Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar
INTRODUCTION: Opportunistic fungal infections in immunocompromised hosts are caused by Candida species, and the majority of such infections are due to Candida albicans. However, the emerging pathogen Candida dubliniensis demonstrates several phenotypic characteristics in common with C. albicans, suc...
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ftdoajarticles:oai:doaj.org/article:31cbedb6534a4119a9254586971cf217 2023-05-15T15:08:07+02:00 Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar Fabíola Silveira-Gomes Dayse Nogueira Sarmento Elaine Patrícia Tavares do Espírito-Santo Nádia de Oliveira Souza Thifany Mendes Pinto Silvia Helena Marques-da-Silva 2011-08-01T00:00:00Z https://doi.org/10.1590/S0037-86822011000400011 https://doaj.org/article/31cbedb6534a4119a9254586971cf217 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000400011&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822011000400011 https://doaj.org/article/31cbedb6534a4119a9254586971cf217 Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 4, Pp 457-460 (2011) Identificação bioquímica Características fenotípicas Candida albicans Arctic medicine. Tropical medicine RC955-962 article 2011 ftdoajarticles https://doi.org/10.1590/S0037-86822011000400011 2022-12-30T21:26:05Z INTRODUCTION: Opportunistic fungal infections in immunocompromised hosts are caused by Candida species, and the majority of such infections are due to Candida albicans. However, the emerging pathogen Candida dubliniensis demonstrates several phenotypic characteristics in common with C. albicans, such as production of germ tubes and chlamydospores, calling attention to the development of stable resistance to fluconazole in vitro. The aim of this study was to evaluate the performance of biochemistry identification in the differentiating between C. albicans and C. dubliniensis, by phenotyping of yeast identified as C. albicans. METHODS: Seventy-nine isolates identified as C. albicans by the API system ID 32C were grown on Sabouraud dextrose agar at 30°C for 24-48h and then inoculated on hypertonic Sabouraud broth and tobacco agar. RESULTS: Our results showed that 17 (21.5%) isolates were growth-inhibited on hypertonic Sabouraud broth, a phenotypic trait inconsistent with C. albicans in this medium. However, the results observed on tobacco agar showed that only 9 (11.4%) of the growth-inhibited isolates produced characteristic colonies of C. dubliniensis (rough colonies, yellowish-brown with abundant fragments of hyphae and chlamydospores). CONCLUSIONS: The results suggest that this method is a simple tool for screening C. albicans and non-albicans yeast and for verification of automated identification. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 44 4 457 460 |
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Identificação bioquímica Características fenotípicas Candida albicans Arctic medicine. Tropical medicine RC955-962 |
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Identificação bioquímica Características fenotípicas Candida albicans Arctic medicine. Tropical medicine RC955-962 Fabíola Silveira-Gomes Dayse Nogueira Sarmento Elaine Patrícia Tavares do Espírito-Santo Nádia de Oliveira Souza Thifany Mendes Pinto Silvia Helena Marques-da-Silva Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar |
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Identificação bioquímica Características fenotípicas Candida albicans Arctic medicine. Tropical medicine RC955-962 |
description |
INTRODUCTION: Opportunistic fungal infections in immunocompromised hosts are caused by Candida species, and the majority of such infections are due to Candida albicans. However, the emerging pathogen Candida dubliniensis demonstrates several phenotypic characteristics in common with C. albicans, such as production of germ tubes and chlamydospores, calling attention to the development of stable resistance to fluconazole in vitro. The aim of this study was to evaluate the performance of biochemistry identification in the differentiating between C. albicans and C. dubliniensis, by phenotyping of yeast identified as C. albicans. METHODS: Seventy-nine isolates identified as C. albicans by the API system ID 32C were grown on Sabouraud dextrose agar at 30°C for 24-48h and then inoculated on hypertonic Sabouraud broth and tobacco agar. RESULTS: Our results showed that 17 (21.5%) isolates were growth-inhibited on hypertonic Sabouraud broth, a phenotypic trait inconsistent with C. albicans in this medium. However, the results observed on tobacco agar showed that only 9 (11.4%) of the growth-inhibited isolates produced characteristic colonies of C. dubliniensis (rough colonies, yellowish-brown with abundant fragments of hyphae and chlamydospores). CONCLUSIONS: The results suggest that this method is a simple tool for screening C. albicans and non-albicans yeast and for verification of automated identification. |
format |
Article in Journal/Newspaper |
author |
Fabíola Silveira-Gomes Dayse Nogueira Sarmento Elaine Patrícia Tavares do Espírito-Santo Nádia de Oliveira Souza Thifany Mendes Pinto Silvia Helena Marques-da-Silva |
author_facet |
Fabíola Silveira-Gomes Dayse Nogueira Sarmento Elaine Patrícia Tavares do Espírito-Santo Nádia de Oliveira Souza Thifany Mendes Pinto Silvia Helena Marques-da-Silva |
author_sort |
Fabíola Silveira-Gomes |
title |
Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar |
title_short |
Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar |
title_full |
Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar |
title_fullStr |
Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar |
title_full_unstemmed |
Differentiation between Candida albicans and Candida dubliniensis using hypertonic Sabouraud broth and tobacco agar |
title_sort |
differentiation between candida albicans and candida dubliniensis using hypertonic sabouraud broth and tobacco agar |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
publishDate |
2011 |
url |
https://doi.org/10.1590/S0037-86822011000400011 https://doaj.org/article/31cbedb6534a4119a9254586971cf217 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 44, Iss 4, Pp 457-460 (2011) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822011000400011&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/S0037-86822011000400011 https://doaj.org/article/31cbedb6534a4119a9254586971cf217 |
op_doi |
https://doi.org/10.1590/S0037-86822011000400011 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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44 |
container_issue |
4 |
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457 |
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460 |
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