Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom
Abstract Background: Microbial/bacterial resistance against antibiotics poses a serious threat to public health. Furthermore, the side effects of these antibiotics have stimulated tremendous interest in developing new molecules from diverse organisms as therapeutic agents. This study evaluates the a...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:31da554a249d4c09b1abdebd74b2cb9e 2023-05-15T15:14:35+02:00 Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom Shivalingaiah Sudharshan Bhadrapura Lakkappa Dhananjaya 2015-08-01T00:00:00Z https://doi.org/10.1186/s40409-015-0014-y https://doaj.org/article/31da554a249d4c09b1abdebd74b2cb9e EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100322&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0014-y https://doaj.org/article/31da554a249d4c09b1abdebd74b2cb9e Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) Snake venom Bactericidal Antibiotics Drug Human pathogenic bacteria Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2015 ftdoajarticles https://doi.org/10.1186/s40409-015-0014-y 2022-12-31T16:23:43Z Abstract Background: Microbial/bacterial resistance against antibiotics poses a serious threat to public health. Furthermore, the side effects of these antibiotics have stimulated tremendous interest in developing new molecules from diverse organisms as therapeutic agents. This study evaluates the antibacterial potential of a basic protein, Vipera russellii venom phospholipase A 2 fraction VIIIa (VRV-PL-VIIIa), from Daboia russelii pulchella venom against gram-positive and gram-negative bacteria. Methods: The antibacterial potential of VRV-PL-VIIIa in the presence and absence of an inhibitor (p-bromophenacyl bromide) was tested against gram-positive and gram-negative bacteria and the minimum inhibitory concentration was determined by microdilution tests. Results: VRV-PL-VIIIa demonstrated potent antibacterial activities against all the human pathogenic strains tested. It more effectively inhibited such gram-positive bacteria as Staphylococcus aureus and Bacillus subtilis , when compared to the gram-negative bacteria Escherichia coli, Vibrio cholerae, Klebsiella pneumoniae and Salmonella paratyphi . It inhibited bacterial growth at minimum inhibitory concentration values ranging from 11.1 to 19.2 μg/mL. The anti-bacterial potential of VRV-PL-VIIIa was comparable to the standards gentamycin, chlorophenicol and streptomycin. The PLA 2 's hemolytic and antibacterial activities were strongly correlated. Furthermore, even in the presence of p-bromophenacyl bromide, intense antibacterial activity was observed, suggesting a dissociation or partial overlapping of the bactericidal/antimicrobial domains. Conclusion: VRV-PL-VIIIa demonstrated potent antibacterial activities against all the human pathogenic strains tested. The study shows that despite a strong correlation between enzymatic and antimicrobial activities of VRV-PL-VIIIa, it may possess additional properties that mimic the bactericidal/membrane permeability-increasing protein. This study encourages further in-depth studies on the molecular mechanisms of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 21 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Snake venom Bactericidal Antibiotics Drug Human pathogenic bacteria Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Snake venom Bactericidal Antibiotics Drug Human pathogenic bacteria Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Shivalingaiah Sudharshan Bhadrapura Lakkappa Dhananjaya Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom |
topic_facet |
Snake venom Bactericidal Antibiotics Drug Human pathogenic bacteria Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Microbial/bacterial resistance against antibiotics poses a serious threat to public health. Furthermore, the side effects of these antibiotics have stimulated tremendous interest in developing new molecules from diverse organisms as therapeutic agents. This study evaluates the antibacterial potential of a basic protein, Vipera russellii venom phospholipase A 2 fraction VIIIa (VRV-PL-VIIIa), from Daboia russelii pulchella venom against gram-positive and gram-negative bacteria. Methods: The antibacterial potential of VRV-PL-VIIIa in the presence and absence of an inhibitor (p-bromophenacyl bromide) was tested against gram-positive and gram-negative bacteria and the minimum inhibitory concentration was determined by microdilution tests. Results: VRV-PL-VIIIa demonstrated potent antibacterial activities against all the human pathogenic strains tested. It more effectively inhibited such gram-positive bacteria as Staphylococcus aureus and Bacillus subtilis , when compared to the gram-negative bacteria Escherichia coli, Vibrio cholerae, Klebsiella pneumoniae and Salmonella paratyphi . It inhibited bacterial growth at minimum inhibitory concentration values ranging from 11.1 to 19.2 μg/mL. The anti-bacterial potential of VRV-PL-VIIIa was comparable to the standards gentamycin, chlorophenicol and streptomycin. The PLA 2 's hemolytic and antibacterial activities were strongly correlated. Furthermore, even in the presence of p-bromophenacyl bromide, intense antibacterial activity was observed, suggesting a dissociation or partial overlapping of the bactericidal/antimicrobial domains. Conclusion: VRV-PL-VIIIa demonstrated potent antibacterial activities against all the human pathogenic strains tested. The study shows that despite a strong correlation between enzymatic and antimicrobial activities of VRV-PL-VIIIa, it may possess additional properties that mimic the bactericidal/membrane permeability-increasing protein. This study encourages further in-depth studies on the molecular mechanisms of ... |
format |
Article in Journal/Newspaper |
author |
Shivalingaiah Sudharshan Bhadrapura Lakkappa Dhananjaya |
author_facet |
Shivalingaiah Sudharshan Bhadrapura Lakkappa Dhananjaya |
author_sort |
Shivalingaiah Sudharshan |
title |
Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom |
title_short |
Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom |
title_full |
Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom |
title_fullStr |
Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom |
title_full_unstemmed |
Antibacterial potential of a basic phospholipase A 2 (VRV-PL-VIIIa) from Daboia russelii pulchella (Russell's viper) venom |
title_sort |
antibacterial potential of a basic phospholipase a 2 (vrv-pl-viiia) from daboia russelii pulchella (russell's viper) venom |
publisher |
SciELO |
publishDate |
2015 |
url |
https://doi.org/10.1186/s40409-015-0014-y https://doaj.org/article/31da554a249d4c09b1abdebd74b2cb9e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 21, Iss 0 (2015) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992015000100322&lng=en&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1186/s40409-015-0014-y https://doaj.org/article/31da554a249d4c09b1abdebd74b2cb9e |
op_doi |
https://doi.org/10.1186/s40409-015-0014-y |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
21 |
container_issue |
1 |
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1766345018618937344 |