Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china
Abstract In order to effectively identify the vaccine and field strains of Canine distemper virus (CDV), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). We selected an 829 bp...
Published in: | Virology Journal |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2011
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1743-422X-8-85 https://doaj.org/article/3183f9e8721b42a68e91350c95090114 |
id |
ftdoajarticles:oai:doaj.org/article:3183f9e8721b42a68e91350c95090114 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:3183f9e8721b42a68e91350c95090114 2023-05-15T15:07:11+02:00 Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china Zhao Jianjun Zhang Hailing Chai Xiuli Yan Xijun Wang Fengxue Wen Yongjun Wu Wei 2011-02-01T00:00:00Z https://doi.org/10.1186/1743-422X-8-85 https://doaj.org/article/3183f9e8721b42a68e91350c95090114 EN eng BMC http://www.virologyj.com/content/8/1/85 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-8-85 1743-422X https://doaj.org/article/3183f9e8721b42a68e91350c95090114 Virology Journal, Vol 8, Iss 1, p 85 (2011) Infectious and parasitic diseases RC109-216 article 2011 ftdoajarticles https://doi.org/10.1186/1743-422X-8-85 2022-12-31T07:12:54Z Abstract In order to effectively identify the vaccine and field strains of Canine distemper virus (CDV), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). We selected an 829 bp fragment of the nucleoprotein (N) gene of CDV. By RFLP analysis using Bam HI, field isolates were distinguishable from the vaccine strains. Two fragments were obtained from the vaccine strains by RT-PCR-RFLP analysis while three were observed in the field strains. An 829 nucleotide region of the CDV N gene was analyzed in 19 CDV field strains isolated from minks, raccoon dogs and foxes in China between 2005 and 2007. The results suggest this method is precise, accurate and efficient. It was also determined that three different genotypes exist in CDV field strains in fur animal herds of the north of China, most of which belong to Asian type. Mutated field strains, JSY06-R1, JSY06-R2 and JDH07-F1 also exist in Northern China, but are most closely related to the standard virulent strain A75/17, designated in Arctic and America-2 genetype in the present study, respectively. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Virology Journal 8 1 85 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Infectious and parasitic diseases RC109-216 |
spellingShingle |
Infectious and parasitic diseases RC109-216 Zhao Jianjun Zhang Hailing Chai Xiuli Yan Xijun Wang Fengxue Wen Yongjun Wu Wei Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
topic_facet |
Infectious and parasitic diseases RC109-216 |
description |
Abstract In order to effectively identify the vaccine and field strains of Canine distemper virus (CDV), a new differential diagnostic test has been developed based on reverse transcription-polymerase chain reaction (RT-PCR) and restriction fragment length polymorphism (RFLP). We selected an 829 bp fragment of the nucleoprotein (N) gene of CDV. By RFLP analysis using Bam HI, field isolates were distinguishable from the vaccine strains. Two fragments were obtained from the vaccine strains by RT-PCR-RFLP analysis while three were observed in the field strains. An 829 nucleotide region of the CDV N gene was analyzed in 19 CDV field strains isolated from minks, raccoon dogs and foxes in China between 2005 and 2007. The results suggest this method is precise, accurate and efficient. It was also determined that three different genotypes exist in CDV field strains in fur animal herds of the north of China, most of which belong to Asian type. Mutated field strains, JSY06-R1, JSY06-R2 and JDH07-F1 also exist in Northern China, but are most closely related to the standard virulent strain A75/17, designated in Arctic and America-2 genetype in the present study, respectively. |
format |
Article in Journal/Newspaper |
author |
Zhao Jianjun Zhang Hailing Chai Xiuli Yan Xijun Wang Fengxue Wen Yongjun Wu Wei |
author_facet |
Zhao Jianjun Zhang Hailing Chai Xiuli Yan Xijun Wang Fengxue Wen Yongjun Wu Wei |
author_sort |
Zhao Jianjun |
title |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_short |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_full |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_fullStr |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_full_unstemmed |
Differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
title_sort |
differentiation of canine distemper virus isolates in fur animals from various vaccine strains by reverse transcription-polymerase chain reaction-restriction fragment length polymorphism according to phylogenetic relations in china |
publisher |
BMC |
publishDate |
2011 |
url |
https://doi.org/10.1186/1743-422X-8-85 https://doaj.org/article/3183f9e8721b42a68e91350c95090114 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Virology Journal, Vol 8, Iss 1, p 85 (2011) |
op_relation |
http://www.virologyj.com/content/8/1/85 https://doaj.org/toc/1743-422X doi:10.1186/1743-422X-8-85 1743-422X https://doaj.org/article/3183f9e8721b42a68e91350c95090114 |
op_doi |
https://doi.org/10.1186/1743-422X-8-85 |
container_title |
Virology Journal |
container_volume |
8 |
container_issue |
1 |
container_start_page |
85 |
_version_ |
1766338736787816448 |