Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease.
Chagas disease (CD), caused by the parasite Trypanosoma cruzi, is one of nineteen neglected tropical diseases. CD is a vector-borne disease transmitted by triatomines, but CD can also be transmitted through blood transfusions, organ transplants, T. cruzi-contaminated food and drinks, and congenital...
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ftdoajarticles:oai:doaj.org/article:320ec1dc96c94dfa9fe6f452cffc88d9 2023-05-15T15:08:33+02:00 Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease. Danya A Dean Gautham Gautham Jair L Siqueira-Neto James H McKerrow Pieter C Dorrestein Laura-Isobel McCall 2021-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009819 https://doaj.org/article/320ec1dc96c94dfa9fe6f452cffc88d9 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009819 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009819 https://doaj.org/article/320ec1dc96c94dfa9fe6f452cffc88d9 PLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e0009819 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009819 2022-12-30T21:48:57Z Chagas disease (CD), caused by the parasite Trypanosoma cruzi, is one of nineteen neglected tropical diseases. CD is a vector-borne disease transmitted by triatomines, but CD can also be transmitted through blood transfusions, organ transplants, T. cruzi-contaminated food and drinks, and congenital transmission. While endemic to the Americas, T. cruzi infects 7-8 million people worldwide and can induce severe cardiac symptoms including apical aneurysms, thromboembolisms and arrhythmias during the chronic stage of CD. However, these cardiac clinical manifestations and CD pathogenesis are not fully understood. Using spatial metabolomics (chemical cartography), we sought to understand the localized impact of chronic CD on the cardiac metabolome of mice infected with two divergent T. cruzi strains. Our data showed chemical differences in localized cardiac regions upon chronic T. cruzi infection, indicating that parasite infection changes the host metabolome at specific sites in chronic CD. These sites were distinct from the sites of highest parasite burden. In addition, we identified acylcarnitines and glycerophosphocholines as discriminatory chemical families within each heart region, comparing infected and uninfected samples. Overall, our study indicated global and positional metabolic differences common to infection with different T. cruzi strains and identified select infection-modulated pathways. These results provide further insight into CD pathogenesis and demonstrate the advantage of a systematic spatial perspective to understand infectious disease tropism. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 10 e0009819 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Danya A Dean Gautham Gautham Jair L Siqueira-Neto James H McKerrow Pieter C Dorrestein Laura-Isobel McCall Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Chagas disease (CD), caused by the parasite Trypanosoma cruzi, is one of nineteen neglected tropical diseases. CD is a vector-borne disease transmitted by triatomines, but CD can also be transmitted through blood transfusions, organ transplants, T. cruzi-contaminated food and drinks, and congenital transmission. While endemic to the Americas, T. cruzi infects 7-8 million people worldwide and can induce severe cardiac symptoms including apical aneurysms, thromboembolisms and arrhythmias during the chronic stage of CD. However, these cardiac clinical manifestations and CD pathogenesis are not fully understood. Using spatial metabolomics (chemical cartography), we sought to understand the localized impact of chronic CD on the cardiac metabolome of mice infected with two divergent T. cruzi strains. Our data showed chemical differences in localized cardiac regions upon chronic T. cruzi infection, indicating that parasite infection changes the host metabolome at specific sites in chronic CD. These sites were distinct from the sites of highest parasite burden. In addition, we identified acylcarnitines and glycerophosphocholines as discriminatory chemical families within each heart region, comparing infected and uninfected samples. Overall, our study indicated global and positional metabolic differences common to infection with different T. cruzi strains and identified select infection-modulated pathways. These results provide further insight into CD pathogenesis and demonstrate the advantage of a systematic spatial perspective to understand infectious disease tropism. |
format |
Article in Journal/Newspaper |
author |
Danya A Dean Gautham Gautham Jair L Siqueira-Neto James H McKerrow Pieter C Dorrestein Laura-Isobel McCall |
author_facet |
Danya A Dean Gautham Gautham Jair L Siqueira-Neto James H McKerrow Pieter C Dorrestein Laura-Isobel McCall |
author_sort |
Danya A Dean |
title |
Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease. |
title_short |
Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease. |
title_full |
Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease. |
title_fullStr |
Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease. |
title_full_unstemmed |
Spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac Chagas Disease. |
title_sort |
spatial metabolomics identifies localized chemical changes in heart tissue during chronic cardiac chagas disease. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009819 https://doaj.org/article/320ec1dc96c94dfa9fe6f452cffc88d9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 10, p e0009819 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009819 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009819 https://doaj.org/article/320ec1dc96c94dfa9fe6f452cffc88d9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009819 |
container_title |
PLOS Neglected Tropical Diseases |
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15 |
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10 |
container_start_page |
e0009819 |
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1766339891242729472 |