MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis
Abstract Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotyp...
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ftdoajarticles:oai:doaj.org/article:313aa23f44594fde8f10925b0e4fbe16 2023-05-15T15:59:54+02:00 MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis Jacques Isabelle Al Dahouk Sascha Djønne Berit Ramisse Françoise Tryland Morten Foster Geoffrey Le Flèche Philippe Maquart Marianne Neubauer Heinrich Walravens Karl Godfroid Jacques Cloeckaert Axel Vergnaud Gilles 2009-07-01T00:00:00Z https://doi.org/10.1186/1471-2180-9-145 https://doaj.org/article/313aa23f44594fde8f10925b0e4fbe16 EN eng BMC http://www.biomedcentral.com/1471-2180/9/145 https://doaj.org/toc/1471-2180 doi:10.1186/1471-2180-9-145 1471-2180 https://doaj.org/article/313aa23f44594fde8f10925b0e4fbe16 BMC Microbiology, Vol 9, Iss 1, p 145 (2009) Microbiology QR1-502 article 2009 ftdoajarticles https://doi.org/10.1186/1471-2180-9-145 2022-12-31T06:55:22Z Abstract Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. Results 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals ( Cystophora cristata ) and the two others comprising other seal species isolates. Conclusion The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest ... Article in Journal/Newspaper Cystophora cristata minke whale Directory of Open Access Journals: DOAJ Articles New Zealand BMC Microbiology 9 1 145 |
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Microbiology QR1-502 |
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Microbiology QR1-502 Jacques Isabelle Al Dahouk Sascha Djønne Berit Ramisse Françoise Tryland Morten Foster Geoffrey Le Flèche Philippe Maquart Marianne Neubauer Heinrich Walravens Karl Godfroid Jacques Cloeckaert Axel Vergnaud Gilles MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
topic_facet |
Microbiology QR1-502 |
description |
Abstract Background Since 1994, Brucella strains have been isolated from a wide range of marine mammals. They are currently recognized as two new Brucella species, B. pinnipedialis for the pinniped isolates and B. ceti for the cetacean isolates in agreement with host preference and specific phenotypic and molecular markers. In order to investigate the genetic relationships within the marine mammal Brucella isolates and with reference to terrestrial mammal Brucella isolates, we applied in this study the Multiple Loci VNTR (Variable Number of Tandem Repeats) Analysis (MLVA) approach. A previously published assay comprising 16 loci (MLVA-16) that has been shown to be highly relevant and efficient for typing and clustering Brucella strains from animal and human origin was used. Results 294 marine mammal Brucella strains collected in European waters from 173 animals and a human isolate from New Zealand presumably from marine origin were investigated by MLVA-16. Marine mammal Brucella isolates were shown to be different from the recognized terrestrial mammal Brucella species and biovars and corresponded to 3 major related groups, one specific of the B. ceti strains, one of the B. pinnipedialis strains and the last composed of the human isolate. In the B. ceti group, 3 subclusters were identified, distinguishing a cluster of dolphin, minke whale and porpoise isolates and two clusters mostly composed of dolphin isolates. These results were in accordance with published analyses using other phenotypic or molecular approaches, or different panels of VNTR loci. The B. pinnipedialis group could be similarly subdivided in 3 subclusters, one composed exclusively of isolates from hooded seals ( Cystophora cristata ) and the two others comprising other seal species isolates. Conclusion The clustering analysis of a large collection of marine mammal Brucella isolates from European waters significantly strengthens the current view of the population structure of these two species, and their relative position with respect to the rest ... |
format |
Article in Journal/Newspaper |
author |
Jacques Isabelle Al Dahouk Sascha Djønne Berit Ramisse Françoise Tryland Morten Foster Geoffrey Le Flèche Philippe Maquart Marianne Neubauer Heinrich Walravens Karl Godfroid Jacques Cloeckaert Axel Vergnaud Gilles |
author_facet |
Jacques Isabelle Al Dahouk Sascha Djønne Berit Ramisse Françoise Tryland Morten Foster Geoffrey Le Flèche Philippe Maquart Marianne Neubauer Heinrich Walravens Karl Godfroid Jacques Cloeckaert Axel Vergnaud Gilles |
author_sort |
Jacques Isabelle |
title |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_short |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_full |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_fullStr |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_full_unstemmed |
MLVA-16 typing of 295 marine mammal Brucella isolates from different animal and geographic origins identifies 7 major groups within Brucella ceti and Brucella pinnipedialis |
title_sort |
mlva-16 typing of 295 marine mammal brucella isolates from different animal and geographic origins identifies 7 major groups within brucella ceti and brucella pinnipedialis |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1471-2180-9-145 https://doaj.org/article/313aa23f44594fde8f10925b0e4fbe16 |
geographic |
New Zealand |
geographic_facet |
New Zealand |
genre |
Cystophora cristata minke whale |
genre_facet |
Cystophora cristata minke whale |
op_source |
BMC Microbiology, Vol 9, Iss 1, p 145 (2009) |
op_relation |
http://www.biomedcentral.com/1471-2180/9/145 https://doaj.org/toc/1471-2180 doi:10.1186/1471-2180-9-145 1471-2180 https://doaj.org/article/313aa23f44594fde8f10925b0e4fbe16 |
op_doi |
https://doi.org/10.1186/1471-2180-9-145 |
container_title |
BMC Microbiology |
container_volume |
9 |
container_issue |
1 |
container_start_page |
145 |
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1766395793141399552 |