Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil
Introduction Despite the known importance of Clostridium difficile as a nosocomial pathogen, few studies regarding Clostridium difficile infection (CDI) in Brazil have been conducted. To date, the diagnostic tests that are available on the Brazilian market for the diagnosis of CDI have not been eval...
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ftdoajarticles:oai:doaj.org/article:31d1e574434240e39d481990e27d14ed 2023-05-15T15:07:04+02:00 Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil Rodrigo Otávio Silveira Silva Eduardo Garcia Vilela Monique Silva Neves Francisco Carlos Faria Lobato 2014-07-01T00:00:00Z https://doi.org/10.1590/0037-8682-0100-2014 https://doaj.org/article/31d1e574434240e39d481990e27d14ed EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000400447&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0100-2014 https://doaj.org/article/31d1e574434240e39d481990e27d14ed Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 4, Pp 447-450 (2014) Nosocomial Pseudomembranous colitis Diagnosis ELISA Arctic medicine. Tropical medicine RC955-962 article 2014 ftdoajarticles https://doi.org/10.1590/0037-8682-0100-2014 2022-12-31T03:01:13Z Introduction Despite the known importance of Clostridium difficile as a nosocomial pathogen, few studies regarding Clostridium difficile infection (CDI) in Brazil have been conducted. To date, the diagnostic tests that are available on the Brazilian market for the diagnosis of CDI have not been evaluated. The aim of this study was to compare the performances of four commercial methods for the diagnosis of CDI in patients from a university hospital in Brazil. Methods Three enzyme immunoassays (EIAs) and one nucleic acid amplification test (NAAT) were evaluated against a cytotoxicity assay (CTA) and toxigenic culture (TC). Stool samples from 92 patients with suspected CDI were used in this study. Results Twenty-five (27.2%) of 92 samples were positive according to the CTA, and 23 (25%) were positive according to the TC. All EIAs and the NAAT test demonstrated sensitivities between 59 and 68% and specificities greater than 91%. Conclusions All four methods exhibited low sensitivities for the diagnosis of CDI, which could lead to a large number of false-negative results, an increased risk of cross-infection to other patients, and overtreatment with empirical antibiotics. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 47 4 447 450 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Nosocomial Pseudomembranous colitis Diagnosis ELISA Arctic medicine. Tropical medicine RC955-962 |
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Nosocomial Pseudomembranous colitis Diagnosis ELISA Arctic medicine. Tropical medicine RC955-962 Rodrigo Otávio Silveira Silva Eduardo Garcia Vilela Monique Silva Neves Francisco Carlos Faria Lobato Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil |
topic_facet |
Nosocomial Pseudomembranous colitis Diagnosis ELISA Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction Despite the known importance of Clostridium difficile as a nosocomial pathogen, few studies regarding Clostridium difficile infection (CDI) in Brazil have been conducted. To date, the diagnostic tests that are available on the Brazilian market for the diagnosis of CDI have not been evaluated. The aim of this study was to compare the performances of four commercial methods for the diagnosis of CDI in patients from a university hospital in Brazil. Methods Three enzyme immunoassays (EIAs) and one nucleic acid amplification test (NAAT) were evaluated against a cytotoxicity assay (CTA) and toxigenic culture (TC). Stool samples from 92 patients with suspected CDI were used in this study. Results Twenty-five (27.2%) of 92 samples were positive according to the CTA, and 23 (25%) were positive according to the TC. All EIAs and the NAAT test demonstrated sensitivities between 59 and 68% and specificities greater than 91%. Conclusions All four methods exhibited low sensitivities for the diagnosis of CDI, which could lead to a large number of false-negative results, an increased risk of cross-infection to other patients, and overtreatment with empirical antibiotics. |
format |
Article in Journal/Newspaper |
author |
Rodrigo Otávio Silveira Silva Eduardo Garcia Vilela Monique Silva Neves Francisco Carlos Faria Lobato |
author_facet |
Rodrigo Otávio Silveira Silva Eduardo Garcia Vilela Monique Silva Neves Francisco Carlos Faria Lobato |
author_sort |
Rodrigo Otávio Silveira Silva |
title |
Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil |
title_short |
Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil |
title_full |
Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil |
title_fullStr |
Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil |
title_full_unstemmed |
Evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of Clostridium difficile-associated diarrhea at a university hospital in Brazil |
title_sort |
evaluation of three enzyme immunoassays and a nucleic acid amplification test for the diagnosis of clostridium difficile-associated diarrhea at a university hospital in brazil |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2014 |
url |
https://doi.org/10.1590/0037-8682-0100-2014 https://doaj.org/article/31d1e574434240e39d481990e27d14ed |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 47, Iss 4, Pp 447-450 (2014) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822014000400447&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0100-2014 https://doaj.org/article/31d1e574434240e39d481990e27d14ed |
op_doi |
https://doi.org/10.1590/0037-8682-0100-2014 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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47 |
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447 |
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