The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients
The purpose of this work was to test a cytomegalovirus qualitative PCR and a semi-quantitative PCR on the determination of CMV load in leukocytes of bone marrow and kidney transplanted (RT) patients. Thirty three BMT and 35 RT patients participated of the study. The DNA was subjected to a qualitativ...
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Sociedade Brasileira de Medicina Tropical (SBMT)
2004
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ftdoajarticles:oai:doaj.org/article:320122a2ffc743258a8389015cf1d74f 2023-05-15T15:04:29+02:00 The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients Lauro Juliano Marin Aldo Albuquerque Cunha Victor Hugo Aquino Luiz Tadeu Moraes Figueiredo 2004-03-01T00:00:00Z https://doaj.org/article/320122a2ffc743258a8389015cf1d74f EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000200009&tlng=en https://doaj.org/toc/1678-9849 1678-9849 https://doaj.org/article/320122a2ffc743258a8389015cf1d74f Revista da Sociedade Brasileira de Medicina Tropical, Vol 37, Iss 2, Pp 158-164 (2004) CMV infection Qualitative PCR Quantitative PCR Bone marrow transplant Kidney transplant Arctic medicine. Tropical medicine RC955-962 article 2004 ftdoajarticles 2022-12-30T21:38:58Z The purpose of this work was to test a cytomegalovirus qualitative PCR and a semi-quantitative PCR on the determination of CMV load in leukocytes of bone marrow and kidney transplanted (RT) patients. Thirty three BMT and 35 RT patients participated of the study. The DNA was subjected to a qualitative PCR using primers that amplify part of CMV gB gene. CMV load of positive samples was determined by a semi-quantitative PCR using quantified plasmids inserted with part of the gB gene of CMV as controls. The sensitivity of the test was determined to be 867 plasmid copies/µg DNA. CMV loads between 2,118 and 72,443 copies/µg DNA were observed in 12.1% BMT recipients and between 1,246 and 58,613 copies/µg DNA in 22.9% RT recipients. Further studies are necessary to confirm the usefulness of this CMV semi-quantitative PCR in transplanted patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic The Gib ENVELOPE(-57.531,-57.531,51.817,51.817) |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
CMV infection Qualitative PCR Quantitative PCR Bone marrow transplant Kidney transplant Arctic medicine. Tropical medicine RC955-962 |
spellingShingle |
CMV infection Qualitative PCR Quantitative PCR Bone marrow transplant Kidney transplant Arctic medicine. Tropical medicine RC955-962 Lauro Juliano Marin Aldo Albuquerque Cunha Victor Hugo Aquino Luiz Tadeu Moraes Figueiredo The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients |
topic_facet |
CMV infection Qualitative PCR Quantitative PCR Bone marrow transplant Kidney transplant Arctic medicine. Tropical medicine RC955-962 |
description |
The purpose of this work was to test a cytomegalovirus qualitative PCR and a semi-quantitative PCR on the determination of CMV load in leukocytes of bone marrow and kidney transplanted (RT) patients. Thirty three BMT and 35 RT patients participated of the study. The DNA was subjected to a qualitative PCR using primers that amplify part of CMV gB gene. CMV load of positive samples was determined by a semi-quantitative PCR using quantified plasmids inserted with part of the gB gene of CMV as controls. The sensitivity of the test was determined to be 867 plasmid copies/µg DNA. CMV loads between 2,118 and 72,443 copies/µg DNA were observed in 12.1% BMT recipients and between 1,246 and 58,613 copies/µg DNA in 22.9% RT recipients. Further studies are necessary to confirm the usefulness of this CMV semi-quantitative PCR in transplanted patients. |
format |
Article in Journal/Newspaper |
author |
Lauro Juliano Marin Aldo Albuquerque Cunha Victor Hugo Aquino Luiz Tadeu Moraes Figueiredo |
author_facet |
Lauro Juliano Marin Aldo Albuquerque Cunha Victor Hugo Aquino Luiz Tadeu Moraes Figueiredo |
author_sort |
Lauro Juliano Marin |
title |
The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients |
title_short |
The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients |
title_full |
The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients |
title_fullStr |
The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients |
title_full_unstemmed |
The use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients |
title_sort |
use of qualitative and quantitative polymerase chain reactions for diagnosis of cytomegalovirus infections in bone marrow and kidney transplant recipients |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2004 |
url |
https://doaj.org/article/320122a2ffc743258a8389015cf1d74f |
long_lat |
ENVELOPE(-57.531,-57.531,51.817,51.817) |
geographic |
Arctic The Gib |
geographic_facet |
Arctic The Gib |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 37, Iss 2, Pp 158-164 (2004) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822004000200009&tlng=en https://doaj.org/toc/1678-9849 1678-9849 https://doaj.org/article/320122a2ffc743258a8389015cf1d74f |
_version_ |
1766336242307301376 |