Norovirus Persistence in Oysters to Prolonged Commercial Purification
Depuration is generally the main treatment employed for bivalve mollusks harvested from contaminated sites. Commercial depuration has demonstrated to be effective for removal of bacterial pathogens, although it probably provides only limited efficacy against human enteric viruses. We evaluated the q...
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MDPI AG
2021
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ftdoajarticles:oai:doaj.org/article:31ccd794cab146af9ae4c20fc21ee4e1 2023-05-15T15:58:43+02:00 Norovirus Persistence in Oysters to Prolonged Commercial Purification Roberta Battistini Chiara Masotti Valeria Listorti Elisabetta Suffredini Cristiana Maurella Aitor Garcia-Vozmediano Erica Costa Francesco Iacona Mino Orlandi Carlo Ercolini Laura Serracca 2021-07-01T00:00:00Z https://doi.org/10.3390/pathogens10080944 https://doaj.org/article/31ccd794cab146af9ae4c20fc21ee4e1 EN eng MDPI AG https://www.mdpi.com/2076-0817/10/8/944 https://doaj.org/toc/2076-0817 doi:10.3390/pathogens10080944 2076-0817 https://doaj.org/article/31ccd794cab146af9ae4c20fc21ee4e1 Pathogens, Vol 10, Iss 944, p 944 (2021) Crassostrea gigas depuration norovirus food safety real-time qPCR Medicine R article 2021 ftdoajarticles https://doi.org/10.3390/pathogens10080944 2022-12-30T20:28:11Z Depuration is generally the main treatment employed for bivalve mollusks harvested from contaminated sites. Commercial depuration has demonstrated to be effective for removal of bacterial pathogens, although it probably provides only limited efficacy against human enteric viruses. We evaluated the quantitative reduction of norovirus (NoV) genogroups I and II in naturally contaminated oysters after 1, 4, and 9 days of depuration. The process was conducted in an authorized depuration plant, and NoV concentration was determined by RT-qPCR according to ISO 15216-1:2017 method. Regardless of the NoV genogroup, our results showed no significant reduction in NoV concentration after 1 day of depuration. Higher mean reduction (68%) was obtained after 4 days of treatment, while no further increase was observed after 9 days. Overall, reduction was highly variable, and none of the trials showed statistically significant reduction in NoV RNA concentration at the end of each depuration period. Indeed, NoV concentration remained high in 70% of samples even after 9 days of depuration, with values ranging between 4.0 × 10 2 and 2.3 × 10 4 g.c./g. These results indicate that an extension of commercial depuration time does not appear to be effective for reducing or eliminating NoV in oysters. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Pathogens 10 8 944 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Crassostrea gigas depuration norovirus food safety real-time qPCR Medicine R |
spellingShingle |
Crassostrea gigas depuration norovirus food safety real-time qPCR Medicine R Roberta Battistini Chiara Masotti Valeria Listorti Elisabetta Suffredini Cristiana Maurella Aitor Garcia-Vozmediano Erica Costa Francesco Iacona Mino Orlandi Carlo Ercolini Laura Serracca Norovirus Persistence in Oysters to Prolonged Commercial Purification |
topic_facet |
Crassostrea gigas depuration norovirus food safety real-time qPCR Medicine R |
description |
Depuration is generally the main treatment employed for bivalve mollusks harvested from contaminated sites. Commercial depuration has demonstrated to be effective for removal of bacterial pathogens, although it probably provides only limited efficacy against human enteric viruses. We evaluated the quantitative reduction of norovirus (NoV) genogroups I and II in naturally contaminated oysters after 1, 4, and 9 days of depuration. The process was conducted in an authorized depuration plant, and NoV concentration was determined by RT-qPCR according to ISO 15216-1:2017 method. Regardless of the NoV genogroup, our results showed no significant reduction in NoV concentration after 1 day of depuration. Higher mean reduction (68%) was obtained after 4 days of treatment, while no further increase was observed after 9 days. Overall, reduction was highly variable, and none of the trials showed statistically significant reduction in NoV RNA concentration at the end of each depuration period. Indeed, NoV concentration remained high in 70% of samples even after 9 days of depuration, with values ranging between 4.0 × 10 2 and 2.3 × 10 4 g.c./g. These results indicate that an extension of commercial depuration time does not appear to be effective for reducing or eliminating NoV in oysters. |
format |
Article in Journal/Newspaper |
author |
Roberta Battistini Chiara Masotti Valeria Listorti Elisabetta Suffredini Cristiana Maurella Aitor Garcia-Vozmediano Erica Costa Francesco Iacona Mino Orlandi Carlo Ercolini Laura Serracca |
author_facet |
Roberta Battistini Chiara Masotti Valeria Listorti Elisabetta Suffredini Cristiana Maurella Aitor Garcia-Vozmediano Erica Costa Francesco Iacona Mino Orlandi Carlo Ercolini Laura Serracca |
author_sort |
Roberta Battistini |
title |
Norovirus Persistence in Oysters to Prolonged Commercial Purification |
title_short |
Norovirus Persistence in Oysters to Prolonged Commercial Purification |
title_full |
Norovirus Persistence in Oysters to Prolonged Commercial Purification |
title_fullStr |
Norovirus Persistence in Oysters to Prolonged Commercial Purification |
title_full_unstemmed |
Norovirus Persistence in Oysters to Prolonged Commercial Purification |
title_sort |
norovirus persistence in oysters to prolonged commercial purification |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/pathogens10080944 https://doaj.org/article/31ccd794cab146af9ae4c20fc21ee4e1 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Pathogens, Vol 10, Iss 944, p 944 (2021) |
op_relation |
https://www.mdpi.com/2076-0817/10/8/944 https://doaj.org/toc/2076-0817 doi:10.3390/pathogens10080944 2076-0817 https://doaj.org/article/31ccd794cab146af9ae4c20fc21ee4e1 |
op_doi |
https://doi.org/10.3390/pathogens10080944 |
container_title |
Pathogens |
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10 |
container_issue |
8 |
container_start_page |
944 |
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1766394492931276800 |