A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L.
Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny...
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ftdoajarticles:oai:doaj.org/article:320f1377534f462d806f425d2866f496 2023-05-15T17:43:30+02:00 A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. Anton Liaimer John Beck Jensen Elke Dittmann 2016-11-01T00:00:00Z https://doi.org/10.3389/fmicb.2016.01693 https://doaj.org/article/320f1377534f462d806f425d2866f496 EN eng Frontiers Media S.A. http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01693/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.01693 https://doaj.org/article/320f1377534f462d806f425d2866f496 Frontiers in Microbiology, Vol 7 (2016) Nostoc Symbiosis Allelopathy secondary metabolites : cyanobacteria Blasia Microbiology QR1-502 article 2016 ftdoajarticles https://doi.org/10.3389/fmicb.2016.01693 2022-12-31T00:23:59Z Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction showed a remarkable local diversity among isolates assigned to several Nostoc clades. An extensive web of negative allelopathic interactions was recorded at an agricultural site, but not at the undisturbed natural site. The cell extracts of the cyanobacteria did not show antimicrobial activities, but four isolates were shown to be cytotoxic to human cells. The secondary metabolite profiles of the isolates were mapped by MALDI-TOF MS, and the most prominent ions were further analysed by Q-TOF for MS/MS aided identification. Symbiotic isolates produced a great variety of small peptide-like substances, most of which lack any record in the databases. Among identified compounds we found microcystin and nodularin variants toxic to eukaryotic cells. Microcystin producing chemotypes were dominating as symbiotic recruits but not in the free-living community. In addition, we were able to identify several novel aeruginosins and banyaside-like compounds, as well as nostocyclopeptides and nosperin. Article in Journal/Newspaper Northern Norway Directory of Open Access Journals: DOAJ Articles Norway Frontiers in Microbiology 7 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Nostoc Symbiosis Allelopathy secondary metabolites : cyanobacteria Blasia Microbiology QR1-502 |
spellingShingle |
Nostoc Symbiosis Allelopathy secondary metabolites : cyanobacteria Blasia Microbiology QR1-502 Anton Liaimer John Beck Jensen Elke Dittmann A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
topic_facet |
Nostoc Symbiosis Allelopathy secondary metabolites : cyanobacteria Blasia Microbiology QR1-502 |
description |
Liverwort Blasia pusilla L. recruits soil nitrogen-fixing cyanobacteria of genus Nostoc as symbiotic partners. In this work we compared Nostoc community composition inside the plants and in the soil around them from two distant locations in Northern Norway. STRR fingerprinting and 16S rDNA phylogeny reconstruction showed a remarkable local diversity among isolates assigned to several Nostoc clades. An extensive web of negative allelopathic interactions was recorded at an agricultural site, but not at the undisturbed natural site. The cell extracts of the cyanobacteria did not show antimicrobial activities, but four isolates were shown to be cytotoxic to human cells. The secondary metabolite profiles of the isolates were mapped by MALDI-TOF MS, and the most prominent ions were further analysed by Q-TOF for MS/MS aided identification. Symbiotic isolates produced a great variety of small peptide-like substances, most of which lack any record in the databases. Among identified compounds we found microcystin and nodularin variants toxic to eukaryotic cells. Microcystin producing chemotypes were dominating as symbiotic recruits but not in the free-living community. In addition, we were able to identify several novel aeruginosins and banyaside-like compounds, as well as nostocyclopeptides and nosperin. |
format |
Article in Journal/Newspaper |
author |
Anton Liaimer John Beck Jensen Elke Dittmann |
author_facet |
Anton Liaimer John Beck Jensen Elke Dittmann |
author_sort |
Anton Liaimer |
title |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_short |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_full |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_fullStr |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_full_unstemmed |
A Genetic and Chemical Perspective on Symbiotic Recruitment of Cyanobacteria of the Genus Nostoc into the Host Plant Blasia pusilla L. |
title_sort |
genetic and chemical perspective on symbiotic recruitment of cyanobacteria of the genus nostoc into the host plant blasia pusilla l. |
publisher |
Frontiers Media S.A. |
publishDate |
2016 |
url |
https://doi.org/10.3389/fmicb.2016.01693 https://doaj.org/article/320f1377534f462d806f425d2866f496 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Northern Norway |
genre_facet |
Northern Norway |
op_source |
Frontiers in Microbiology, Vol 7 (2016) |
op_relation |
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.01693/full https://doaj.org/toc/1664-302X 1664-302X doi:10.3389/fmicb.2016.01693 https://doaj.org/article/320f1377534f462d806f425d2866f496 |
op_doi |
https://doi.org/10.3389/fmicb.2016.01693 |
container_title |
Frontiers in Microbiology |
container_volume |
7 |
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1766145603191963648 |