplified and sequenced. Five different genotypes were found, and the main ones were A, D and F; a greater number of samples contained the subgenotypes A1 (n = 51; 40.5%), D3 (n = 36; 28.6%), A2 (n = 14; 11.1%) and F2a (n = 9; 7.1%). Resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV173L) were identified in 13.9% (5/36) of patients undergoing viral treatment and 1.1% (1/90) of naïve patients. The diversity of genotypes/subgenotypes found is probably due to the intense migration occurring in the region. These data can complement epidemiological and clinical surveillance, and can be used for a more effective management of chronic HBV patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista do Instituto de Medicina Tropical de São Paulo 63 |
institution |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Hepatitis B virus HBV genotypes Resistant mutations Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Hepatitis B virus HBV genotypes Resistant mutations Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Brígida Helena da Silva Meneghello Márcia Maria Costa Nunes Soares Vanessa Cristina Martins Silva Marcilio Figueiredo Lemos Murilo Castro Cervato João Caetano Filho Roberta Sitnik Tânia Cristina Higino Estécio Adriana Parise Compri João Renato Rebello Pinho Regina Célia Moreira Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations |
topic_facet |
Hepatitis B virus HBV genotypes Resistant mutations Brazil Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
ABSTRACT In Brazil, few studies on the molecular aspects of hepatitis B virus (HBV) infection have been conducted in the interior regions of Sao Paulo State. This study aimed to identify HBV genotypes and evaluate strains with resistance mutations for nucleoside analogues in the Administrative Region (AR) of the municipality of Sao Jose do Rio Preto. We performed nested PCRs of 127 samples from the Health Care Services of the AR to amplify, sequence and analyze fragments of the HBV DNA, in order to identify genotypes and resistance mutations. The HBV S/Pol regions of 126 samples were successfully amplified and sequenced. Five different genotypes were found, and the main ones were A, D and F; a greater number of samples contained the subgenotypes A1 (n = 51; 40.5%), D3 (n = 36; 28.6%), A2 (n = 14; 11.1%) and F2a (n = 9; 7.1%). Resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV173L) were identified in 13.9% (5/36) of patients undergoing viral treatment and 1.1% (1/90) of naïve patients. The diversity of genotypes/subgenotypes found is probably due to the intense migration occurring in the region. These data can complement epidemiological and clinical surveillance, and can be used for a more effective management of chronic HBV patients. |
format |
Article in Journal/Newspaper |
author |
Brígida Helena da Silva Meneghello Márcia Maria Costa Nunes Soares Vanessa Cristina Martins Silva Marcilio Figueiredo Lemos Murilo Castro Cervato João Caetano Filho Roberta Sitnik Tânia Cristina Higino Estécio Adriana Parise Compri João Renato Rebello Pinho Regina Célia Moreira |
author_facet |
Brígida Helena da Silva Meneghello Márcia Maria Costa Nunes Soares Vanessa Cristina Martins Silva Marcilio Figueiredo Lemos Murilo Castro Cervato João Caetano Filho Roberta Sitnik Tânia Cristina Higino Estécio Adriana Parise Compri João Renato Rebello Pinho Regina Célia Moreira |
author_sort |
Brígida Helena da Silva Meneghello |
title |
Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations |
title_short |
Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations |
title_full |
Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations |
title_fullStr |
Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations |
title_full_unstemmed |
Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations |
title_sort |
hepatitis b in the northwestern region of sao paulo state: genotypes and resistance mutations |
publisher |
Universidade de São Paulo (USP) |
publishDate |
2021 |
url |
https://doi.org/10.1590/s1678-9946202163078 https://doaj.org/article/321f9fe3ef5f420b81ac837c48888962 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 63 (2021) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652021000100245&tlng=en https://doaj.org/toc/1678-9946 1678-9946 doi:10.1590/s1678-9946202163078 https://doaj.org/article/321f9fe3ef5f420b81ac837c48888962 |
op_doi |
https://doi.org/10.1590/s1678-9946202163078 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
63 |
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1809896120300601344 |